scholarly journals Combined effect of shear stress and laser-patterned topography on Schwann cell outgrowth: synergistic or antagonistic?

2021 ◽  
Author(s):  
Eleftheria Babaliari ◽  
Paraskevi Kavatzikidou ◽  
Anna Mitraki ◽  
Yannis Papaharilaou ◽  
Anthi Ranella ◽  
...  
Keyword(s):  
Shear Stress ◽  
Schwann Cell ◽  
Combined Effect ◽  
Specific Cell ◽  
Cell Outgrowth ◽  
Cell Responses ◽  
Topographical Cues

Shear stress can act either synergistically or antagonistically with topographical cues in specific cell responses such as orientation and elongation.

Combined effects of multi-scale topographical cues on stable cell sheet formation and differentiation of mesenchymal stem cells

2017 ◽  
Vol 5 (10) ◽  
pp. 2056-2067 ◽  
Author(s):  
Sisi Li ◽  
Shreyas Kuddannaya ◽  
Yon Jin Chuah ◽  
Jingnan Bao ◽  
Yilei Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Sheet ◽  
Specific Cell ◽  
Combined Effects ◽  
Multi Scale ◽  
Cell Responses ◽  
Topographical Cues

To decipher specific cell responses to diverse and complex in vivo signals, it is essential to emulate specific surface chemicals, extra cellular matrix (ECM) components and topographical signals through reliable and easily reproducible in vitro systems.

scholarly journals Interleukin-4 Regulates the Expression of CD209 and Subsequent Uptake of Mycobacterium leprae by Schwann Cells in Human Leprosy

2010 ◽  
Vol 78 (11) ◽  
pp. 4634-4643 ◽  
Author(s):  
Rosane M. B. Teles ◽  
Stephan R. Krutzik ◽  
Maria T. Ochoa ◽  
Rosane B. Oliveira ◽  
Euzenir N. Sarno ◽  
...  
Keyword(s):  
Schwann Cell ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Primary Cultures ◽  
Mycobacterium Leprae ◽  
Microbial Pathogens ◽  
Interleukin 4 ◽  
Common Mechanism ◽  
Specific Cell

ABSTRACT The ability of microbial pathogens to target specific cell types is a key aspect of the pathogenesis of infectious disease. Mycobacterium leprae, by infecting Schwann cells, contributes to nerve injury in patients with leprosy. Here, we investigated mechanisms of host-pathogen interaction in the peripheral nerve lesions of leprosy. We found that the expression of the C-type lectin, CD209, known to be expressed on tissue macrophages and to mediate the uptake of M. leprae, was present on Schwann cells, colocalizing with the Schwann cell marker, CNPase (2′,3′-cyclic nucleotide 3′-phosphodiesterase), along with the M. leprae antigen PGL-1 in the peripheral nerve biopsy specimens. In vitro, human CD209-positive Schwann cells, both from primary cultures and a long-term line, have a higher binding of M. leprae compared to CD209-negative Schwann cells. Interleukin-4, known to be expressed in skin lesions from multibacillary patients, increased CD209 expression on human Schwann cells and subsequent Schwann cell binding to M. leprae, whereas Th1 cytokines did not induce CD209 expression on these cells. Therefore, the regulated expression of CD209 represents a common mechanism by which Schwann cells and macrophages bind and take up M. leprae, contributing to the pathogenesis of leprosy.

scholarly journals Induction of Genotype Cross-Reactive, Hepatitis C Virus-Specific, Cell-Mediated Immunity in DNA-Vaccinated Mice

2018 ◽  
Vol 92 (8) ◽  
pp. e02133-17 ◽  
Author(s):  
Danushka K. Wijesundara ◽  
Jason Gummow ◽  
Yanrui Li ◽  
Wenbo Yu ◽  
Benjamin J. Quah ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Dna Vaccine ◽  
Target Cells ◽  
Cell Mediated Immunity ◽  
Specific Cell ◽  
Universal Vaccine ◽  
Cell Responses ◽  
Single Genotype

