Entropy-driven amplification strategy-assisted lateral flow assay biosensor for ultrasensitive and convenient detection of nucleic acids

The Analyst ◽  
2021 ◽  
Author(s):  
Shasha Li ◽  
Hongmin Meng ◽  
Hong Zong ◽  
Juan Chen ◽  
Jianjun Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Treatment Measures ◽  
Amplification Strategy ◽  
And Control ◽  
Timely Treatment ◽  
Nucleic Acid Tests

Accurate, sensitive and rapid nucleic acid tests are important to implement timely treatment measures and control the spread of disease. In this work, we developed a novel portable platform for...

scholarly journals Key points of technical review for the registration of SARS-CoV-2 nucleic acid tests in China

Bioanalysis ◽  
2021 ◽  
Author(s):  
Hongran Li ◽  
Tong Jiao ◽  
Peirong Wang ◽  
Juanjuan An ◽  
Gang Deng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Medical Device ◽  
Product Safety ◽  
Technical Review ◽  
Key Points ◽  
Safety Effectiveness ◽  
Unified Command ◽  
And Control ◽  
Nucleic Acid Tests

In response to the outbreak of COVID-19, in accordance with the principles of ‘unified command, early involvement, prompt review and scientific approval’ as well as the requirements of ensuring product safety, effectiveness and controllable quality, the Center for Medical Device Evaluation (CMDE) has issued Key Points of Technical Review for the Registration of SARS-CoV-2 Nucleic Acid Tests ( Key Points) to provide the requirements of tests. Because of the sustainability of the pandemic, more efforts and attempts are needed for SARS-CoV-2 detection and control. This article interprets the Key Points issued by the CMDE and provides certain refinements to wider audiences.

scholarly journals A Perspective on Re-Detectable Positive SARS-CoV-2 Nucleic Acid Results in Recovered COVID-19 Patients

2020 ◽  
pp. 1-5
Author(s):  
Yanfei He ◽  
Yu-Chao Dong
Keyword(s):  
Nucleic Acid ◽  
Animal Experiments ◽  
Patient Discharge ◽  
Clinical Work ◽  
Igg Antibody ◽  
Viral Virulence ◽  
And Control ◽  
Nucleic Acid Tests

ABSTRACT Objectives: There have been reports on re-detectable positive nucleic acid tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in recovered coronavirus disease (COVID-19) patients. In this study, we look at the clinical characteristics, possible causes, pathogenesis, and infectivity of re-detectable positive patients and provide up-to-date information to public health policy planners and clinicians. Methods: By consulting the latest research data and related progress data of re-detectable positive patients, this study addresses the implications that this special group brings to clinical work and disease prevention and control. Results: We discuss in detail the phenomenon of re-detectable positive nucleic acid tests for recovered patients. There are many possible causes of a re-detectable positive, but there is no 1 factor that can fully explain this phenomenon. Conclusions: It can’t be completely ruled out that the re-detectable positive patients are infectious. We should be alert to these re-detectable positive patients becoming chronic virus carriers, and virus serological IgM and IgG antibody tests should be added before patient discharge. It is urgent to find a more powerful evidence-based and virological basis for the integrity of viral ribonucleic acid and the variation of viral virulence with time through cell experiments in vitro and animal experiments in vivo.

scholarly journals One-Tube SARS-CoV-2 Detection Platform Based on RT-RPA and CRISPR/Cas12a

2020 ◽  
Author(s):  
Yangyang Sun ◽  
Lei Yu ◽  
Chengxi Liu ◽  
Wei Chen ◽  
Dechang Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
N Gene ◽  
Clinical Samples ◽  
Nucleic Acid Detection ◽  
Detection Process ◽  
Human Coronaviruses ◽  
Negative Controls

Abstract Background: COVID-19 has spread rapidly around the world, affecting almost every person. When lifting certain mandatory measures for an economic restart, robust surveillance must be established and implemented, with nucleic acid detection for SARS-CoV-2 as an essential component. Methods: We designed RT-RPA (Reverse Transcription and Recombinase Polymerase Isothermal Amplification) primers of RdRp gene and N gene according to the SARS-CoV-2 gene sequence. We optimized the components in the reaction so that the detection process could be carried out in one tube. The specificity was demonstrated through detecting nucleic acid samples from seven human coronaviruses. Clinical samples were used to validate the platform and all results were compared to rRT-PCR. RNA standards diluted by different gradients were used to demonstrate the limit of detection. Furthermore, we have developed a lateral flow assay based on OR-DETECTR for the detection of COVID-19. Results: We have developed a o ne-tube detection platform based on R T- R PA and DNA Endonuclease-Targeted CRISPR Trans Reporter ( DETECTR ) technology, termed OR-DETECTR, to detect SARS-CoV-2. The detection process is completed in one tube, and the time is 50min. The method can specifically detect SARS-CoV-2 from seven human coronaviruses with a low detection limit of 2.5 copies/µl input. Results from six SARS-CoV-2 patient samples, eight samples from patients with fever but no SARS-CoV-2 infection, and one mixed sample from 40 negative controls showed that OR-DETECTR is 100% consistent with rRT-PCR. Furthermore, we have developed a lateral flow assay based on OR-DETECTR for the detection of COVID-19. Conclusions: OR-DETECTR detection platform is rapid, accurate, tube closed, easy-to-operate, and free of large instruments for COVID-19 detection.

