Fluorescent Detection of the Target Protein via a Molecularly Imprinted Hydrogel

2021 ◽  
Author(s):  
Takuya Kubo ◽  
Naoki Watanabe ◽  
Chenchen Liu ◽  
Seiji Ikari ◽  
Eisuke Kanao ◽  
...  
Keyword(s):  
Immune Reaction ◽  
Fluorescent Labeling ◽  
Target Protein ◽  
Fluorescent Detection ◽  
Molecularly Imprinted ◽  
P Proteins ◽  
Denaturation Of Proteins

Proteins are typically separated by immune-reaction, such as enzyme-linked immuno sorbent assay, and detected by certain fluorescent labeling. They have usually potential of complicated procedures, denaturation of proteins by labeling,...

scholarly journals Chemodosimeter-based fluorescent detection of l-cysteine after extracted by molecularly imprinted polymers

Talanta ◽  
2014 ◽  
Vol 120 ◽  
pp. 297-303 ◽  
Author(s):  
Xiaoqiang Cai ◽  
Jinhua Li ◽  
Zhong Zhang ◽  
Gang Wang ◽  
Xingliang Song ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Fluorescent Detection ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Gas-stimuli-responsive molecularly imprinted polymer particles with switchable affinity for target protein

2018 ◽  
Vol 54 (20) ◽  
pp. 2538-2541 ◽  
Author(s):  
Yukiya Kitayama ◽  
Manabu Isomura
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Target Protein ◽  
Stimuli Responsive ◽  
Polymer Particles ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

Molecularly imprinted polymer particles bearing gas-responsive property was successfully prepared using functional initiator.

Clarification techniques

2001 ◽  
Author(s):  
Ian Reed ◽  
Duncan Mackay
Keyword(s):  
Fermentation Broth ◽  
Complex Mixture ◽  
Particulate Material ◽  
Target Protein ◽  
Target Product ◽  
Immiscible Liquids ◽  
Practical Advice ◽  
Dissolved Species ◽  
P Proteins ◽  
Scale Of Operation

Proteins can be produced by a number of different routes such as fermentation, tissue culture, and by extraction from plasma or plants. Whatever route is chosen, the raw protein-bearing stream is likely to be a complex mixture containing both dissolved species and particulate material. The target protein will be present at very low concentration and with a host of contaminants such as cells or cell debris, DNA, proteins and polysaccharides, and a large quantity of water. Such a mixture is very difficult to treat using the highly selective processes that are required to obtain the target product at high purity since the presence of particulate material impairs their function. The first challenge of protein purification is therefore to convert the complex fermentation broth which is a mixture of dissolved and suspended solids into a form that is amenable to further purification. Although there is much interest in direct recovery of protein from such materials, the most frequent first step currently is to clarify the raw protein source to remove suspended matter. It is then possible to use a range of highly selective techniques to purify the target protein. There are a number of clarification techniques that can be adopted and the choice of which to use depends on both the source of raw feed and the scale of operation. There are two main classes of process; sedimentation and filtration. Sedimentation can be carried out under normal gravity conditions or, as is almost always the case for biological streams, using a centrifuge. Filtration can be performed using either conventional filter media or using membrane filters for removal of finer particles. The aim of this chapter is to describe these methods, and their underlying principles, the advantages of each are discussed, and examples of equipment are presented. Practical advice is presented on how and when to use each technique. Sedimentation processes operate primarily on the basis of density differences between the various components of a mixture. They are most commonly applied to suspensions of solid in liquid, but also to disengage immiscible liquids. If there is no density difference between particulates and the suspending medium, sedimentation cannot occur.

Fluorescent molecularly imprinted membranes as biosensor for the detection of target protein

2018 ◽  
Vol 254 ◽  
pp. 1078-1086 ◽  
Author(s):  
Xin Zhang ◽  
Shu Yang ◽  
Rui Jiang ◽  
Liquan Sun ◽  
Siping Pang ◽  
...  
Keyword(s):  
Target Protein ◽  
Molecularly Imprinted

Composite Material Based on Carbon Dots and Molecularly Imprinted Polymers: A Facile Probe for Fluorescent Detection of 4-Nitrophenol

NANO ◽  
2020 ◽  
Vol 15 (08) ◽  
pp. 2050105
Author(s):  
Min Wang ◽  
Manjie Gao ◽  
Linlin Deng ◽  
Xun Kang ◽  
Lijuan Yang ◽  
...  
Keyword(s):  
Composite Material ◽  
Molecularly Imprinted Polymers ◽  
Carbon Dots ◽  
Limit Of Detection ◽  
Sol Gel ◽  
Synthesis Method ◽  
Fluorescent Detection ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Selective Determination

A new fluorescence composite material for the sensitive and selective determination of 4-nitrophenol (4-NP) was developed based on molecularly imprinted polymers (MIPs) incorporated with carbon dots (CDs). First, fluorescent CDs with a high quantum yield (QY) of 51.8% were prepared by hydrothermal synthesis method by using anhydrous citric acid as carbon source and AEAPMS as surface modifier. Then, CDs were fabricated with MIPs (CDs@MIPs) by sol–gel method using 4-NP as template, (3-aminopropyl) triethoxysilane (APTES) as functional monomer, tetraethoxysilane (TEOS) as cross-linker and CDs as signal sources, respectively. The CDs@MIPs exhibited strong fluorescence property and high selectivity to 4-NP as it incorporated merits of CDs and MIPs. Under optimized conditions, the relative fluorescence intensity of CDs@MIPs decreased linearly with the concentration of 4-NP from 0.025[Formula: see text][Formula: see text]g[Formula: see text]mL[Formula: see text] to 5[Formula: see text][Formula: see text]g[Formula: see text]mL[Formula: see text]. The limit of detection (LOD) of 4-NP was 5[Formula: see text]ng[Formula: see text]mL[Formula: see text] (35[Formula: see text]nM). Specificity and selectivity experiments showed that CDs@MIPs can selectively detect 4-NP with rare interference of other competitive analogs and metal ions. Finally, CDs@MIPs was successfully used to detect 4-NP in river water samples with the recoveries ranging from 94.0% to 103.4%. The results demonstrated that the prepared CDs@MIPs can be applied to the selective and sensitive detection of trace 4-NP in real samples.

scholarly journals An eco-friendly imprinted polymer based on graphene quantum dots for fluorescent detection of p-nitroaniline

RSC Advances ◽  
2019 ◽  
Vol 9 (71) ◽  
pp. 41383-41391 ◽  
Author(s):  
Lei Cai ◽  
Zhaohui Zhang ◽  
Haimei Xiao ◽  
Shan Chen ◽  
Jinli Fu
Keyword(s):  
Quantum Dots ◽  
Sol Gel ◽  
Graphene Quantum Dots ◽  
Fluorescent Detection ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Selective Determination ◽  
Highly Sensitive ◽  
Polymer Anchored

An eco-friendly fluorescent molecularly imprinted polymer anchored on the surface of graphene quantum dots (GQDs@MIP) was developed with an efficient sol–gel polymerization for highly sensitive and selective determination of p-nitroaniline (p-NA).

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