An “on–off–on” selective fluorescent probe based on nitrogen and sulfur co-doped carbon dots for detecting Cu2+ and GSH in living cells

2020 ◽  
Vol 12 (42) ◽  
pp. 5110-5119
Author(s):  
Shanshan Wei ◽  
Tinghua Li ◽  
Xinyu Zhang ◽  
Hongyuan Zhang ◽  
Chunzhu Jiang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Living Cells ◽  
Doped Carbon Dots ◽  
Co Doped

Nitrogen and sulfur co-doped carbon dots (N,S-CDs) as a simple “on–off–on” fluorescent probe were applied for quickly detecting Cu2+ ions and GSH in living cells.

Nitrogen and Sulfur Co-doped Fluorescent Carbon Dots for the Detection of Morin and Cell Imaging

2018 ◽  
Vol 15 (1) ◽  
pp. 47-55
Author(s):  
Xuebing Li ◽  
Haifen Yang ◽  
Ning Wang ◽  
Tijian Sun ◽  
Wei Bian ◽  
...  
Keyword(s):  
Microwave Heating ◽  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Water Soluble ◽  
Heating Process ◽  
X Ray ◽  
Doped Carbon Dots ◽  
Co Doped

Background: Morin has many pharmacological functions including antioxidant, anticancer, anti-inflammatory, and antibacterial effects. It is commonly used in the treatment of antiviral infection, gastropathy, coronary heart disease and hepatitis B in clinic. However, researches have shown that morin is likely to show prooxidative effects on the cells when the amount of treatment is at high dose, leading to the decrease of intracellular ATP levels and the increase of necrosis process. Therefore, it is necessary to determine the concentration of morin in biologic samples. Method: Novel water-soluble and green nitrogen and sulfur co-doped carbon dots (NSCDs) were prepared by a microwave heating process with citric acid and L-cysteine. The fluorescence spectra were collected at an excitation wavelength of 350 nm when solutions of NSCDs were mixed with various concentrations of morin. Results: The as-prepared NSCDs were characterized by transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The fluorescence intensity of NSCDs decreased significantly with the increase of morin concentration. The fluorescence intensity of NSCDs displayed a linear response to morin in the concentration 0.10-30 μM with a low detection limit of 56 nM. The proposed fluorescent probe was applied to analysis of morin in human body fluids with recoveries of 98.0-102%. Conclusion: NSCDs were prepared by a microwave heating process. The present analytical method is sensitive to morin. The quenching process between NSCDs and morin is attributed to the static quenching. In addition, the cellular toxicity on HeLa cells indicated that the as-prepared NSCDs fluorescent probe does not show obvious cytotoxicity in cell imaging. Our proposed method possibly opens up a rapid and nontoxic way for preparing heteroatom doped carbon dots with a broad application prospect.

Label-free fluorescence assay based on near-infrared B,N-doped carbon dots as a fluorescent probe for the detection of sialic acid

2020 ◽  
Vol 44 (6) ◽  
pp. 2350-2356 ◽  
Author(s):  
Nan Wang ◽  
Mengke Wang ◽  
Yang Yu ◽  
Guojian Yang ◽  
Xingguang Su
Keyword(s):  
Sialic Acid ◽  
Fluorescent Probe ◽  
Carbon Dots ◽  
Near Infrared ◽  
Fluorescence Assay ◽  
Label Free ◽  
Activity Detection ◽  
Doped Carbon Dots ◽  
Co Doped

A simple and sensitive sensing strategy for sialic acid activity detection on the basis of novel near-infrared B,N co-doped carbon dots was constructed.

scholarly journals Selective and sensitive detection of cinnamaldehyde by nitrogen and sulphur co-doped carbon dots: a detailed systematic study

RSC Advances ◽  
2018 ◽  
Vol 8 (74) ◽  
pp. 42361-42373 ◽  
Author(s):  
Suraj Konar ◽  
Dipanjan Samanta ◽  
Subhajit Mandal ◽  
Subhayan Das ◽  
Madhusudan Kr Mahto ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Systematic Study ◽  
Carbon Dots ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Doped Carbon Dots ◽  
Co Doped

Nitrogen and sulfur co-doped carbon dots (NSCDs) have been used as a fluorescent probe for the sensitive and selective detection of clinically important organic aldehyde cinnamaldehyde.

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