scholarly journals A Novel Lateral Flow Assay for Rapid and Sensitive Nucleic Acid Detection of Avibacterium paragallinarum

2021 ◽  
Vol 8 ◽  
Author(s):  
Caiyun Huo ◽  
Donghai Li ◽  
Zhenguo Hu ◽  
Guiping Li ◽  
Yanxin Hu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gel Electrophoresis ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Nucleic Acid Hybridization ◽  
Nucleic Acid Detection ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Infectious Coryza ◽  
Avibacterium Paragallinarum

Avibacterium paragallinarum, the pathogen of infectious coryza, caused a highly contagious respiratory disease that poses a serious threat to chickens. Hence, it is necessary to do diagnostic screening for Av. paragallinarum. Existing technologies have been used for Av. paragallinarum testing, which, however, have some drawbacks such as time consuming and expensive that require well-trained personnel and sophisticated infrastructure, especially when they are limitedly feasible in some places for lack of resources. Nucleic acid hybridization-based lateral flow assay (LFA) is capable of dealing with these drawbacks, which is attributed to the advantages, such low cost, rapid, and simple. However, nucleic acid determination of Av. paragallinarum through LFA method has not been reported so far. In this study, we developed a novel LFA method that employed gold nanoparticle probes to detect amplified Av. paragallinarum dsDNA. Compared with agarose gel electrophoresis, this LFA strip was inexpensive, simple- to- use, and time- saving, which displayed the visual results within 5–8 min. This LFA strip had higher sensitivity that achieved the detection limit of 101 CFU/ml compared with 102 CFU/ml in agarose gel electrophoresis. Besides, great sensitivity was also shown in the LFA strip, and no cross reaction existed for other bacteria. Furthermore, Av. paragallinarum in clinical chickens with infectious coryza were perfectly detected by our established LFA strip. Our study is the first to develop the LFA integrated with amplification and sample preparation techniques for better nucleic acid detection of Av. paragallinarum, which holds great potential for rapid, accurate, and on-site determination methods for early diagnosis of Av. paragallinarum to control further spreading.

Duplex-specific nuclease signal amplification-based fluorescent lateral flow assay for the point-of-care detection of microRNAs

The Analyst ◽  
2021 ◽  
Author(s):  
Hongjuan Wei ◽  
Yongjin Peng ◽  
Zikun Bai ◽  
Zhen Rong ◽  
Shengqi Wang
Keyword(s):  
Signal Amplification ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Amplification Strategy ◽  
Point Of Care Detection

We demonstrate a fluorescent lateral flow assay combined with duplex specific nuclease signal amplification strategy for high-sensitive point-of-care detection of cancer-related miRNA.

Recent advance in exonuclease Ⅲ-assisted target signal amplification strategy for nucleic acid detection

2021 ◽  
Author(s):  
hongyu liu ◽  
Yuhao You ◽  
Youzhuo Zhu ◽  
Heng Zheng
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Molecular Diagnostics ◽  
Mutation Analysis ◽  
Signal Amplification ◽  
Nucleic Acid Detection ◽  
Target Signal ◽  
Forensic Investigations ◽  
Exo Iii ◽  
Amplification Strategy

Detection of nucleic acids have become significantly important in molecular diagnostics, genetics therapy, mutation analysis, forensic investigations and biomedical development, and so on. In recent years, exonuclease Ⅲ (Exo III)...

scholarly journals Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids

Biosensors ◽  
2012 ◽  
Vol 2 (1) ◽  
pp. 32-42 ◽  
Author(s):  
Yuhong Wang ◽  
Catherine Fill ◽  
Sam R. Nugen
Keyword(s):  
Nucleic Acids ◽  
Lateral Flow ◽  
Lateral Flow Assay

scholarly journals Multifunctional Immunoadjuvants for Use in Minimalist Nucleic Acid Vaccines

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 644
Author(s):  
Saed Abbasi ◽  
Satoshi Uchida
Keyword(s):  
Nucleic Acid ◽  
Immune Responses ◽  
Nucleic Acids ◽  
Innate Immune ◽  
Great Promise ◽  
Nucleic Acid Delivery ◽  
Subunit Vaccines ◽  
Adaptive Immune ◽  
Nucleic Acid Vaccines ◽  
And Control

Subunit vaccines based on antigen-encoding nucleic acids have shown great promise for antigen-specific immunization against cancer and infectious diseases. Vaccines require immunostimulatory adjuvants to activate the innate immune system and trigger specific adaptive immune responses. However, the incorporation of immunoadjuvants into nonviral nucleic acid delivery systems often results in fairly complex structures that are difficult to mass-produce and characterize. In recent years, minimalist approaches have emerged to reduce the number of components used in vaccines. In these approaches, delivery materials, such as lipids and polymers, and/or pDNA/mRNA are designed to simultaneously possess several functionalities of immunostimulatory adjuvants. Such multifunctional immunoadjuvants encode antigens, encapsulate nucleic acids, and control their pharmacokinetic or cellular fate. Herein, we review a diverse class of multifunctional immunoadjuvants in nucleic acid subunit vaccines and provide a detailed description of their mechanisms of adjuvanticity and induction of specific immune responses.

scholarly journals Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ruihua Tang ◽  
Hui Yang ◽  
Yan Gong ◽  
Zhi Liu ◽  
XiuJun Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Nucleic Acid Detection ◽  
Analytical Sensitivity
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