Gas chromatography-tandem mass spectrometry-based detection of half nitrogen mustards in plasma as a new biomarker of nitrogen mustard exposure

2020 ◽  
Vol 12 (36) ◽  
pp. 4447-4456
Author(s):  
Buddhadeb Chandra ◽  
Kanchan Sinha Roy ◽  
Mahabul Shaik ◽  
Chandrakant Waghmare ◽  
RamaRao Golime ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nitrogen Mustard ◽  
Chemical Warfare Agent ◽  
Chemical Warfare ◽  
Tandem Mass ◽  
Reactive Metabolite ◽  
Nitrogen Mustards ◽  
Blood Samples ◽  
Chromatography Tandem Mass Spectrometry ◽  
Detection And Identification

The development, optimization and validation of an analytical method for the detection and identification of reactive metabolite of nitrogen mustards (a Chemical Warfare Agent), i.e. half nitrogen mustards in blood samples by GC-MS/MS (PCI).

scholarly journals Sex differences in serum levels of 5α-androstane-3β, 17β-diol, and androstenediol in the young adults: A liquid chromatography–tandem mass spectrometry study

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261440
Author(s):  
Haruka Tanabe ◽  
Hitoshi Mutai ◽  
Daimei Sasayama ◽  
Hidehiko Sasamoto ◽  
Yoshimichi Miyashiro ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sex Differences ◽  
Menstrual Cycle ◽  
Rating Scale ◽  
Serum Levels ◽  
Tandem Mass ◽  
Blood Samples ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Hamilton Rating Scale

Animal experiments have consistently shown that estrogen receptor β (ERβ)-selective ligands have antidepressant and anxiolytic effects. In humans, endogenous ligands for ERβ include 5α-androstane-3β, 17β-diol (3βAdiol) and androstenediol (Δ5-diol). We determined, for the first time, the exact serum levels of 3βAdiol and Δ5-diol in young healthy volunteers using liquid chromatography–tandem mass spectrometry (LC–MS/MS). We investigated the effect of the menstrual cycle on the levels of these steroids in women; then, we performed a gender comparison. Blood samples were collected from 48 subjects: 23 women (mean age = 28.4±7.8 years) and 25 men (mean age = 31.4±7.8 years). We collected the blood samples of women at three time-points in the menstrual cycle: the early follicular phase, ovulatory or mid-cycle phase, and mid-luteal phase. A total of 92 blood samples were analyzed using LC–MS/MS. The levels of two well-studied steroids, namely dehydroepiandrosterone (DHEA) and 17β-estradiol (E2), were simultaneously measured. Depression rating scale (Hamilton Rating Scale for Depression, Beck Depression Inventory-II and Quick Inventory of Depressive Symptomatology) scores were also recorded at the time of blood sampling. Significant differences in the levels of 3βAdiol and E2 and in the depression rating scale scores were observed over the duration of the menstrual cycle of the women. The levels of 3βAdiol and Δ5-diol were significantly lower in women than in men. E2 levels were higher in women than in men, and DHEA levels did not differ significantly between men and women. Further, women had higher scores than men on the Hamilton Rating Scale for Depression. Sex differences in depressive symptoms can be explained by 3βAdiol and Δ5-diol levels, and the effect of the menstrual cycle on mood can be explained by 3βAdiol and E2 levels, not by Δ5-diol level.

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