The sensitive detection of single-cell secreted lactic acid for glycolytic inhibitor screening with a microdroplet biosensor

2020 ◽  
Vol 12 (25) ◽  
pp. 3250-3259 ◽  
Author(s):  
Xuyue Chen ◽  
Rui Shen ◽  
Sidi Liu ◽  
Xiang Xiao ◽  
Jun Yan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Single Cell ◽  
Tumor Metabolism ◽  
Malignant Progression ◽  
Sensitive Detection ◽  
Inhibitor Screening ◽  
Glycolytic Inhibitor

Lactic acid (LA) plays an important role in the tumor metabolism and malignant progression of various cancers.

scholarly journals Correction: The sensitive detection of single-cell secreted lactic acid for glycolytic inhibitor screening with a microdroplet biosensor

2020 ◽  
Vol 12 (32) ◽  
pp. 4097-4099
Author(s):  
Xuyue Chen ◽  
Rui Shen ◽  
Sidi Liu ◽  
Xiang Xiao ◽  
Jun Yan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Single Cell ◽  
Sensitive Detection ◽  
Inhibitor Screening ◽  
Glycolytic Inhibitor

Correction for ‘The sensitive detection of single-cell secreted lactic acid for glycolytic inhibitor screening with a microdroplet biosensor’ by Xuyue Chen et al., Anal. Methods, 2020, 12, 3250–3259, DOI: 10.1039/D0AY00633E.

scholarly journals Single-cell RNA Sequencing Reveals Immunosuppressive Myeloid Cell Diversity and Restricted Cytotoxic Effector Cell Trafficking and Activation During Malignant Progression in Glioma

2021 ◽  
Author(s):  
Sakthi Rajendran ◽  
Clayton Peterson ◽  
Alessandro Canella ◽  
Yang Hu ◽  
Amy Gross ◽  
...  
Keyword(s):  
T Cells ◽  
Tumor Microenvironment ◽  
Single Cell ◽  
Immune Cell ◽  
Myeloid Cell ◽  
Malignant Progression ◽  
Low Grade ◽  
Cell Trafficking ◽  
High Grade ◽  
Single Cell Rna Sequencing

Low grade gliomas (LGG) account for about two-thirds of all glioma diagnoses in adolescents and young adults (AYA) and malignant progression of these patients leads to dismal outcomes. Recent studies have shown the importance of the dynamic tumor microenvironment in high-grade gliomas (HGG), yet its role is still poorly understood in low-grade glioma malignant progression. Here, we investigated the heterogeneity of the immune microenvironment using a platelet-derived growth factor (PDGF)-driven RCAS (replication-competent ASLV long terminal repeat with a splice acceptor) glioma model that recapitulates the malignant progression of low to high-grade glioma in humans and also provides a model system to characterize immune cell trafficking and evolution. To illuminate changes in the immune cell landscape during tumor progression, we performed single-cell RNA sequencing on immune cells isolated from animals bearing no tumor (NT), LGG and HGG, with a particular focus on the myeloid cell compartment, which is known to mediate glioma immunosuppression. LGGs demonstrated significantly increased infiltrating T cells, CD4 T cells, CD8 T cells, B cells, and natural killer cells in the tumor microenvironment, whereas HGGs significantly abrogated this infiltration. Our study identified two distinct macrophage clusters in the tumor microenvironment; one cluster appeared to be bone marrow-derived while another was defined by overexpression of Trem2, a marker of tumor associated macrophages. Our data demonstrates that these two distinct macrophage clusters show an immune-activated phenotype (Stat1, Tnf, Cxcl9 and Cxcl10) in LGG which evolves to an immunosuppressive state (Lgals3, Apoc1 and Id2) in HGG that restricts T cell recruitment and activation. We identified CD74 and macrophage migration inhibition factor (MIF) as potential targets for these distinct macrophage populations. Interestingly, these results were mirrored by our analysis of the TCGA dataset, which demonstrated a statistically significant association between CD74 overexpression and decreased overall survival in AYA patients with grade II gliomas. Targeting immunosuppressive myeloid cells and intra-tumoral macrophages within this therapeutic window may ameliorate mechanisms associated with immunosuppression before and during malignant progression.

Sensitive detection of caspase-3 enzymatic activities and inhibitor screening by mass spectrometry with dual maleimide labelling quantitation

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6751-6759
Author(s):  
Fuzhong Ouyang ◽  
Tianrong Yu ◽  
Chao Gu ◽  
Guanghui Wang ◽  
Rui Shi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Enzymatic Activities ◽  
Sensitive Detection ◽  
Inhibitor Screening

There is a great need to develop sensitive and specific methods for quantitative analysis of caspase-3 activities in cell apoptosis.

scholarly journals Author Correction: Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma

Cell Research ◽  
2019 ◽  
Vol 29 (9) ◽  
pp. 777-777
Author(s):  
Junya Peng ◽  
Bao-Fa Sun ◽  
Chuan-Yuan Chen ◽  
Jia-Yi Zhou ◽  
Yu-Sheng Chen ◽  
...  
Keyword(s):  
Single Cell ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Malignant Progression ◽  
Ductal Adenocarcinoma ◽  
Rna Seq

A triple-amplification strategy for sensitive detection of telomerase at the single-cell level

2018 ◽  
Vol 54 (67) ◽  
pp. 9317-9320 ◽  
Author(s):  
Chen-chen Li ◽  
Yan Zhang ◽  
Wen-jing Liu ◽  
Chun-yang Zhang
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Sensitive Detection ◽  
Single Cell Level ◽  
Cell Level ◽  
Amplification Strategy

We develop a triple-amplification strategy for sensitive detection of telomerase from cancer cells at the single-cell level.

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