Rapid genotypic antibiotic susceptibility test using CRISPR-Cas12a for urinary tract infection

The Analyst ◽  
2020 ◽  
Vol 145 (15) ◽  
pp. 5226-5231
Author(s):  
Juhong Chen ◽  
Fuguo Jiang ◽  
Chao-Wei Huang ◽  
Liwei Lin
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
Good Accuracy ◽  
Susceptibility Test ◽  
New Method ◽  
Antibiotic Susceptibility Test

A new method based on the technique of CRISPR-Cas12a is developed to accomplish a bacterial antibiotic susceptibility test (AST) within one hour with good accuracy.

scholarly journals 1472. Non-Carbapenems for Treating Community-Associated Urinary Tract Infection Caused by Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Children

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S537-S537
Author(s):  
Hyun Joo Jung ◽  
Shin Young Lee
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
University Hospital ◽  
E Coli ◽  
Extended Spectrum ◽  
Antibiotic Susceptibility Test ◽  
Higher Sensitivity

Abstract Background Childhood urinary tract infection (UTI) may cause increased major morbidity and long-term clinical consequences. Extended-spectrum β-lactamase (ESBL) is produced by the members of the Enterobacteriaceae family, which are the primary infectious agents that cause UTI in children. Isolation of ESBL-producing Enterobacteriaceae (ESBL-E) typically occurred in healthcare facilities; however, the incidence of community-associated (CA) UTIs due to ESBL-E has increased worldwide. It has led to an increase in the use of carbapenems. In this study, we determine the characteristics of community-onset UTIs caused by ESBL-E in children to suggest non-carbapenem options for the treatment of childhood UTIs due to ESBL-E in order to preserve carbapenems. Methods A total of 2,157 isolates of ESBL-E were collected from children below 18 years old who were clinically certified UTI or urosepsis between January 2008 and August 2018 at tertiary university hospital in Korea. Their electronic medical records were retrospectively reviewed. Long-term healthcare facility stay within the preceding month and isolates recovered more than 72 hours after hospitalization were the criteria of healthcare-associated (HA) infection. Results The most common isolates were E. coli 1815 (84.2%) followed by K. pneumoniae 342 (15.8%). CA infection was detected in 1,513 of the 2157 ESBL-E (70.1%). The prevalence of CA ESBL-E infection increased significantly from 68 cases in 2008 to 325 cased in 2017. Antibiotic susceptibility test showed highest sensitivity to ertapenem, meropenem, and amikacin (>90%) followed by cefoxitin (82%), and piperacillin–tazobactam (TZP) (80.5%). CA E. coli showed higher sensitivity to amikacin and TZP compared with HA E coli. CA K. pneumoniae showed much higher sensitivity to TZP compared with HA K. pneumoniae. Of total ESBL-E, the antimicrobial resistance rate to aminoglycoside such as amikacin and gentamicin showed full sensitivity during the study period; furthermore, a rate of resistance to TZP has been decreasing over the years. Conclusion Identifying antibiotic susceptibility patterns of ESBL-E is a useful guide for treatment strategy of UTI. This study showed that there are non-carbapenem options for the treatment of CA ESBL UTI in children. Disclosures All authors: No reported disclosures.

scholarly journals Prevalence of Asymptomatic Bacteriuria during Pregnancy at a Tertiary Care Hospital of Province No. 2, Nepal

2019 ◽  
Vol 6 ◽  
pp. 32-38
Author(s):  
Khushbu Yadav ◽  
Satyam Prakash
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Antibiotic Susceptibility ◽  
Tertiary Care ◽  
Asymptomatic Bacteriuria ◽  
Diffusion Method ◽  
Care Hospital ◽  
Antibiotic Susceptibility Test

Objectives: The objective of this study was to determine the prevalence of asymptomatic bacteriuria in pregnant women, identify the causative agent responsible for urinary tract infection (UTI) and its antibiotic susceptibility. Methods: The mid-stream urine sample was streaked on the MacConkey agar (MA) and Blood agar (BA) medium by the semi-quantitative culture technique. Identification of significant isolates was done by standard microbiological techniques. Antibiotic susceptibility test of the isolated organisms was done by modified Kirby Bauer disc diffusion method. Results: The prevalence rate of asymptomatic urinary tract infection (AUTI) among pregnant women was found to be 42%. The highest number of UTI cases found during pregnancy was in between age 21-25 years (52.22%), in second gravida (51.59%), during 3rd trimester of pregnancy (49.68%) and in winter with 52.22%. E. coli was principal organism to cause AUTI (35.48%) during pregnancy. Amikacin, imipenem and nalidixic acid were effective towards Gram negative bacilli whereas vancomycin, tetracycline and amoxyclav were effective towards Gram positive cocci. Conclusion: All pregnant women visited for antenatal checkups should be advised for the culture and sensitivity test of their urine specimens which will reduce the maternal and child health complications. Different screening test and awareness programme should be conducted at regular interval of time for prevention of AUTI during pregnancy.

