Bio-assay of the non-amidated progastrin-derived peptide (G17-Gly) using the tailor-made recombinant antibody fragment and phage display method: a biomedical analysis

2020 ◽  
Vol 12 (21) ◽  
pp. 2735-2746
Author(s):  
Deniz Sadighbayan ◽  
Mohammad Reza Tohidkia ◽  
Tayebeh Mehdipour ◽  
Mohammad Hasanzadeh ◽  
Ahmad Yari Khosroushahi
Keyword(s):  
Phage Display ◽  
Signal Amplification ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Electrochemical Determination ◽  
Biomedical Analysis ◽  
Recombinant Antibody Fragment ◽  
Antibody Technology ◽  
Bio Assay

In this research, four novel and sensitive immunosensors for electrochemical determination of G17-Gly were designed based on signal amplification and tailor-made recombinant antibody technology.

High-level accumulation of a recombinant antibody fragment in the periplasm ofEscherichia coli requires a triple-mutant (degP prc spr) host strain

2004 ◽  
Vol 85 (5) ◽  
pp. 463-474 ◽  
Author(s):  
Christina Chen ◽  
Brad Snedecor ◽  
Julie C. Nishihara ◽  
John C. Joly ◽  
Nancy McFarland ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Ofescherichia Coli ◽  
Host Strain ◽  
Triple Mutant ◽  
Recombinant Antibody Fragment ◽  
High Level

Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus 3

2008 ◽  
Vol 153 (6) ◽  
pp. 1075-1084 ◽  
Author(s):  
Martin Orecchia ◽  
Greta Nölke ◽  
Pasquale Saldarelli ◽  
Mariangela Dell’Orco ◽  
Kerstin Uhde-Holzem ◽  
...  
Keyword(s):  
Coat Protein ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Recombinant Antibody Fragment

scholarly journals Isolation and Characterisation of a Recombinant Antibody Fragment That Binds NCAM1-Expressing Intervertebral Disc Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e83678 ◽  
Author(s):  
Claire Cunningham ◽  
Akshay Srivastava ◽  
Estelle Collin ◽  
Sibylle Grad ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Recombinant Antibody Fragment ◽  
Disc Cells

scholarly journals Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli

Medicina ◽  
2021 ◽  
Vol 57 (9) ◽  
pp. 981
Author(s):  
Chang-Hun Yeom ◽  
Hee-Jin Jeong
Keyword(s):  
Escherichia Coli ◽  
Recombinant Antibody ◽  
Antibody Fragment ◽  
Soluble Form ◽  
Efficient Production ◽  
Binding Ability ◽  
Recombinant Scfv ◽  
Recombinant Antibody Fragment ◽  
Recombinant Antibody Fragments ◽  
Metalloproteinase 9

Matrix metalloproteinase 9 (MMP9) is involved in several aspects of the pathology of cancer, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant scFv-type anti-MMP9 antibody in soluble form using Escherichia coli, purified it, and confirmed its antigen-binding ability. The convenient, rapid, inexpressive system used in this study for producing recombinant antibody fragments needs only five days, and thus can be used for the efficient production of scFv against MMP9, which can be used in a range of applications and industrial fields, including diagnosis and treatment of inflammatory and cancer-related diseases.

scholarly journals Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7652
Author(s):  
Wei Sun ◽  
Yan Zhang ◽  
Zhigang Ju
Keyword(s):  
Phage Display ◽  
Recombinant Antibody ◽  
Recombinant Antibodies ◽  
Phage Display Library ◽  
Ease Of Use ◽  
Phage Library ◽  
Phage Display Technology ◽  
Random Peptide ◽  
Display Technology

Mycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins. Based on the special affinity between antibody and antigen, immunoassay has been proved to be a powerful technology for the detection of small analytes. However, the tedious preparation and instability of conventional antibodies restrict its application on easy and fast mycotoxins detection. By virtue of simplicity, ease of use, and lower cost, phage display library provides novel choices for antibodies or hapten conjugates, and lead random peptide or recombinant antibody to becoming the promising and environmental friendly immune-reagents in the next generation of immunoassays. This review briefly describes the latest developments on mycotoxins detection using M13 phage display, mainly focusing on the recent applications of phage display technology employed in mycotoxins detection, including the introduction of phage and phage display, the types of phage displayed peptide/recombinant antibody library, random peptides/recombinant antibodies-based immunoassays, as well as simultaneous determination of multiple mycotoxins.

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