Endothelial cell adhesion and blood response to hemocompatible peptide 1 (HCP-1), REDV, and RGD peptide sequences with free N-terminal amino groups immobilized on a biomedical expanded polytetrafluorethylene surface

2021 ◽  
Author(s):  
Yihua Liu ◽  
Atsushi Mahara ◽  
Yusuke Kambe ◽  
Yu-I. Hsu ◽  
Tetsuji Yamaoka
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Rgd Peptide ◽  
Terminal Cell ◽  
Amino Groups ◽  
Cell Adhesive ◽  
Terminal Amino ◽  
Endothelial Cell Adhesion

Free N-terminal cell adhesive peptides are assessed from the viewpoint of endothelial cell adhesion and blood response.

Synthesis, Surface and Cell-Adhesion Properties of Polyurethanes Containing Covalently Grafted RGD-Peptides

1993 ◽  
Vol 331 ◽  
Author(s):  
Horng-Ban Lin ◽  
Stuart L. Cooper
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Cell Growth ◽  
Culture Medium ◽  
Cell Attachment ◽  
Grafted Polymers ◽  
Cell Adhesive ◽  
Endothelial Cell Growth ◽  
Endothelial Cell Adhesion

AbstractIn an attempt to improve endothelial cell adhesion and growth on a polyurethane copolymer, cell adhesive RGD-containing peptides were grafted to the polymer backbone. Two peptide grafting reaction schemes, including one-step and two-step approaches, were developed. Amino acid analysis confirmed that the two-step approach had a higher peptide coupling efficiency. The two-step reaction scheme was utilized to prepare GRGDSY, GRGDVY and GRGESY (inactive control) peptide grafted polyurethanes with two different peptide densities (100 and 250 μmol/g polymer). Dynamic contact angle measurements indicated that the surfaces of the peptide grafted polyurethanes were more hydrophilic than the starting and carboxylated versions of the precursor polyurethane. In-vitro endothelial cell adhesion experiments showed that, without the presence of serum in culture medium, the GRGDSY- and GRGDVY-grafted polyurethanes dramatically enhanced cell attachment and spreading. Increasing the peptide density from 100 to 250 μmol/g polymer for the GRGDSYand GRGDVY-grafted polyurethanes resulted in an increase in cell attachment. With approximately the same peptide density (100 or 250 μmol/g polymer), the GRGDVY-grafted polymers supported more adherent cells than the GRGDSY-grafted polymers. Similar trends were observed in the in-vitro endothelial cell growth studies using culture medium containing serum and endothelial cell growth supplement. These RGD-peptide grafted polyurethanes may be useful in providing an easily prepared cell-adhesive substrate for various implantable devices and hybrid organs.

scholarly journals A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces

Biomaterials ◽  
2014 ◽  
Vol 35 (3) ◽  
pp. 879-890 ◽  
Author(s):  
Corinne A. Hoesli ◽  
Alain Garnier ◽  
Pierre-Marc Juneau ◽  
Pascale Chevallier ◽  
Carl Duchesne ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Rgd Peptide ◽  
Endothelial Cell Adhesion

Differential Expression of Platelet-Endothelial Cell Adhesion Molecule-1 (PECAM-1) in Murine Tissues

1998 ◽  
Vol 5 (2-3) ◽  
pp. 179-188 ◽  
Author(s):  
MICHAEL J EPPIHIMER ◽  
J A N I C E RUSELL ◽  
R O B E R T LANGLEY ◽  
G I N A VALLIEN ◽  
DONALD C ANDERSON ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Differential Expression ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Endothelial Cell Adhesion Molecule ◽  
Platelet Endothelial Cell Adhesion ◽  
Cell Adhesion Molecule 1 ◽  
Endothelial Cell Adhesion
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