Multifunctional antibacterial dental sealants suppress biofilms derived from children at high risk of caries

2020 ◽  
Vol 8 (12) ◽  
pp. 3472-3484 ◽  
Author(s):  
Maria Salem Ibrahim ◽  
Isadora Martini Garcia ◽  
Taissa Vila ◽  
Abdulrahman A. Balhaddad ◽  
Fabrício Mezzomo Collares ◽  
...  
Keyword(s):  
High Risk ◽  
Biofilm Formation ◽  
Dental Sealants ◽  
Dental Sealant ◽  
Bioactive Agents

Dental sealant containing antibacterial and bioactive agents decreased biofilm formation due to the saliva of children at low and high risk of caries.

The Effect of Resin-Based Dental Sealant Containing Vanillin on Biofilm Formation of Candida albicans

2021 ◽  
Vol 904 ◽  
pp. 277-281
Author(s):  
Boonyanit Thaweboon ◽  
Sroisiri Thaweboon
Keyword(s):  
Early Childhood ◽  
Candida Albicans ◽  
Young Children ◽  
Biofilm Formation ◽  
Early Childhood Caries ◽  
Cell Counting ◽  
Dental Sealants ◽  
Dental Sealant ◽  
Candida Biofilm ◽  
Childhood Caries

Early childhood caries (ECC), or baby bottle tooth decay is one of the most common chronic diseases that affect young children at 6 years of age or younger. The disease is resulted from complex interactions between tooth structures and cariogenic microorganisms in dental biofilm after exposure to fermentable carbohydrates through improper feeding practices. This leads to a rapid development of demineralization of teeth shortly after they erupt in the oral cavity. Candida albicans have been reported recently to be associated with the pathogenesis of ECC. One of the general approaches currently used for the prevention of ECC is the application of dental sealant to occlusal (chewing surface) pits and fissures of caries-susceptible teeth. Many types of dental sealants have been extensively used and various antimicrobial agents were introduced to dental sealants to increase their caries preventive effect. The aim of the present study was to evaluate the antimicrobial effect of vanillin incorporated resin-based dental sealant on C. albicans biofilm. Resin-based dental sealants (Clinpro; 3M ESPE, USA) containing different concentrations of vanillin (0%, 0.5%, 1% and 5% w/v) were prepared in 96-well plate. The suspension (107 colony forming unit/mL) of each strain of Candida albicans (ATCC 10231, ATCC 18804 and 2 clinical isolates) was added to saliva-coated sealant resin samples and incubated at 37°C in 5% CO2 atmosphere for 24 h to allow the Candida biofilm formation. The quantity of vital biofilm was determined by WST Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA) at 460 nm. All tests were done in triplicate and repeated three times. Kruskal-Wallis test followed by Dunn’s method were used to account for multiple comparisons. Statistical significance was set at p-value less than 0.05. The results showed that 0.5-5% vanillin-incorporated dental sealant resins showed a significant suppressive effect against all strains of Candida. No significant differences of anti-biofilm effects were found among Candida strains. Approximately 30-50% Candida biofilm reduction was noticed in sealant resins containing vanillin compared with controls without vanillin. In conclusion, the adding of vanillin (0.5-5% w/v) to dental sealant resins is effective in inhibiting biofilm formation of fungus related to early childhood caries, C. albicans. Therefore, the use of dental sealants containing vanillin has the potential to prevent this type of dental caries in young children and biofilm formation by oral Candida. Further clinical studies are required to investigate physical properties of this sealant and clinical evaluation in the patients.

Formation of Cariogenic Bacterial Biofilm on Vanillin-Incorporated Resin-Based Dental Sealant

2021 ◽  
Vol 889 ◽  
pp. 107-111
Author(s):  
Boonyanit Thaweboon ◽  
Sroisiri Thaweboon
Keyword(s):  
Dental Caries ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Antimicrobial Property ◽  
Bacterial Biofilm ◽  
Cell Counting ◽  
Cariogenic Bacteria ◽  
Tooth Structure ◽  
Dental Sealants ◽  
Dental Sealant

