A strategy based on isocratic and linear-gradient High-Speed Counter-Current Chromatography for comprehensive separation of platycosides from Platycodi Radix

2021 ◽  
Author(s):  
Jin-Qian Yu ◽  
Xiangwei Chang ◽  
Huasheng Peng ◽  
Xiao Wang ◽  
Jutao Wang ◽  
...  
Keyword(s):  
Efficient Method ◽  
High Speed ◽  
Pharmacological Activities ◽  
Linear Gradient ◽  
Active Constituents ◽  
Platycodi Radix ◽  
Counter Current

Platycosides, the generally recognized main active constituents of Platycodi Radix, have been studied extensively for its wide pharmacological activities. Herein, we have successfully developed an efficient method for enrichment and...

HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent

2020 ◽  
Author(s):  
Xue Yang ◽  
Yongling Liu ◽  
Tao Chen ◽  
Nana Wang ◽  
Hongmei Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Ethyl Acetate ◽  
Dimethyl Sulfoxide ◽  
Efficient Method ◽  
Crude Extract ◽  
High Speed ◽  
Glacial Acetic Acid ◽  
Butyl Alcohol ◽  
Preparative Hplc ◽  
Counter Current

Abstract Separation of natural compounds directly from the crude extract is a challenging work for traditional column chromatography. In the present study, an efficient method for separation of three main compounds from the crude extract of Dracocephalum tanguticum has been successfully established by high-speed counter-current chromatography (HSCCC). The crude extract was directly introduced into HSCCC by using dimethyl sulfoxide as cosolvent. Ethyl acetate/n-butyl alcohol/0.3% glacial acetic acid (4: 1: 5, v/v) system was used and three target compounds with purity higher than 80% were obtained. Preparative HPLC was used for further purification and three target compounds with purity higher than 98% were obtained. The compounds were identified as chlorogenic acid, pedaliin and pedaliin-6″-acetate.

Use of O-carboxymethyl chitosan in high-speed counter-current chromatography: a novel additive for a biphasic solvent system

2014 ◽  
Vol 38 (3) ◽  
pp. 1150-1157 ◽  
Author(s):  
Yanjuan Liu ◽  
Gaohong Wang ◽  
Xinyi Huang ◽  
Yongfeng Liu ◽  
Duolong Di
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Carboxymethyl Chitosan ◽  
Separation And Purification ◽  
Active Constituents ◽  
System P ◽  
Natural Plants ◽  
Counter Current

A novel additive,O-carboxymethyl chitosan, for high-speed counter-current chromatography was found and evaluated for the separation and purification of active constituents from natural plants.

scholarly journals Preparative separation of mangiferin glycosides by high speed counter current chromatography and comparison of their antioxidant and antitumor activities

RSC Advances ◽  
2020 ◽  
Vol 10 (43) ◽  
pp. 25780-25785
Author(s):  
Tingting Xu ◽  
Xueming Wu
Keyword(s):  
Efficient Method ◽  
High Speed ◽  
Preparative Separation ◽  
Antitumor Activities ◽  
Counter Current ◽  
Enzymatic Glycosylation

High-speed counter-current chromatography is an efficient method for separation of mangiferin glycosides from enzymatic glycosylation.

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