An alpha/beta chimeric peptide molecular brush for eradicating MRSA biofilms and persister cells to mitigate antimicrobial resistance

2020 ◽  
Vol 8 (24) ◽  
pp. 6883-6889 ◽  
Author(s):  
Ximian Xiao ◽  
Si Zhang ◽  
Sheng Chen ◽  
Yuxin Qian ◽  
Jiayang Xie ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Drug Resistant ◽  
Excellent Performance ◽  
Persister Cells ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Chimeric Peptide ◽  
Alpha Beta ◽  
Chimeric Polypeptide

An α/β chimeric polypeptide molecular brush shows excellent performance in eradicating established biofilms, persister cells, and clinically isolated multi-drug resistant Gram-positive bacteria.

DNA Binding Ligands with Excellent Antibiotic Potency Against Drug-Resistant Gram-Positive Bacteria.

ChemInform ◽  
2003 ◽  
Vol 34 (6) ◽  
Author(s):  
Roland W. Buerli ◽  
Yigong Ge ◽  
Sarah White ◽  
Eldon E. Baird ◽  
Sofia M. Touami ◽  
...  
Keyword(s):  
Dna Binding ◽  
Drug Resistant ◽  
Gram Positive ◽  
Gram Positive Bacteria

DNA binding ligands targeting drug-resistant Gram-positive bacteria. Part 1: Internal benzimidazole derivatives

2004 ◽  
Vol 14 (5) ◽  
pp. 1253-1257 ◽  
Author(s):  
Roland W. Bürli ◽  
Dustin McMinn ◽  
Jacob A. Kaizerman ◽  
Wenhao Hu ◽  
Yigong Ge ◽  
...  
Keyword(s):  
Dna Binding ◽  
Benzimidazole Derivatives ◽  
Drug Resistant ◽  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals A3, a Scorpion Venom Derived Peptide Analogue with Potent Antimicrobial and Antibiofilm Activity against Clinical Isolates of Multi-Drug Resistant Gram Positive Bacteria

2018 ◽  
Author(s):  
Ammar Almaaytah ◽  
Ahmad Farajallah ◽  
Ahmad Abualhaijaa ◽  
Qosay Al-balas
Keyword(s):  
Host Defense ◽  
Mammalian Cells ◽  
Scorpion Venom ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Gram Positive ◽  
Peptide Analogue ◽  
Gram Positive Bacteria ◽  
Conventional Antibiotics

Current research in the field of antimicrobials is focused on the development of novel antibiotics and antimicrobial agents to counteract the huge dilemma that the human population is mainly facing in regards to the rise of bacterial resistance and biofilm infections. Host Defense peptides (HDPs) are a promising group of molecules for antimicrobial development as they share unique characteristics suitable for antimicrobial activity including their broad spectrum of activity and potency against bacteria. AamAP1 is a novel HDP that was identified through molecular cloning from the venom of the North African scorpion Androctonus amoeruxi. In vitro antimicrobial assays revealed that the peptide displays moderate activity against different strains of Gram-positive and Gram-negative bacteria. Additionally, the peptide proved to be highly hemolytic and displaying significantly high toxicity against mammalian cells. In our study, a novel synthetic peptide analogue named A3 was designed from the naturally occurring scorpion venom host defense peptide. The design strategy depended on modifying the amino acid sequence of the parent peptide in order to increase its net positive charge, percentage helicity and optimize other physico-chemical parameters involved theoretically in HDPs activity. Accordingly, A3 was evaluated for its in vitro antimicrobial and anti-biofilm activity individually and in combination with four different types of conventional antibiotics against clinical isolates of multi-drug resistant (MDR) Gram-positive bacteria. A3 was also evaluated for its cytotoxicity against mammalian cells. A3 displayed potent and selective in vitro antimicrobial activities against a wide range of MDR Gram-positive bacteria. Our results also showed that combining A3 with conventional antibiotics displayed a synergistic mode of action which resulted in decreasing the MIC value for A3 peptide as low as 0.125 µM. These effective concentrations were associated with negligible toxicities on mammalian cells. In conclusion, A3 exhibits enhanced activity and selectivity when compared with the parent natural scorpion venom peptide. The combination of A3 with conventional antibiotics may be pursued as a potential novel treatment strategy against MDR and biofilm forming bacteria.

