Gas-propelled biosensors for quantitative analysis

The Analyst ◽  
2021 ◽  
Author(s):  
Xinli Liu ◽  
yanping wang ◽  
Yanfeng Gao ◽  
Yujun Song
Keyword(s):  
Quantitative Analysis ◽  
Signal Amplification ◽  
Target Recognition ◽  
Quantitative Detection ◽  
Gas Conversion

Gas-propelled biosensors display a simple gas-based signal amplification with quantitative detection features based on the target recognition event in combination with gas propulsion. Due to the liquid–gas conversion, the gas...

Quantitative analysis of direct oral anticoagulant rivaroxaban by terahertz spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3909-3915 ◽  
Author(s):  
Xu Wu ◽  
Liping Wang ◽  
Yan Peng ◽  
Fang Wu ◽  
Jiumei Cao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Oral Anticoagulant ◽  
Terahertz Spectroscopy ◽  
New Method ◽  
Quantitative Detection ◽  
Direct Oral Anticoagulant ◽  
Qualitative And Quantitative

A new method for the qualitative and quantitative detection of direct oral anticoagulant rivaroxaban by terahertz spectroscopy.

Quantitative detection of harmful elements in alloy steel by LIBS technique and sequential backward selection-random forest (SBS-RF)

2017 ◽  
Vol 32 (11) ◽  
pp. 2194-2199 ◽  
Author(s):  
Fangqi Ruan ◽  
Juan Qi ◽  
Chunhua Yan ◽  
Hongsheng Tang ◽  
Tianlong Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Random Forest ◽  
Alloy Steel ◽  
Quantitative Detection

LIBS technique coupled with SBS-RF was proposed to perform quantitative analysis of S and P in alloy steel samples.

scholarly journals Europium Fluorescent Nanoparticles-Based Multiplex Lateral Flow Immunoassay for Simultaneous Detection of Three Antibiotic Families Residue

2021 ◽  
Vol 9 ◽  
Author(s):  
Yaping Wang ◽  
Biao Ma ◽  
Miaomiao Liu ◽  
Erjing Chen ◽  
Ying Xu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Quantitative Analysis ◽  
Uv Light ◽  
Simultaneous Detection ◽  
Rapid Screening ◽  
Quantitative Detection ◽  
Fluorescent Nanoparticles ◽  
Antibiotic Residues ◽  
Detection Range ◽  
Relative Standard

A fluorescent immunoassay based on europium nanoparticles (EuNPs-FIA) was developed for the simultaneous detection of antibiotic residues, solving the problems of single target detection and low sensitivity of traditional immunoassay methods. In the EuNPs-FIA, EuNPs were used as indictive probes by binding to anti-tetracyclines monoclonal antibodies (anti-TCs mAb), anti-sulphonamides monoclonal antibodies (anti-SAs mAb) and anti-fluoroquinolones monoclonal antibodies (anti-FQs mAb), respectively. Different artificial antigens were assigned to different regions of the nitrocellulose membrane as capture reagents. The EuNPs-FIA allowed for the simultaneous detection of three classes of antibiotics (tetracyclines, fluoroquinolones and sulphonamides) within 15 min. It enabled both the qualitative determination with the naked eye under UV light and the quantitative detection of target antibiotics by scanning the fluorescence intensity of the detection probes on the corresponding detection lines. For qualitative analysis, the cut-off values for tetracyclines (TCs), fluoroquinolones (FQs) and sulphonamides (SAs) were 3.2 ng/ml, 2.4 ng/ml and 4.0 ng/ml, respectively, which were much lower than the maximum residue limit in food. For quantitative analysis, these ranged from 0.06 to 6.85 ng/ml for TCs, 0.03–5.14 ng/ml for FQs, and 0.04–4.40 ng/ml for SAs. The linear correlation coefficients were higher than 0.97. The mean spiked recoveries ranged from 92.1 to 106.2% with relative standard deviations less than 8.75%. Among them, the three monoclonal antibodies could recognize four types of TCs, seven types of FQs and 13 types of SAs, respectively, and the detection range could cover 24 antibiotic residues with different structural formulations. The results of the detection of antibiotic residues in real samples using this method were highly correlated with those of high performance liquid chromatography (R2 > 0.98). The accuracy and precision of the EuNPs-FIA also met the requirements for quantitative analysis. These results suggested that this multiplex immunoassay method was a promising method for rapid screening of three families of antibiotic residues.

scholarly journals Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Chen Chen ◽  
Jia Chen ◽  
Yan Zhang ◽  
Yongbo Li ◽  
Zan Wang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Analysis ◽  
Copy Number ◽  
Meat Products ◽  
Quantitative Detection ◽  
Chain Reaction ◽  
Dna Copy Number ◽  
Meat Weight ◽  
Polymerase Chain ◽  
Digital Polymerase Chain Reaction

A single-copy specific primer was designed based on beef and duck samples and through drop digital polymerase chain reaction (ddPCR) for the quantitative analysis. Results revealed that the primers had no specific amplification with sheep, chicken, pork, or other species. Both the relationships between meat weight and DNA weight and between DNA weight and DNA copy number (C) were nearly linear within the dynamic range. To calculate the original meat weight from the DNA copy number, the DNA weight was used as the intermediate value to establish the following formulae: Mbeef = 0.058C − 1.86; Mduck = 0.0268C − 7.78. To achieve a good quantitative analysis, all species used in the experiment were made of lean meat. The accuracy of the method was verified by artificial adulteration of different proportions. Testing of the commercial samples indicated that adulteration is present in the market. The established digital PCR method provided an effective tool for monitoring the adulterated meat products and reducing the adulteration in the market.

Portable and sensitive quantitative detection of DNA using personal glucose meters and exonuclease III-assisted signal amplification

The Analyst ◽  
2014 ◽  
Vol 139 (19) ◽  
pp. 4982-4986 ◽  
Author(s):  
Xu Xue-tao ◽  
Liang Kai-yi ◽  
Zeng Jia-ying
Keyword(s):  
Signal Amplification ◽  
Detection Method ◽  
Dna Detection ◽  
Quantitative Detection ◽  
Exonuclease Iii

A portable and sensitive quantitative DNA detection method using personal glucose meters and Exonuclease III-assisted signal amplification.

Electrochemical DNA sensor for specific detection of picomolar Hg(ii) based on exonuclease III-assisted recycling signal amplification

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 2029-2036 ◽  
Author(s):  
Xiaorong Gan ◽  
Huimin Zhao ◽  
Shuo Chen ◽  
Xie Quan
Keyword(s):  
Coordination Chemistry ◽  
Signal Amplification ◽  
Quantitative Detection ◽  
Dna Sensor ◽  
Specific Detection ◽  
Exonuclease Iii ◽  
Electrochemical Dna Sensor

An ultrasensitive methodology was successfully developed for the quantitative detection of picomolar Hg2+ based on the combination of thymine–Hg2+–thymine (T–Hg2+–T) coordination chemistry and exonuclease III-aided recycling signal amplification.

Indirect signal amplification strategy with universal probes-based lateral flow immunoassay for rapid quantitative detection of Fumonisin B1.

2022 ◽  
Author(s):  
Chuanyun Zha ◽  
Xingshuang An ◽  
Jiali Zhang ◽  
Lin Wei ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Fumonisin B1 ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Universal Detection ◽  
Amplification Strategy

Fumonisin B1 (FB1) is a serious threat to the health of humans and animals. Herein, a lateral flow immunoassay based on the universal detection probes (goat anti-mouse IgG@Eu ) that...

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