Label-free test kit for d-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles

2020 ◽  
Vol 12 (26) ◽  
pp. 3404-3410
Author(s):  
Yan Zeng ◽  
Peng Qi ◽  
Dun Zhang
Keyword(s):  
Gold Nanoparticles ◽  
Amino Acid ◽  
Amino Acid Analysis ◽  
Label Free ◽  
Test Kit ◽  
Precision And Accuracy ◽  
Induced Aggregation

We proposed a label-free kit test for d-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles, providing an effective and selective quantification of DAAs with excellent precision and accuracy in bacterial samples.

Small Molecule- and Amino Acid-Induced Aggregation of Gold Nanoparticles

Langmuir ◽  
2013 ◽  
Vol 29 (25) ◽  
pp. 7661-7673 ◽  
Author(s):  
Hesham M. Zakaria ◽  
Akash Shah ◽  
Michael Konieczny ◽  
Joan A. Hoffmann ◽  
A. Jasper Nijdam ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Amino Acid ◽  
Small Molecule ◽  
Induced Aggregation

Label-free amino acid detection based on nanocomposites of graphene oxide hybridized with gold nanoparticles

2016 ◽  
Vol 77 ◽  
pp. 963-970 ◽  
Author(s):  
Qian Zhang ◽  
Diming Zhang ◽  
Yanli Lu ◽  
Gang Xu ◽  
Yao Yao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Label Free ◽  
Amino Acid Detection

An ESR study of the peroxidase reaction catalyzed by human methaemoglobin and methaemoglobin-haptoglobin complex

1991 ◽  
Vol 56 (4) ◽  
pp. 923-932
Author(s):  
Jana Stejskalová ◽  
Pavel Stopka ◽  
Zdeněk Pavlíček
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Amino Acid ◽  
Peroxidase Activity ◽  
Amino Acid Analysis ◽  
Superoxide Radical ◽  
Esr Spectra ◽  
The Other ◽  
Delocalized Electron

The ESR spectra of peroxidase systems of methaemoglobin-ascorbic acid-hydrogen peroxide and methaemoglobin-haptoglobin complex-ascorbic acid-hydrogen peroxide have been measured in the acetate buffer of pH 4.5. For the system with methaemoglobin an asymmetrical signal with g ~ 2 has been observed which is interpreted as the perpendicular region of anisotropic spectrum of superoxide radical. On the other hand, for the system with methaemoglobin-haptoglobin complex the observed signal with g ~ 2 is symmetrical and is interpreted as a signal of delocalized electron. After realization of three repeatedly induced peroxidase processes the ESR signal of the perpendicular part of anisotropic spectrum of superoxide radical is distinctly diminished, whereas the signal of delocalized electron remains practically unchanged. An amino acid analysis of methaemoglobin along with results of the ESR measurements make it possible to derive a hypothesis about the role of haptoglobin in increasing of the peroxidase activity of methaemoglobin.

scholarly journals Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rozita Abolhasan ◽  
Balal Khalilzadeh ◽  
Hadi Yousefi ◽  
Sahar Samemaleki ◽  
Forough Chakari-Khiavi ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Flexible Structures ◽  
Modified Electrodes ◽  
Electrochemical Immunosensor ◽  
Lymphocytic Leukemia ◽  
Orphan Receptor ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Limit Of Quantification

AbstractIn the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.

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