Microbial spectrum and antibiotic susceptibility of uropathogens causing Urinary tract infection in pregnant women

2011 ◽  
Vol 1 (2) ◽  
pp. 77-80
Author(s):  
Pallavi Jayavanth ◽  
Junainah Abd Hamid ◽  
Mathan Mohan ◽  
Mohamed Rafi ◽  
Rajasegar Anamalley ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Antibiotic Susceptibility ◽  
Antibiotic Resistant ◽  
Isolation And Characterization ◽  
Antimicrobial Surveillance ◽  
Antibiotic Susceptibility Test ◽  
Urine Specimens

Urinary tract infection (UTI) is an intermittent cause of sickness in the pregnant women. A study was conducted to identify the microbial gamut and antibiotic vulnerability patterns of bacteria isolated from pregnant women with suspected urinary tract infection. In total, 75 urine specimens were screened for bacteriuria over a period of four months. Aseptically collected urine specimens were subjected to standard isolation and characterization procedures. Antibiotic susceptibility test was performed according to CLSI (2007). Five types of bacteria were isolated namely Escherichia coli 32(55%), Staphylococcus aureus 12 (21%), Enterococcus Sp 7 (12%), Klebseilla pneumoniae 4 (7%) and Pseudomonas aeruginosa 3 (5%). All bacterial isolates exhibited efficacy to all the antibiotics namely fosfomycin (50mg), ciprofloxacin (10mg), trimethoprim & sulfamethoxazole (25 mg), ampicillin (10 mg), nitrofurantoin (300 mg) and cefazolin (30mg) except amoxycillin. Ampicillin and ciprofloxacin exhibited illustrious efficacy at low concentration. Regular antimicrobial surveillance and reinforcement of appropriate antibiotic regimen in pregnant women with UTI will restrain the re‐emergence of antibiotic resistant uropathogens.

scholarly journals Urinary Tract Infection among Patients Visiting Ganesh Man Singh Memorial Hospital and Research Center, Lalitpur, Nepal

2019 ◽  
Vol 6 ◽  
pp. 108-112
Author(s):  
Janak Raj Dhungana ◽  
Aruna Budhathoki ◽  
Goma Poudel ◽  
Jyotika Basnet ◽  
Ravi Shah
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Sensitivity ◽  
Memorial Hospital ◽  
Diffusion Method ◽  
Gram Negative ◽  
Microbiological Methods ◽  
Sensitivity Pattern ◽  
Antibiotic Susceptibility Test

Objectives: The objective of this study was to determine the prevalence of urinary tract infection (UTI) and antibiotic sensitivity pattern among the suspected UTI cases visiting at Ganeshman Singh Memorial Hospital Lalitpur, Nepal. Methods: A total of 300 mid-stream urine, catheter and suprapubic aspirate from UTI suspected patients were included and processed for routine microscopy and culture and then identified by standard microbiological methods. Antibiotic susceptibility test was performed by Kirby- Bauer disc diffusion method. Results: Out of 300 samples, 55(84.6%) mid-stream urine and 10(15.4%) catheter sample had significant bacterial growth. E. coli (32,49.2%) was the most common isolate followed by Staphylococcus aureus (10,15.3%), Enterobacter spp. (8,12.3%), Klebsiella spp. (7,10.7%), Pseudomonas aeruginosa (3,4.6%), Proteus spp.  (3,4.6%), Acinetobacter spp. (1,1.5%) and Enterococcus spp. (1,1.5%). Most of the Gram-negative bacterial isolates were sensitive to Ceftriaxone (88.8%) followed by Gentamicin (72.2%), and Nitrofurantoin (64.8%) and resistant to Amoxicilin (68.5%) followed by Nalidixic Acid (53.7%). Gram positive isolates were sensitive to Amikacin (72.7%) followed by Imipenem (63.6%) and Gentamicin (63.6%) whereas resistant to Amoxycilin (72.7%) and Ciprofloxacin (63.63%). Conclusion: The main cause of the UTIs was found as Gram negative bacteria. Prescription of antibiotics based on susceptibility tests would help in reduction of antibiotic resistance.

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