scholarly journals Evaluation of extraction methods for pharmacologically active compounds from anticonvulsant traditional Chinese medicines: Gou Teng, Tian Ma, Jiang Can using DART-TOF-MS

2021 ◽  
Vol 13 (7) ◽  
pp. 884-893
Author(s):  
Kimberly N. Karin ◽  
Justin L. Poklis ◽  
Michelle R. Peace
Keyword(s):  
Extraction Methods ◽  
Rapid Analysis ◽  
Traditional Chinese Medicines ◽  
Microwave Assisted Extraction ◽  
Chinese Medicines ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Pharmacologically Active ◽  
Pharmacologically Active Compounds ◽  
Tof Ms

With the popularity of natural products, rapid analysis of pharmacologically active analytes from natural matrices is critical for public health. Microwave-assisted extraction with DART-MS provides efficient analysis of analytes from herbal matrices.

scholarly journals Pressurized Liquid Extraction and Microwave-Assisted Extraction in the Determination of Organochlorine Pesticides in Fish Muscle Samples

2008 ◽  
Vol 91 (1) ◽  
pp. 174-180 ◽  
Author(s):  
Mercedes Barriada-Pereira ◽  
Iván Iglesias-García ◽  
María J Gonzlez-Castro ◽  
Soledad Muniategui-Lorenzo ◽  
Purificación López-Maha ◽  
...  
Keyword(s):  
Organochlorine Pesticides ◽  
Fish Muscle ◽  
Extraction Methods ◽  
Liquid Extraction ◽  
Pressurized Liquid Extraction ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Relative Standard

Abstract This paper describes a comparative study of 2 extraction methods, pressurized liquid extraction (PLE) and microwave-assisted extraction (MAE), for the determination of organochlorine pesticides (OCPs) in fish muscle samples. In both cases, samples were extracted with hexaneacetone (50 + 50), and the extracts were purified by solid-phase extraction using a carbon cartridge as the adsorbent. Pesticides were eluted with hexaneethyl acetate (80 + 20) and determined by gas chromatography with electron-capture detection. Both methods demonstrated good linearity over the range studied (0.0050.100 g/mL). Detection limits ranged from 0.029 to 0.295 mg/kg for PLE and from 0.003 to 0.054 mg/kg for MAE. For most of the pesticides, analytical recoveries with both methods were between 80 and 120, and the relative standard deviations were <10. The proposed methods were shown to be powerful techniques for the extraction of OCPs from fish muscle samples. Although good recovery rates were obtained with both extraction methods, MAE provided advantages with regard to sample handling, cost, analysis time, and solvent consumption. Acceptable validation parameters were obtained although MAE was shown to be more sensitive than PLE.

scholarly journals Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 456 ◽  
Author(s):  
Ding-Tao Wu ◽  
Wen Liu ◽  
Mei-Lin Xian ◽  
Gang Du ◽  
Xin Liu ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Antioxidant Activities ◽  
Extraction Methods ◽  
Water Extraction ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Hovenia Dulcis

Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from Hovenia dulcis. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry.

scholarly journals Microwave-Assisted Extraction of Polyphenol Content from Leaves of Tristaniopsis merguensis Griff.

2020 ◽  
Vol 19 (2) ◽  
pp. 110 ◽  
Author(s):  
Robby Gus Mahardika ◽  
Occa Roanisca
Keyword(s):  
Reaction System ◽  
Extraction Methods ◽  
Acetone Extract ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Polyphenol Content ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Long Time ◽  
Gallic Acid Equivalent

Tristaniopsis merguensis Griff. is a species of the Myrtaceae family and has been widely used by people of Bangka Belitung as a traditional medicine to reduce cholesterol, gastric pains, and improve cardiac performance. Extraction methods are the crucial efficacy of herbal medicine. The conventional method, like maceration, takes a long time. In this study, the leaves of Tristaniopsis merguensis were extracted using Microwave-Assisted Extraction (MAE) to reduce extraction time. The extraction using MARS (Microwave Accelerated Reaction System) 6 by CEM Corporation with time variation times of 5, 10, 15, 30 min with temperature of 60, 80, 100oC at 1200 W. The yield using acetone extraction of Tristaniopsis merguensis leaves increases with time and temperature. The extraction dependent on solvent extraction, polar solvent like ethanol, and methanol were higher than semi-polar solvents like acetone and ethyl acetate. The polyphenol content of acetone extract using MAE (10 min, 80oC) was found to be 234.67 mg Gallic Acid Equivalent per gram (GAE/g); it was higher than acetone extract using maceration. The phytochemical results show there are no difference in the active compound using MAE and maceration, i.e. alkaloids, tannin, and flavonoids. Yield extraction, time, and phytochemical results of MAE are more favorable than a maceration.

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