Nano-Affi: a solution-phase, label-free, colorimetric aptamer affinity assay based on binding-inhibited aggregation of gold nanoparticles

The Analyst ◽  
2020 ◽  
Vol 145 (12) ◽  
pp. 4276-4282 ◽  
Author(s):  
Yuan Wan ◽  
Jiaxing Zhao ◽  
Junlin He ◽  
Xinhui Lou
Keyword(s):  
Gold Nanoparticles ◽  
Solution Phase ◽  
Surface Immobilization ◽  
Label Free ◽  
Native State ◽  
Affinity Assay ◽  
The Ideal

The ideal way to assess aptamer affinity is when both aptamer and target are in a native state, without the unpredictable interference associated with labelling and surface immobilization.

scholarly journals Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rozita Abolhasan ◽  
Balal Khalilzadeh ◽  
Hadi Yousefi ◽  
Sahar Samemaleki ◽  
Forough Chakari-Khiavi ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Flexible Structures ◽  
Modified Electrodes ◽  
Electrochemical Immunosensor ◽  
Lymphocytic Leukemia ◽  
Orphan Receptor ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Limit Of Quantification

AbstractIn the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.

scholarly journals Disulfide-induced self-assembled targets: A novel strategy for the label free colorimetric detection of DNAs/RNAs via unmodified gold nanoparticles

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ehsan Shokri ◽  
Morteza Hosseini ◽  
Mehdi D. Davari ◽  
Mohammad R. Ganjali ◽  
Maikel P. Peppelenbosch ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Label Free ◽  
Self Assembled ◽  
Novel Strategy

Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5

2012 ◽  
Vol 48 (48) ◽  
pp. 6037 ◽  
Author(s):  
Hajra Basit ◽  
K. Shivaji Sharma ◽  
Angéline Van der Heyden ◽  
Chantal Gondran ◽  
Cécile Breyton ◽  
...  
Keyword(s):  
Surface Immobilization ◽  
Label Free ◽  
Label Free Detection

Label-free electrochemiluminescence assay for aqueous Hg2+ through oligonucleotide mediated assembly of gold nanoparticles

2017 ◽  
Vol 98 ◽  
pp. 134-139 ◽  
Author(s):  
Dong-Mei Wang ◽  
Qi-Qi Gai ◽  
Rong-Fu Huang ◽  
Xingwang Zheng
Keyword(s):  
Gold Nanoparticles ◽  
Label Free

Layer-by-layer self-assembly of 2D graphene nanosheets, 3D copper oxide nanoflowers and 0D gold nanoparticles for ultrasensitive electrochemical detection of alpha fetoprotein

RSC Advances ◽  
2015 ◽  
Vol 5 (70) ◽  
pp. 56583-56589 ◽  
Author(s):  
Yulan Wang ◽  
Dan Wu ◽  
Yong Zhang ◽  
Xiang Ren ◽  
Yaoguang Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Copper Oxide ◽  
Electrochemical Detection ◽  
Self Assembly ◽  
Graphene Nanosheets ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Quantitative Detection ◽  
Layer By Layer ◽  
Label Free

In this work, a novel and ultrasensitive label-free electrochemical immunosensor was developed for the quantitative detection of alpha fetoprotein (AFP).

A Fluorescence Sensor for Lead (II) Ions Determination Based on Label-Free Gold Nanoparticles (GNPs)-DNAzyme Using Time-Gated Mode in Aqueous Solution

2016 ◽  
Vol 27 (2) ◽  
pp. 643-649 ◽  
Author(s):  
Xiao-Yu Wang ◽  
Cheng-Gang Niu ◽  
Li-Juan Guo ◽  
Liu-Yin Hu ◽  
Sheng-Quan Wu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Gold Nanoparticles ◽  
Fluorescence Sensor ◽  
Label Free ◽  
Free Gold
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