SERS-based magnetic immunoassay for simultaneous detection of cTnI and H-FABP using core–shell nanotags

2020 ◽  
Vol 12 (45) ◽  
pp. 5442-5449
Author(s):  
Cunming Hu ◽  
Li Ma ◽  
Ming Guan ◽  
Fang Mi ◽  
Fei Peng ◽  
...  
Keyword(s):  
Magnetic Beads ◽  
Reduction Method ◽  
Direct Reduction ◽  
Simultaneous Detection ◽  
Quantitative Detection ◽  
Core Shell

Au-4MBA@Ag and Au-XP013@Ag were successfully synthesized by a direct reduction method. After coupling the antibody, the simultaneous quantitative detection of cTnI and H-ABP was achieved by detecting the signal on the magnetic beads.

scholarly journals A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens

2021 ◽  
Vol 18 (9) ◽  
pp. 4574
Author(s):  
Kai Chen ◽  
Biao Ma ◽  
Jiali Li ◽  
Erjing Chen ◽  
Ying Xu ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Quantitative Detection ◽  
Bacterial Strains ◽  
Food Borne Pathogens ◽  
Food Borne

Food-borne pathogens have become an important public threat to human health. There are many kinds of pathogenic bacteria in food consumed daily. A rapid and sensitive testing method for multiple food-borne pathogens is essential. Europium nanoparticles (EuNPs) are used as fluorescent probes in lateral flow immunoassays (LFIAs) to improve sensitivity. Here, recombinase polymerase amplification (RPA) combined with fluorescent LFIA was established for the simultaneous and quantitative detection of Listeria monocytogenes, Vibrio parahaemolyticus, and Escherichia coliO157:H7. In this work, the entire experimental process could be completed in 20 min at 37 °C. The limits of detection (LODs) of EuNP-based LFIA–RPA were 9.0 colony-forming units (CFU)/mL for Listeria monocytogenes, 7.0 CFU/mL for Vibrio parahaemolyticus, and 4.0 CFU/mL for Escherichia coliO157:H7. No cross-reaction could be observed in 22 bacterial strains. The fluorescent LFIA–RPA assay exhibits high sensitivity and good specificity. Moreover, the average recovery of the three food-borne pathogens spiked in food samples was 90.9–114.2%. The experiments indicate the accuracy and reliability of the multiple fluorescent test strips. Our developed EuNP-based LFIA–RPA assay is a promising analytical tool for the rapid and simultaneous detection of multiple low concentrations of food-borne pathogens.

Research on Model of Solid-Solid Reaction and Experiment

2012 ◽  
Vol 236-237 ◽  
pp. 751-754
Author(s):  
Ding Guo Zhao ◽  
Shu Huan Wang ◽  
Xiao Jie Cui ◽  
He Jun Zhang
Keyword(s):  
Low Temperature ◽  
Boron Oxide ◽  
Reduction Method ◽  
Direct Reduction ◽  
Solid Model ◽  
Reaction Equation ◽  
Solid Reaction ◽  
Average Value

In the paper, the reaction of solid-solid model was researched and we confirmed the reaction equation. The experiments in the lab shown that the t1/2 is 11.8min in the ferrosilicon reduction boron anhydride reaction at low temperature. The yield of boron is higher by direct reduction method smelting. In the boron oxide reducing experiment, the yield minimum is 76%, the maximum is 82%, and the average value is about 79%, which provides favorable foundation conditions for smelting amorphous mother alloy.

Simultaneous Enrichment and On-line Detection of Low-Concentration Copper, Cobalt, and Nickel Ions in Water by Near-Infrared Diffuse Reflectance Spectroscopy Combined with Chemometrics

2017 ◽  
Vol 100 (2) ◽  
pp. 560-565 ◽  
Author(s):  
Jibran Iqbal ◽  
Yiping Du ◽  
Fares Howari ◽  
Mahmoud Bataineh ◽  
Nawshad Muhammad ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Fluidized Bed ◽  
Relative Error ◽  
Heavy Metal Ions ◽  
Diffuse Reflectance ◽  
Diffuse Reflectance Spectroscopy ◽  
Simultaneous Detection ◽  
Quantitative Detection ◽  
On Line

