A lateral flow biosensor based on gold nanoparticles detects four hemorrhagic fever viruses

2020 ◽  
Vol 12 (46) ◽  
pp. 5613-5620
Author(s):  
Jin Zhao ◽  
Shuting Fang ◽  
Yujie Liu ◽  
Lingwen Zeng ◽  
Zhixu He
Keyword(s):  
Gold Nanoparticles ◽  
Human Health ◽  
Hemorrhagic Fever ◽  
Lateral Flow ◽  
Fever Virus ◽  
Viral Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus

The pathogen of viral hemorrhagic fever (VHF), which is harmful to human health, is a hemorrhagic fever virus.

scholarly journals Clinical Characterization of Host Response to Simian Hemorrhagic Fever Virus Infection in Permissive and Refractory Hosts: A Model for Determining Mechanisms of VHF Pathogenesis

2018 ◽  
Author(s):  
Joseph P. Cornish ◽  
Ian N. Moore ◽  
Donna L. Perry ◽  
Abigail Lara ◽  
Mahnaz Minai ◽  
...  
Keyword(s):  
Rhesus Monkeys ◽  
Host Response ◽  
Severe Disease ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Host Responses ◽  
Viral Hemorrhagic Fever ◽  
Simian Hemorrhagic Fever Virus ◽  
Patas Monkeys ◽  
Hemorrhagic Fever Virus

ABSTRACTSimian hemorrhagic fever virus (SHFV) causes a fulminant and typically lethal viral hemorrhagic fever (VHF) in macaques (Cercopithecinae: Macaca spp.) but causes subclinical infections in patas monkeys (Cercopithecinae: Erythrocebus patas). This difference in disease course offers a unique opportunity to compare host-responses to infection by a VHF-causing virus in biologically similar susceptible and refractory animals. Patas and rhesus monkeys were inoculated side-by-side with SHFV. In contrast to the severe disease observed in rhesus monkeys, patas monkeys developed a limited clinical disease characterized by changes in complete blood counts, serum chemistries, and development of lymphadenopathy. Viremia was measurable 2 days after exposure and its duration varied by species. Infectious virus was detected in terminal tissues of both patas and rhesus monkeys. Varying degrees of overlap in changes in serum concentrations of IFN-γ, MCP-1, and IL-6 were observed between patas and rhesus monkeys, suggesting the presence of common and species-specific cytokine responses to infection. Similarly, quantitative immunohistochemistry of terminal livers and whole blood flow cytometry revealed varying degrees of overlap in changes in macrophages, natural killer cells, and T-cells. The unexpected degree of overlap in host-response suggests that relatively small subsets of a host’s response to infection may be responsible for driving pathogenesis that results in a hemorrhagic fever. Furthermore, comparative SHFV infection in patas and rhesus monkeys offers an experimental model to characterize host-response mechanisms associated with viral hemorrhagic fever and evaluate pan-viral hemorrhagic fever countermeasures.IMPORTANCEHost-response mechanisms involved in pathogenesis of VHFs remain poorly understood. An underlying challenge is separating beneficial, inconsequential, and detrimental host-responses during infection. The comparison of host-responses to infection with the same virus in biologically similar animals that have drastically different disease manifestations allows for the identification of pathogenic mechanisms. SHFV, a surrogate virus for human VHF-causing viruses likely causes subclinical infection in African monkeys such as patas monkeys but can cause severe disease in Asian macaque monkeys. Data from the accompanying article by Buechler et al. support that infection of macaques and baboons with non-SHFV simarteviruses can establish persistent or long-term subclinical infections. Baboons, macaques, and patas monkeys are relatively closely taxonomically related (Cercopithecidae: Cercopithecinae) and therefore offer a unique opportunity to dissect how host-response differences determine disease outcome in VHFs.

scholarly journals Clinical Characterization of Host Response to Simian Hemorrhagic Fever Virus Infection in Permissive and Refractory Hosts: A Model for Determining Mechanisms of VHF Pathogenesis

Viruses ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 67
Author(s):  
Joseph Cornish ◽  
Ian Moore ◽  
Donna Perry ◽  
Abigail Lara ◽  
Mahnaz Minai ◽  
...  
Keyword(s):  
Rhesus Monkeys ◽  
Host Response ◽  
Severe Disease ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Host Responses ◽  
Viral Hemorrhagic Fever ◽  
Simian Hemorrhagic Fever Virus ◽  
Patas Monkeys ◽  
Hemorrhagic Fever Virus

Simian hemorrhagic fever virus (SHFV) causes a fulminant and typically lethal viral hemorrhagic fever (VHF) in macaques (Cercopithecinae: Macaca spp.) but causes subclinical infections in patas monkeys (Cercopithecinae: Erythrocebus patas). This difference in disease course offers a unique opportunity to compare host responses to infection by a VHF-causing virus in biologically similar susceptible and refractory animals. Patas and rhesus monkeys were inoculated side-by-side with SHFV. Unlike the severe disease observed in rhesus monkeys, patas monkeys developed a limited clinical disease characterized by changes in complete blood counts, serum chemistries, and development of lymphadenopathy. Viral RNA was measurable in circulating blood 2 days after exposure, and its duration varied by species. Infectious virus was detected in terminal tissues of both patas and rhesus monkeys. Varying degrees of overlap in changes in serum concentrations of interferon (IFN)-γ, monocyte chemoattractant protein (MCP)-1, and interleukin (IL)-6 were observed between patas and rhesus monkeys, suggesting the presence of common and species-specific cytokine responses to infection. Similarly, quantitative immunohistochemistry of livers from terminal monkeys and whole blood flow cytometry revealed varying degrees of overlap in changes in macrophages, natural killer cells, and T-cells. The unexpected degree of overlap in host response suggests that relatively small subsets of a host’s response to infection may be responsible for driving hemorrhagic fever pathogenesis. Furthermore, comparative SHFV infection in patas and rhesus monkeys offers an experimental model to characterize host–response mechanisms associated with viral hemorrhagic fever and evaluate pan-viral hemorrhagic fever countermeasures.

scholarly journals Alkhurma Viral Hemorrhagic Fever Virus: Proposed Guidelines for Detection, Prevention, and Control in Saudi Arabia

2012 ◽  
Vol 6 (7) ◽  
pp. e1604 ◽  
Author(s):  
Ziad A. Memish ◽  
Shamsudeen F. Fagbo ◽  
Abdullah M. Assiri ◽  
Pierre Rollin ◽  
Ali M. Zaki ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Prevention And Control ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Viral Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
And Control
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