Tooth decay or dental caries is an important oral health problem involving people of all age groups. The disease is the outcome of the demineralize process in which aciduric and acidogenic bacteria in a biofilm decompose tooth structure. Dental sealant, a resin material, which is applied on the occlusal pit and fissure surfaces of the teeth as a protective layer has been commonly used to prevent dental caries. However, the microbial effect on food residue is found to be a major cause of microleakage of sealant and secondary caries. Several types of antimicrobial agents were introduced to increase the caries preventive effect of dental sealants. Vanillin, the main component of flavoring agent vanilla, has been found to have antimicrobial property against Gram-positive and Gram-negative bacteria. The objective of this study was to investigate the antimicrobial effect of vanillin-incorporated dental sealant against biofilm formation of cariogenic bacteria. Dental sealant resin samples (Clinpro; 3M ESPE, USA) were prepared in 96-well plate in accordance with the amount of vanillin adding (0%, 0.5%, 1% and 5% vanillin). The cariogenic bacterial suspensions of Streptococcus mutans ATCC 25175 and Lactobacillus casei ATCC 334 were added to saliva-coated samples and incubated at 37°C in 5% CO2 atmosphere for 48 h to allow the biofilm formation. The quantity of vital biofilm was determined by WST Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA) at 460 nm. One-way ANOVA and Tukey’s test were applied to the statistical analysis. A significant inhibitory effect against L. casei biofilm was observed in all vanillin incorporated samples (0.5%, 1% and 5% vanillin) compared with samples without vanillin. The percentage of biofilm reduction was 32-39%. For S. mutans, the suppressive effect was noticed only at >1% vanillin with 18-25% biofilm reduction. In conclusion, the incorporation of vanillin to dental sealants could decrease biofilm formation of cariogenic bacteria (S. mutans and L. casei). The use of dental sealants containing vanillin could be a promising measure to prevent dental caries due to their antibacterial biofilm formation property.

scholarly journals Dental Sealant Composition-Retention Assessment in Young Permanent Molars

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1646
Author(s):  
Alexandrina Muntean ◽  
Codruta Sarosi ◽  
Sorina Sava ◽  
Marioara Moldovan ◽  
Andrei Ilie Condurache ◽  
...  
Keyword(s):  
Preventive Measures ◽  
Public Health Problem ◽  
Tooth Decay ◽  
Permanent Molar ◽  
Maxillary Molars ◽  
Dental Sealants ◽  
Dental Sealant ◽  
Permanent Molars ◽  
First Time ◽  
First Permanent Molars

Tooth decay in children and adolescents remains a public health problem, despite prophylaxis and preventive measures being largely available. The aim of our study was to evaluate the clinical behavior of four dental sealants, related to first permanent molar topography and patient age (when sealant was applied for the first time). We assessed, by means of visual inspection and palpation with a dental probe, a group of 200 children, enrolled corresponding to school age-grade (mean age of 7 years at baseline) and randomly divided according to the material used as dental sealant (Admira seal©, Embrace Wet Bond©, Fotoseal©, GC Fuji Triaje©) in 4 groups (n = 50). Sealant clinical evaluation was made at 6-, 12-, 18-month intervals for dental material retention assessment. At 6 months, the sealant detached the most from 3.6 molars, and the material used was Fotoseal© (27.6%). At 12 months, Fotoseal© (48.3%) and GC Fuji Triaje© (41.4%) from 3.6 molars express detachment. At 18 months, 4.6. molars sealed with Admira Seal© (25.7%) and Embrace Wet Bond© (28.6%) lost the sealant. We noticed less detachment in maxillary molars and if sealant was applied around 7 years of age. In conclusion, sealant application on first permanent molars must be encouraged and practitioners can choose between various materials available.

Antibacterial efficacy of R-type pyocins against Pseudomonas aeruginosa on biofilms and in a murine model of acute lung infection

2020 ◽  
Author(s):  
Mar Redero ◽  
Javier Aznar ◽  
Ana I Prieto
Keyword(s):  
Pseudomonas Aeruginosa ◽  
High Risk ◽  
Murine Model ◽  
Biofilm Formation ◽  
Bacterial Load ◽  
Preventive Treatment ◽  
Lung Infection ◽  
Before And After ◽  
Treatment Alternatives ◽  
Potential Use

Abstract Background The appearance of MDR strains and the development of biofilms make Pseudomonas aeruginosa infections a therapeutic challenge. To overcome this scenario, bacteriocins have been proposed as a potential adjuvant or alternative to antibiotic treatment. Objectives To study the activity of R-pyocins on biofilms and in a murine model of pneumonia using a high-risk clone of P. aeruginosa. Methods The activity of R-pyocins on P. aeruginosa biofilms was tested on bacteria attached to a silicone surface, before and after biofilm formation. The effectiveness of R1-pyocin was studied in a murine model of pneumonia using ST175, a high-risk clone of P. aeruginosa. Results R-pyocins attacked adherent bacteria, preventing biofilm formation, and penetrated into the biofilm, killing P. aeruginosa within it, resulting in a dramatic reduction in bacterial load. R1-pyocin was active in a murine model of P. aeruginosa lung infection, administered before infection as a preventive treatment, and in acute pneumonia, with efficiency higher than standard colistin treatment. In addition, this work is the first to describe histopathological lung changes after administration of R-pyocins, contributing to the resolution of P. aeruginosa pneumonia in a murine model. Conclusions This work highlights the potential use of the R-pyocins as therapeutic agents, alone or as adjuvants, due to its effectiveness on biofilms and in a murine model of pneumonia using ST175, a high-risk clone of P. aeruginosa. It may thus be feasible to consider R-pyocins as a possible therapeutic alternative in XDR infections, where treatment alternatives are limited.

scholarly journals Biological Markers of Pseudomonas aeruginosa Epidemic High-Risk Clones

