Spatial immobilization of endogenous growth factors to control vascularization in bone tissue engineering

2020 ◽  
Vol 8 (9) ◽  
pp. 2577-2589 ◽  
Author(s):  
Marta R. Casanova ◽  
Catarina Oliveira ◽  
Emanuel M. Fernandes ◽  
Rui L. Reis ◽  
Tiago H. Silva ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Endogenous Growth ◽  
Culture Conditions ◽  
Parallel Pattern

An engineered biofunctional system comprises endogenous BMP-2 and VEGF bound in a parallel pattern. It successfully enabled obtaining the spatial osteogenic and angiogenic differentiation of human hBM-MSCs under basal culture conditions.

scholarly journals Scaffolds for Growth Factor Delivery as Applied to Bone Tissue Engineering

2012 ◽  
Vol 2012 ◽  
pp. 1-25 ◽  
Author(s):  
Keith A. Blackwood ◽  
Nathalie Bock ◽  
Tim R. Dargaville ◽  
Maria Ann Woodruff
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Donor Site ◽  
Structural Features ◽  
Donor Site Morbidity ◽  
Tissue Type ◽  
Specific Cell ◽  
Growth Factor Delivery

There remains a substantial shortfall in the treatment of severe skeletal injuries. The current gold standard of autologous bone grafting from the same patient has many undesirable side effects associated such as donor site morbidity. Tissue engineering seeks to offer a solution to this problem. The primary requirements for tissue-engineered scaffolds have already been well established, and many materials, such as polyesters, present themselves as potential candidates for bone defects; they have comparable structural features, but they often lack the required osteoconductivity to promote adequate bone regeneration. By combining these materials with biological growth factors, which promote the infiltration of cells into the scaffold as well as the differentiation into the specific cell and tissue type, it is possible to increase the formation of new bone. However due to the cost and potential complications associated with growth factors, controlling the rate of release is an important design consideration when developing new bone tissue engineering strategies. This paper will cover recent research in the area of encapsulation and release of growth factors within a variety of different polymeric scaffolds.

Spatial Immobilization of Autologous Growth Factors to Control Vascularization in Bone Tissue Engineering

2019 ◽  
Author(s):  
Marta R. Casanova ◽  
Catarina Oliveira ◽  
Emanuel M. Fernandes ◽  
Rui L. Reis ◽  
Tiago H. Silva ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals The Role of Growth Factors in Bioactive Coatings

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1083
Author(s):  
Dragana Bjelić ◽  
Matjaž Finšgar
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Healing Process ◽  
Therapeutic Agents ◽  
Ageing Population ◽  
Bioactive Coatings ◽  
Bioactive Coating ◽  
Advantages And Disadvantages

With increasing obesity and an ageing population, health complications are also on the rise, such as the need to replace a joint with an artificial one. In both humans and animals, the integration of the implant is crucial, and bioactive coatings play an important role in bone tissue engineering. Since bone tissue engineering is about designing an implant that maximally mimics natural bone and is accepted by the tissue, the search for optimal materials and therapeutic agents and their concentrations is increasing. The incorporation of growth factors (GFs) in a bioactive coating represents a novel approach in bone tissue engineering, in which osteoinduction is enhanced in order to create the optimal conditions for the bone healing process, which crucially affects implant fixation. For the application of GFs in coatings and their implementation in clinical practice, factors such as the choice of one or more GFs, their concentration, the coating material, the method of incorporation, and the implant material must be considered to achieve the desired controlled release. Therefore, the avoidance of revision surgery also depends on the success of the design of the most appropriate bioactive coating. This overview considers the integration of the most common GFs that have been investigated in in vitro and in vivo studies, as well as in human clinical trials, with the aim of applying them in bioactive coatings. An overview of the main therapeutic agents that can stimulate cells to express the GFs necessary for bone tissue development is also provided. The main objective is to present the advantages and disadvantages of the GFs that have shown promise for inclusion in bioactive coatings according to the results of numerous studies.

6.2 Bone Tissue Engineering: Growth Factors and Cytokines ☆

2017 ◽  
pp. 20-53
Author(s):  
J.O. Hollinger ◽  
P. Alvarez-Urena ◽  
P. Ducheyne ◽  
A. Srinivasan ◽  
J. Baskin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals Microbial-Derived Polyhydroxyalkanoate-Based Scaffolds for Bone Tissue Engineering: Biosynthesis, Properties, and Perspectives

2021 ◽  
Vol 9 ◽  
Author(s):  
Jian Li ◽  
Xu Zhang ◽  
Anjaneyulu Udduttula ◽  
Zhi Shan Fan ◽  
Jian Hai Chen ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biomedical Applications ◽  
Large Scale ◽  
Lower Cost ◽  
Culture Conditions ◽  
Manufacturing Technologies ◽  
Insight Into

Polyhydroxyalkanoates (PHAs) are a class of structurally diverse natural biopolyesters, synthesized by various microbes under unbalanced culture conditions. PHAs as biomedical materials have been fabricated in various forms to apply to tissue engineering for the past years due to their excellent biodegradability, inherent biocompatibility, modifiable mechanical properties, and thermo-processability. However, there remain some bottlenecks in terms of PHA production on a large scale, the purification process, mechanical properties, and biodegradability of PHA, which need to be further resolved. Therefore, scientists are making great efforts via synthetic biology and metabolic engineering tools to improve the properties and the product yields of PHA at a lower cost for the development of various PHA-based scaffold fabrication technologies to widen biomedical applications, especially in bone tissue engineering. This review aims to outline the biosynthesis, structures, properties, and the bone tissue engineering applications of PHA scaffolds with different manufacturing technologies. The latest advances will provide an insight into future outlooks in PHA-based scaffolds for bone tissue engineering.

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