Abstract Sensitive detection of heavy metal ions in water is of great importance considering the effects that heavy metals have on public health. A developed fluidized bed enrichment technique was used to concentrate and detect low concentrations of Cu2+, Co2+, and Ni2+ in water samples by near-IR diffuse reflectance (NIDR) spectroscopy (NIDRS) directly without using any chemicals or reagents. The NIDR spectraof adsorbent were measured on-line, and quantitative detection was achieved by applying a built partial least-squares chemometric model. Sensitivity and accuracy was improved significantly because large-volume mixture solutions were used in the enrichment process. Root mean square error of cross-validation values for Cu2+, Co2+, and Ni2+ were 0.29, 0.41, and 0.35 μg/mL, respectively, with mean relative error values in the acceptable range of 6.56–10.27%. This study confirms the potential application of fluidized bed enrichment combined with NIDRS and chemometrics for the simultaneous detection of trace heavy metal ions in water, with low relative error.

Gradual growth of gold nanoseeds on silica for SiO2@gold homogeneous nano core/shell applications by the chemical reduction method

2013 ◽  
Vol 87 (2) ◽  
pp. 025802 ◽  
Author(s):  
H Rezvani Nikabadi ◽  
N Shahtahmasebi ◽  
M Rezaee Rokn-Abadi ◽  
M M Bagheri Mohagheghi ◽  
E K Goharshadi
Keyword(s):  
Reduction Method ◽  
Chemical Reduction ◽  
Core Shell ◽  
Chemical Reduction Method

scholarly journals Fabrication and Characterization of a Highly-Sensitive Surface-Enhanced Raman Scattering Nanosensor for Detecting Glucose in Urine

Nanomaterials ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 629 ◽  
Author(s):  
Yudong Lu ◽  
Ting Zhou ◽  
Ruiyun You ◽  
Yang Wu ◽  
Huiying Shen ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Internal Standard ◽  
Surface Enhanced Raman Scattering ◽  
Quantitative Detection ◽  
Core Shell ◽  
Ag Nps ◽  
Vis Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Herein we utilized coordination interactions to prepare a novel core-shell plasmonic nanosensor for the detection of glucose. Specifically, Au nanoparticles (NPs) were strongly linked with Ag+ ions to form a sacrificial Ag shell by using 4-aminothiophenol (4-PATP) as a mediator, which served as an internal standard to decrease the influence of the surrounding on the detection. The resultant Au-PATP-Ag core-shell systems were characterized by UV-vis spectroscopy, transmission electron microscopy, and surface-enhanced Raman scattering (SERS) techniques. Experiments performed with R6G (rhodamine 6G) and CV (crystal violet) as Raman reporters demonstrated that the Au@Ag nanostructure amplified SERS signals obviously. Subsequently, the Au@Ag NPs were decorated with 4-mercaptophenylboronic acid (4-MPBA) to specifically recognize glucose by esterification, and a detection limit as low as 10−4 M was achieved. Notably, an enhanced linearity for the quantitative detection of glucose (R2 = 0.995) was obtained after the normalization of the spectral peaks using 4-PATP as the internal standard. Finally, the practical applicability of the developed sensing platform was demonstrated by the detection of glucose in urine with acceptable specificity.

scholarly journals Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 415 ◽  
Author(s):  
Xian Zhang ◽  
Zuohuan Wang ◽  
Yun Fang ◽  
Renjie Sun ◽  
Tong Cao ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Goodness Of Fit ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Quantitative Detection ◽  
Antibody Microarray ◽  
Test Kit ◽  
Microarray Immunoassay

We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

Colorimetric assay for the simultaneous detection of Hg2+ and Ag+ based on inhibiting the peroxidase-like activity of core–shell Au@Pt nanoparticles

2017 ◽  
Vol 9 (30) ◽  
pp. 4363-4370 ◽  
Author(s):  
Chi-Fang Peng ◽  
Ying-Ying Zhang ◽  
Li-Ying Wang ◽  
Zheng-Yu Jin ◽  
Guang Shao
Keyword(s):  
Colorimetric Assay ◽  
Simultaneous Detection ◽  
Pt Nanoparticles ◽  
Core Shell ◽  
Highly Sensitive

A highly sensitive and selective colorimetric assay for Hg2+ and Ag+ was developed using core–shell Au@Pt nanoparticles.

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