ABSTRACTA universal hepatitis C virus (HCV) vaccine should elicit multiantigenic, multigenotypic responses, which are more likely to protect against challenge with the range of genotypes and subtypes circulating in the community. A vaccine cocktail and vaccines encoding consensus HCV sequences are attractive approaches to achieve this goal. Consequently, in a series of mouse vaccination studies, we compared the immunogenicity of a DNA vaccine encoding a consensus HCV nonstructural 5B (NS5B) protein to that of a cocktail of DNA plasmids encoding the genotype 1b (Gt1b) and Gt3a NS5B proteins. To complement this study, we assessed responses to a multiantigenic cocktail regimen by comparing a DNA vaccine cocktail encoding Gt1b and Gt3a NS3, NS4, and NS5B proteins to a single-genotype NS3/4/5B DNA vaccine. To thoroughly evaluatein vivocytotoxic T lymphocyte (CTL) and T helper (Th) cell responses against Gt1b and Gt3a HCV peptide-pulsed target cells, we exploited a novel fluorescent-target array (FTA). FTA and enzyme-linked immunosorbent spot (ELISpot) analyses collectively indicated that the cocktail regimens elicited higher responses to Gt1b and Gt3a NS5B proteins than those with the consensus vaccine, while the multiantigenic DNA cocktail significantly increased the responses to NS3 and NS5B compared to those elicited by the single-genotype vaccines. Thus, a DNA cocktail vaccination regimen is more effective than a consensus vaccine or a monovalent vaccine at increasing the breadth of multigenotypic T cell responses, which has implications for the development of vaccines for communities where multiple HCV genotypes circulate.IMPORTANCEDespite the development of highly effective direct-acting antivirals (DAA), infections with hepatitis C virus (HCV) continue, particularly in countries where the supply of DAA is limited. Furthermore, patients who eliminate the virus as a result of DAA therapy can still be reinfected. Thus, a vaccine for HCV is urgently required, but the heterogeneity of HCV strains makes the development of a universal vaccine difficult. To address this, we developed a novel cytolytic DNA vaccine which elicits robust cell-mediated immunity (CMI) to the nonstructural (NS) proteins in vaccinated animals. We compared the immune responses against genotypes 1 and 3 that were elicited by a consensus DNA vaccine or a DNA vaccine cocktail and showed that the cocktail induced higher levels of CMI to the NS proteins of both genotypes. This study suggests that a universal HCV vaccine can most readily be achieved by use of a DNA vaccine cocktail.

Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses

Biomaterials ◽  
2014 ◽  
Vol 35 (21) ◽  
pp. 5619-5626 ◽  
Author(s):  
Elisa Boanini ◽  
Paola Torricelli ◽  
Massimo Gazzano ◽  
Elena Della Bella ◽  
Milena Fini ◽  
...  
Keyword(s):  
Bone Cell ◽  
Combined Effect ◽  
Cell Responses

scholarly journals GammalFN Ehrlichia canis-specific cell responses

2009 ◽  
Vol 15 ◽  
pp. 70-71 ◽  
Author(s):  
T. Normand ◽  
L. Forest ◽  
L. Chabanne ◽  
S. Richard ◽  
B. Davoust ◽  
...  
Keyword(s):  
Ehrlichia Canis ◽  
Specific Cell ◽  
Cell Responses

Anisotropic, Degradable Polymer Assemblies Driven by a Rigid Hydrogen-Bonding Motif That Induce Shape-Specific Cell Responses

2021 ◽  
Author(s):  
Kazuki Fukushima ◽  
Kodai Matsuzaki ◽  
Masashi Oji ◽  
Yuji Higuchi ◽  
Go Watanabe ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Specific Cell ◽  
Degradable Polymer ◽  
Cell Responses

Integration of signal-transduction processes

1988 ◽  
Vol 66 (6) ◽  
pp. 557-566 ◽  
Author(s):  
Marilyn J. Mooibroek ◽  
Jerry H. Wang
Keyword(s):  
Signal Transduction ◽  
Information Transfer ◽  
Fine Tuning ◽  
External Signal ◽  
Specific Cell ◽  
Cell Stimulation ◽  
Cell Responses ◽  
Almost All ◽  
Signal Cascades ◽  
Reaction Cascades

The adenylate cyclase – cAMP, phospholipase C – IP3 (inositol 1,4,5-triphosphate), and DAG (diacylglycerol) signal transduction systems are used to illustrate general principles underlying the process of information transfer during cell stimulation. Both systems consist of reaction cascades that convert the external signal to an intracellular messenger, translate the messenger to regulatory activities, and then modulate the activities of appropriate cellular proteins to result in specific cell responses. Almost all of these reactions are under second-messenger-dependent regulation, with many being regulated by multiple messengers. Such complex regulation provides ample opportunities for the fine-tuning of the signal cascades and for coordination between cascades during cell stimulation. Specific examples are used to illustrate how the cell uses different intrasystem and intersystem regulatory reactions to achieve specific responses.

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