Carbon dots tailored with a fluorophore for sensitive and selective detection of hydrogen sulfide based on a ratiometric fluorescence signal

2020 ◽  
Vol 12 (12) ◽  
pp. 1617-1623
Author(s):  
Guoqiang Liu ◽  
Hongwei Ge ◽  
Ranhao Yin ◽  
Long Yu ◽  
Congming Sun ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Carbon Dots ◽  
Fluorescence Signal ◽  
Selective Detection ◽  
Ratiometric Fluorescence

Hydrogen sulfide reacts to NBD-based derivatives and quenches the fluorescence, while the fluorescence of CDs is enhanced, leading to a distinct ratiometric fluorescence signal.

scholarly journals A ratiometric fluorescence probe for the selective detection of H2S in serum using a pyrene-DPA–Cd2+ complex

RSC Advances ◽  
2021 ◽  
Vol 11 (39) ◽  
pp. 24410-24415
Author(s):  
Jihoon Kim ◽  
Jinyoung Oh ◽  
Min Su Han
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescence Probe ◽  
Selective Detection ◽  
Ratiometric Fluorescence ◽  
Displacement Approach

The pyrene-DPA–Cd2+ complex is demonstrated as a ratiometric fluorescence probe for selective hydrogen sulfide detection in serum based on a metal displacement approach.

scholarly journals Synthesis of Fluorescent Carbon Dots as Selective and Sensitive Probes for Cupric Ions and Cell Imaging

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1785 ◽  
Author(s):  
Shu-Wei Huang ◽  
Yu-Feng Lin ◽  
Yu-Xuan Li ◽  
Cho-Chun Hu ◽  
Tai-Chia Chiu
Keyword(s):  
Carbon Dots ◽  
Cell Imaging ◽  
Water Soluble ◽  
Fluorescence Signal ◽  
Selective Detection ◽  
Cupric Ion ◽  
Sensing System ◽  
Wide Range ◽  
Low Toxicity ◽  
Fluorescent Carbon Dots

A novel sensing system has been designed for the detection of cupric ions. It is based on the quenched fluorescence signal of carbon dots (CDs), which were carbonized from poly(vinylpyrrolidone) (PVP) and L-Cysteine (CYS). Cupric ions interact with the nitrogen and sulfur atoms on surface of the CDs to form an absorbed complex; this results in strong quenching of the fluorescence of the CDs via a fast metal-to-ligand binding affinity. The synthesized water-soluble CDs also exhibited a quantum yield of 7.6%, with favorable photoluminescent properties and good photostability. The fluorescence intensity of the CDs was very stable in high ionic strength (up to 1.0 M NaCl) and over a wide range of pH levels (2.0–12.0). This facile method can therefore develop a sensor that offers reliable, fast, and selective detection of cupric ions with a detection limit down to 0.15 μM and a linear range from 0.5 to 7.0 μM (R2 = 0.980). The CDs were used for cell imaging, observed that they were low toxicity to Tramp C1 cells and exhibited blue and green and red fluorescence under a fluorescence microscope. In summary, the CDs exhibited excellent fluorescence properties, and could be applied to the selective and sensitive detection of cupric ion and multicolor cell imaging.

Ratio imaging of intracellular ion concentration

1992 ◽  
Vol 50 (2) ◽  
pp. 1160-1161
Author(s):  
Stephen R. Bolsover
Keyword(s):  
Individual Cell ◽  
Video Camera ◽  
Field Of View ◽  
Ion Concentration ◽  
Fluorescence Signal ◽  
Ion Imaging ◽  
Ratiometric Fluorescence ◽  
Ratio Imaging ◽  
The Mean ◽  
The Many

The field of intracellular ion concentration measurement expanded greatly in the 1980's due primarily to the development by Roger Tsien of ratiometric fluorescence dyes. These dyes have many applications, and in particular they make possible to image ion concentrations: to produce maps of the ion concentration within living cells. Ion imagers comprise a fluorescence microscope, an imaging light detector such as a video camera, and a computer system to process the fluorescence signal and display the map of ion concentration.Ion imaging can be used for two distinct purposes. In the first, the imager looks at a field of cells, measuring the mean ion concentration in each cell of the many in the field of view. One can then, for instance, challenge the cells with an agonist and examine the response of each individual cell. Ion imagers are not necessary for this sort of experiment: one can instead use a system that measures the mean ion concentration in a just one cell at any one time. However, they are very much more convenient.

scholarly journals Noval Dual-Emission Fluorescence Carbon Dots as a Ratiometric Probe for Cu2+ and ClO− Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1232
Author(s):  
Jiaqing Guo ◽  
Aikun Liu ◽  
Yutian Zeng ◽  
Haojie Cai ◽  
Shuai Ye ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Background Noise ◽  
Good Recovery ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Simple Method ◽  
Real Samples ◽  
Nitrogen Doped ◽  
Probe Concentration ◽  
Ratiometric Probe

The use of carbon dots (CDs) with dual emission based on ratiometric fluorescence has been attracting attention in recent times for more accurate ion detection since they help avoid interference from background noise, probe concentration, and complexity. Herein, novel dual-emission nitrogen-doped CDs (NCDs) were prepared by a simple method for Cu2+ and ClO- detection. The NCDs showed excellent anti-interference ability and selectivity for different emissions. In addition, a good linear relationship was observed between the fluorescence intensity (FI) of the NCD solutions in different emissions with Cu2+ (0–90 μM) and ClO- (0–75 μM). The limits of both Cu2+ detection and ClO- were very low, at 17.7 and 11.6 nM, respectively. The NCDs developed herein also showed a good recovery rate in water for Cu2+ and ClO− detection. Hence, they are expected to have a more extensive application prospect in real samples.

A Bright Nitrogen-doped-Carbon-Dots based Fluorescent Biosensor for Selective Detection of Copper Ions

2021 ◽  
Vol 5 (1) ◽  
pp. 84-92
Author(s):  
Shuting Chen ◽  
Chaoqun Chen ◽  
Jian Wang ◽  
Fang Luo ◽  
Longhua Guo ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Copper Ions ◽  
Selective Detection ◽  
Nitrogen Doped ◽  
Fluorescent Biosensor ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon
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