Evaluation of different strategies for determination of selenomethionine (SeMet) in selenized yeast by asymmetrical flow field flow fractionation coupled to inductively coupled plasma mass spectrometry (AF4-ICP-MS)

2020 ◽  
Vol 12 (26) ◽  
pp. 3351-3360
Author(s):  
Daniel B. Alcântara ◽  
Ronaldo F. Nascimento ◽  
Gisele S. Lopes ◽  
Patricia Grinberg
Keyword(s):  
Flow Field ◽  
Inductively Coupled Plasma ◽  
Chemical Speciation ◽  
Field Flow Fractionation ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Flow Fractionation ◽  
Selenized Yeast

This manuscript exemplifies the prospective use of asymmetrical flow field flow fractionation (AF4) coupled to ICP-MS as a simple tool for chemical speciation of selemethionine in selenized yeast.

scholarly journals Challenges in Determining the Size Distribution of Nanoparticles in Consumer Products by Asymmetric Flow Field-Flow Fractionation Coupled to Inductively Coupled Plasma-Mass Spectrometry: The Example of Al2O3, TiO2, and SiO2 Nanoparticles in Toothpaste

Separations ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 56 ◽  
Author(s):  
Manuel Correia ◽  
Toni Uusimäki ◽  
Allan Philippe ◽  
Katrin Loeschner
Keyword(s):  
Mass Spectrometry ◽  
Particle Size ◽  
Flow Field ◽  
Inductively Coupled Plasma ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Flow Fractionation

According to the current European regulation on cosmetics, any ingredient present as a nanomaterial should be indicated in the ingredient list. There is a need for analytical methods capable of determining the size of the relevant ingredients and thus assessing if these are nanomaterials or not. An analytical method based on asymmetric flow field-flow fractionation (AF4) and inductively coupled plasma-mass spectrometry (ICP-MS) was developed to determine the size of particles present in a commercial toothpaste. Multi-angle light scattering (MALS) was used for on-line size determination. The number-based particle size distributions (PSDs) of the particles were retrieved upon mathematical conversion of the mass-based PSDs recovered from the AF4-ICP-MS fractograms. AF4-ICP-MS allowed to separate and detect Al2O3 and TiO2 particles in the toothpaste and to retrieve a correct TiO2 number-based PSD. The potential presence of particles in the lower size range of the Al2O3 mass-based PSD had a strong impact on sizing and nanomaterial classification upon conversion. AF4 coupled with ICP-MS and MALS was found to be a powerful approach for characterization of different particles in a multiple-particle system such as toothpaste. Confirmation of particle size by a secondary method such as single particle ICP-MS or hydrodynamic chromatography was crucial.

ICP-MS-based approach to determine nanoparticle recovery after hollow fiber flow field flow fractionation

2021 ◽  
Vol 28 ◽  
Author(s):  
Fabian L. Kriegel ◽  
Benjamin C. Krause ◽  
Yves U. Hachenberger ◽  
Rafael Fister ◽  
Philipp Reichardt ◽  
...  
Keyword(s):  
Flow Field ◽  
Hollow Fiber ◽  
Inductively Coupled Plasma ◽  
Field Flow Fractionation ◽  
Fractionation Process ◽  
Au Nps ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Flow Fractionation

: Compared to classical chemicals, nanoparticles (NPs) exhibit unique properties, which lead to challenges in sample preparation and analysis. Fractionation techniques and in particular hollow fiber flow field flow fractionation (HF5) have recently become popular in the characterization and quantification of nanomaterials, because of their fine fractionation capability in the nanoscale-range. When dealing with NPs a great drawback during fractionation is the loss of particles in the fractionation devices, tubing and connectors. There is a need of studies to systematically explore and assess the quality of the fractionation process. A combination of two complementary mass-based setups was used to determine particle loss in HF5. Inductively coupled plasma mass spectrometry (ICP-MS) enabled the estimation of recovery rates for NPs after HF5 separation. Reciprocally, laser ablation ICP-MS (LA-ICP-MS) permitted the evaluation of particles retained on the hollow fiber. 15 nm Au-NPs in different concentrations were evaluated in this study and showed a recovery level for Au-NPs of 50 – 65 % based on the applied concentrations after a complete HF5 separation run. Detection of sample deposition on the hollow fiber by LA-ICP-MS indicated a sample loss of about 8 %. These findings are important for experiments relying on fractionation of low concentrated nanoparticulate samples.

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