2013 ◽  
Vol 57 (11) ◽  
pp. 5527-5535 ◽  
Author(s):  
Xavier Mulet ◽  
Gabriel Cabot ◽  
Alain A. Ocampo-Sosa ◽  
M. Angeles Domínguez ◽  
Laura Zamorano ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
High Risk ◽  
Multicenter Study ◽  
Biological Markers ◽  
Biofilm Formation ◽  
Specific Treatment ◽  
Spontaneous Mutant ◽  
Biological Parameters ◽  
Content Type

ABSTRACTA limited number ofPseudomonas aeruginosagenotypes (mainly ST-111, ST-175, and ST-235), known as high-risk clones, are responsible for epidemics of nosocomial infections by multidrug-resistant (MDR) or extensively drug-resistant (XDR) strains worldwide. We explored the potential biological parameters that may explain the success of these clones. A total of 20 isolates from each of 4 resistance groups (XDR, MDR, ModR [resistant to 1 or 2 classes], and MultiS [susceptible to all antipseudomonals]), recovered from a multicenter study ofP. aeruginosabloodstream infections performed in 10 Spanish hospitals, were analyzed. A further set of 20 XDR isolates belonging to epidemic high-risk clones (ST-175 [n= 6], ST-111 [n= 7], and ST-235 [n= 7]) recovered from different geographical locations was also studied. When unknown, genotypes were documented through multilocus sequence typing. The biological parameters evaluated included twitching, swimming, and swarming motility, biofilm formation, production of pyoverdine and pyocyanin, spontaneous mutant frequencies, and thein vitrocompetition index (CI) obtained with a flow cytometry assay. All 20 (100%) XDR, 8 (40%) MDR, and 1 (5%) ModR bloodstream isolate from the multicenter study belonged to high-risk clones. No significant differences were observed between clonally diverse ModR and MultiS isolates for any of the parameters. In contrast, MDR/XDR high-risk clones showed significantly increased biofilm formation and mutant frequencies but significantly reduced motility (twitching, swimming, and swarming), production of pyoverdine and pyocyanin, and fitness. The defined biological markers of high-risk clones, which resemble those resulting from adaptation to chronic infections, could be useful for the design of specific treatment and infection control strategies.

Clinical Trial Concerning the Effectiveness of Two Types Dental Sealants in Children with Bruxism

2020 ◽  
Vol 57 (1) ◽  
pp. 191-196
Author(s):  
Edwin Sever Bechir ◽  
Mircea Suciu ◽  
Farah Bechir ◽  
Florin-Daniel Berneanu ◽  
Anamaria Bechir ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Preventive Dentistry ◽  
Dental Sealants ◽  
Child Patients ◽  
Dental Sealant ◽  
Unfilled Resin

Dental sealants are important components of preventive dentistry. The aim of this clinical trial was to compare the effectiveness of an unfilled resin-based sealant and a carbomer-based dental sealant. 42 child patients with completely erupted inferior first molars and with diagnosed bruxism were included in the study. At 2 weeks, respectively 6, 12 and 18 months were evaluated the effectiveness of sealants after marginal deterioration, retention and the presence of caries. The results of the trial showed that unfilled resin-based sealant presented fewer modifications.

scholarly journals Strategies to Reduce Biofilm Formation in PEEK Materials Applied to Implant Dentistry—A Comprehensive Review

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 609
Author(s):  
Renata Scheeren Brum ◽  
Luiza Gomes Labes ◽  
Cláudia Ângela Maziero Volpato ◽  
César Augusto Magalhães Benfatti ◽  
Andrea de Lima Pimenta
Keyword(s):  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Antimicrobial Properties ◽  
Comprehensive Review ◽  
Polyether Ether Ketone ◽  
Implant Dentistry ◽  
Bioactive Agents ◽  
Short Time ◽  
And Staphylococcus Aureus ◽  
New Strategies

Polyether-ether-ketone (PEEK) has emerged in Implant Dentistry with a series of short-time applications and as a promising material to substitute definitive dental implants. Several strategies have been investigated to diminish biofilm formation on the PEEK surface aiming to decrease the possibility of related infections. Therefore, a comprehensive review was carried out in order to compare PEEK with materials widely used nowadays in Implant Dentistry, such as titanium and zirconia, placing emphasis on studies investigating its ability to grant or prevent biofilm formation. Most studies failed to reveal significant antimicrobial activity in pure PEEK, while several studies described new strategies to reduce biofilm formation and bacterial colonization on this material. Those include the PEEK sulfonation process, incorporation of therapeutic and bioactive agents in PEEK matrix or on PEEK surface, PEEK coatings and incorporation of reinforcement agents, in order to produce nanocomposites or blends. The two most analyzed surface properties were contact angle and roughness, while the most studied bacteria were Escherichia coli and Staphylococcus aureus. Despite PEEK’s susceptibility to biofilm formation, a great number of strategies discussed in this study were able to improve its antibiofilm and antimicrobial properties.

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