A sandwich-type surface-enhanced Raman scattering sensor using dual aptamers and gold nanoparticles for the detection of tumor extracellular vesicles

The Analyst ◽  
2020 ◽  
Vol 145 (19) ◽  
pp. 6232-6236
Author(s):  
Min Hou ◽  
Dinggeng He ◽  
Hongchang Bu ◽  
Huizhen Wang ◽  
Jin Huang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Raman Scattering ◽  
Extracellular Vesicles ◽  
Surface Enhanced Raman Scattering ◽  
Raman Signal ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sandwich Type ◽  
Enhanced Raman Scattering

A sandwich-type surface-enhanced Raman scattering (SERS) sensor using dual aptamers and gold-enhanced Raman signal probes has been successfully constructed for the detection of tumor-derived extracellular vesicles.

Multi-branched gold nanoparticles for Surface Enhanced Raman scattering characterization

2014 ◽  
Vol 1625 ◽  
Author(s):  
Jencilin Johnston ◽  
Erik N. Taylor ◽  
Richard J. Gilbert ◽  
Thomas J. Webster
Keyword(s):  
Gold Nanoparticles ◽  
Raman Scattering ◽  
Noble Metals ◽  
Fluorescent Dyes ◽  
Synthesis Method ◽  
Surface Enhanced Raman Scattering ◽  
Raman Signal ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

ABSTRACTSurface enhanced Raman scattering (SERS) is a sensitive and reproducible vibrational spectroscopic technique used to detect and characterize molecules near the surface of noble metals like Au, Ag, Pt, Cu, etc. SERS enhances Raman signals through light-induced plasmonic vibrations occurring on irregular metal surfaces and localized electromagnetic augmentation. To better define nano-scale regions of the Raman signal enhancement, we generated gold nanoparticles with a unique multi-branched configuration along with surface-adsorbed fluorescent reporter molecules. The reporter molecules included a set of near-infra red active fluorescent dyes IR820 (green cyanine, photo electronic dye), DTTC (3, 3'-diethylthiatricarbocyanine iodide) and DTDC (3, 3'- diethylthiadicarbocyanine iodide). We employed a one-pot synthesis method in order to generate a stellate configuration in gold nanoparticles through the reduction of HAuCl4 with Good’s buffer, HEPES, at pH 7.4 and room temperature. A cell viability assay was performed with normal esophageal cells exposed to the multi-branched gold nanoparticles and SERS molecules to assess their toxicity. Our results demonstrate the capacity of multibranched gold nanoparticles linked to Raman reporter molecules to generate distinct signature spectra and, with the exception of the gold nanoparticles functionalized with DTTC, remain non-toxic to normal esophageal cells.

scholarly journals Surface enhanced Raman scattering of extracellular vesicles for cancer diagnostics despite isolation dependent lipoprotein contamination

Nanoscale ◽  
2021 ◽  
Author(s):  
Hanna Koster ◽  
Tatu Rojalin ◽  
Alyssa Powell ◽  
Dina Pham ◽  
Rachel Mizenko ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Extracellular Vesicles ◽  
Surface Enhanced Raman Scattering ◽  
Cancer Diagnostics ◽  
Diagnostic Utility ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Particle Counts

Given the emerging diagnostic utility of extracellular vesicles (EVs), it is important to account for non-EV contaminants. Lipoprotein present in EV-enriched isolates may inflate particle counts and decrease sensitivity to...

scholarly journals A Biomedical Surface Enhanced Raman Scattering Substrate: Functionalized Three-Dimensional Porous Membrane Decorated with Silver Nanoparticles

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Li Yuan ◽  
Jinghuai Fang ◽  
Yonglong Jin ◽  
Chaonan Wang ◽  
Tian Xu
Keyword(s):  
Silver Nanoparticles ◽  
Raman Scattering ◽  
Three Dimensional ◽  
Surface Enhanced Raman Scattering ◽  
Porous Membrane ◽  
Raman Signal ◽  
Functional Surface ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

We fabricated a simple, cheap, and functional surface enhanced Raman scattering substrate for biomedical application. Hot spots between two close silver nanoparticles distributed in the skeleton of a three-dimensional porous membrane, especially in the pores, were formed. The dual poles of micropores in the membrane were discussed. The pores could protect the silver nanoparticles in the pores from being oxidized, which makes the membrane effective for a longer period of time. In addition,Staphylococcus aureuscells could be trapped by the micropores and then the Raman signal became stronger, indicating that the functional surface enhanced Raman scattering substrate is reliable.

SERS-active metal–organic frameworks with embedded gold nanoparticles

The Analyst ◽  
2017 ◽  
Vol 142 (14) ◽  
pp. 2640-2647 ◽  
Author(s):  
Xiaolin Cao ◽  
Sihui Hong ◽  
Zejun Jiang ◽  
Yongxin She ◽  
Shanshan Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Raman Scattering ◽  
Metal Organic Frameworks ◽  
Surface Enhanced Raman Scattering ◽  
Active Metal ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Metal Organic ◽  
Enhanced Raman Scattering

Surface-enhanced Raman scattering (SERS) has been widely used in the detection of targets and strongly depends on the interaction and the distance between the targets and nanoparticles.

Finite-Size Effects and Surface-Enhanced Raman Scattering in Noble-Metal Nanoparticles

2004 ◽  
Vol 818 ◽  
Author(s):  
Vitaliy N. Pustovit ◽  
Tigran V. Shahbazyan
Keyword(s):  
Raman Scattering ◽  
Metal Nanoparticles ◽  
Noble Metal ◽  
Finite Size ◽  
Surface Enhanced Raman Scattering ◽  
Noble Metal Nanoparticles ◽  
Raman Signal ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

AbstractWe study the role of a strong electron confinement on the surface-enhanced Raman scattering from molecules adsorbed on small noble-metal nanoparticles. We describe a novel enhancement mechanism which originates from the different effect that confining potential has on s-band and d-band electrons. We demonstrate that the interplay between finite-size and screening efects in the nanoparticle surface layer leads to an enhancement of the surface plasmon local field acting on a molecule located in a close proximity to the metal surface. Our calculations show that the additional enhancement of the Raman signal is especially strong for small nanometer-sized nanoparticles.

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