scholarly journals Re-thinking antimicrobial resistance transmission dynamics: a meta-analysis of cross-sectional studies at referral hospitals in Uganda

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 878
Author(s):  
Gerald Mboowa ◽  
Ivan Sserwadda ◽  
Dickson Aruhomukama
Keyword(s):  
Antimicrobial Resistance ◽  
Transmission Dynamics ◽  
Gram Positive ◽  
Cross Sectional ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Extended Spectrum ◽  
Hospital Environments ◽  
Referral Hospitals ◽  
Cross Sectional Studies

Background: Antimicrobial resistance threatens the achievements of modern medicine as well as the sustainability of effective global public health responses to the threat posed by infectious diseases. Extended-spectrum β-lactamase production in bacteria provides the main mechanism of resistance in gram-negative bacteria, particularly those belonging to the Enterobacteriaceae family as well as gram-positive bacteria. This study hence aimed at providing insights into the potential role of in-patients, their immediate hospital environments, out-patients, and their communities in the transmission of antimicrobial resistance via identifying gram-negative and gram-positive bacteria commonly isolated in samples collected from each of these patients/sites as well as their antimicrobial susceptibility profiles using extended-spectrum β-lactamase production in the same as the basis. Methods: Our study reviewed four cross-sectional studies conducted at national and regional referral hospitals in Uganda. Data on bacterial aetiology and antimicrobial susceptibility testing retrieved from the studies was imported into Microsoft Excel, cleaned, sand then exported to IBM SPSS statistics (version 16) for statistical analysis. The databases used were PubMed and Embase.  Results: We report that; Escherichia coli and Klebsiella pneumoniae are the most prevalent Enterobacteriaceae species in the samples that were collected in the studies reviewed; these species account for the highest proportions of extended-spectrum β-lactamase producers; Staphylococcus aureus is the most prevalent of the gram-positive bacteria isolated from the same samples, and accounts for the highest proportions of extended-spectrum β-lactamase producers in the gram-positive bacteria isolated, and similar Enterobacteriaceae species and gram-positive bacteria, are predominant in samples from in-patients, their immediate hospital environments, and out-patients. Conclusion: The insights provided indicate antimicrobial resistance transmission dynamics be re-thought and more comprehensive studies aimed at investigating the same be done to ascertain the source and transmission routes of antimicrobial-resistant bacteria in clinical settings.

scholarly journals Potent trifluoromethoxy, trifluoromethylsulfonyl, trifluoromethylthio and pentafluorosulfanyl containing (1,3,4-oxadiazol-2-yl)benzamides against drug-resistant Gram-positive bacteria

2020 ◽  
Vol 11 (1) ◽  
pp. 102-110
Author(s):  
George A. Naclerio ◽  
Nader S. Abutaleb ◽  
Kenneth I. Onyedibe ◽  
Mohamed N. Seleem ◽  
Herman O. Sintim
Keyword(s):  
Drug Resistant ◽  
Gram Positive ◽  
Methicillin Resistant ◽  
Gram Positive Bacteria

Switching from bacteriostatic against methicillin-resistant S. aureus to bactericidal via SCF3 or SF5 substitution.

Multifunctional BODIPY for effective inactivation of Gram-positive bacteria and promotion of wound healing

2021 ◽  
Author(s):  
Chaonan Li ◽  
Yite Li ◽  
Qihang Wu ◽  
Tingting Sun ◽  
Zhigang Xie
Keyword(s):  
Wound Healing ◽  
Photodynamic Therapy ◽  
Antimicrobial Resistance ◽  
Infectious Diseases ◽  
Human Health ◽  
Bacterial Infections ◽  
Alternative Therapies ◽  
Gram Positive ◽  
Gram Positive Bacteria

Bacterial infectious diseases and antimicrobial resistance seriously endanger human health, so alternative therapies for bacterial infections are urgently needed. Recently, photodynamic therapy against bacteria has shown great potential because of...

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