Metabolic fingerprinting of cell types in mouse skeletal muscle by combining TOF-SIMS with immunofluorescence staining

The Analyst ◽  
2020 ◽  
Vol 145 (21) ◽  
pp. 6901-6909 ◽  
Author(s):  
Zhe Song ◽  
Zhaoying Wang ◽  
Hansen Zhao ◽  
Lesi Cai ◽  
Zhanping Li ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Cell Types ◽  
Skeletal Muscle Tissue ◽  
Immunofluorescence Staining ◽  
Immunofluorescent Staining ◽  
Fiber Types ◽  
Mouse Skeletal Muscle ◽  
Tof Sims ◽  
Different Cell Types

Skeletal muscle tissue is composed of various fiber types which differ in metabolic capacities. TOF-SIMS was combined with immunofluorescent staining to investigate metabolic fingerprints among different cell types in mouse skeletal muscle tissue.

scholarly journals Current Strategies for the Regeneration of Skeletal Muscle Tissue

2021 ◽  
Vol 22 (11) ◽  
pp. 5929
Author(s):  
Emine Alarcin ◽  
Ayca Bal-Öztürk ◽  
Hüseyin Avci ◽  
Hamed Ghorbanpoor ◽  
Fatma Dogan Guzel ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Muscle Regeneration ◽  
Striated Muscle ◽  
Cell Types ◽  
Skeletal Muscle Tissue ◽  
Skeletal Muscle Regeneration ◽  
Muscle Repair ◽  
Clinical Implementation ◽  
Different Cell Types

Traumatic injuries, tumor resections, and degenerative diseases can damage skeletal muscle and lead to functional impairment and severe disability. Skeletal muscle regeneration is a complex process that depends on various cell types, signaling molecules, architectural cues, and physicochemical properties to be successful. To promote muscle repair and regeneration, various strategies for skeletal muscle tissue engineering have been developed in the last decades. However, there is still a high demand for the development of new methods and materials that promote skeletal muscle repair and functional regeneration to bring approaches closer to therapies in the clinic that structurally and functionally repair muscle. The combination of stem cells, biomaterials, and biomolecules is used to induce skeletal muscle regeneration. In this review, we provide an overview of different cell types used to treat skeletal muscle injury, highlight current strategies in biomaterial-based approaches, the importance of topography for the successful creation of functional striated muscle fibers, and discuss novel methods for muscle regeneration and challenges for their future clinical implementation.

scholarly journals High-dimensional single-cell cartography reveals novel skeletal muscle resident cell populations

2018 ◽  
Author(s):  
Lorenzo Giordani ◽  
Gary J. He ◽  
Elisa Negroni ◽  
Hiroshi Sakai ◽  
Justin Y. C. Law ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Single Cell ◽  
Muscle Tissue ◽  
Cell Types ◽  
Skeletal Muscle Tissue ◽  
High Dimensional ◽  
Cell Populations ◽  
Adult Tissue ◽  
Adult Skeletal Muscle ◽  
Different Cell Types

AbstractAdult tissue repair and regeneration require the activation of resident stem/progenitor cells that can self-renew and generate differentiated progeny. The regenerative capacity of skeletal muscle relies on muscle satellite cells (MuSCs) and their interplay with different cell types within the niche. Yet, our understanding of the cells that compose the skeletal muscle tissue is limited and molecular definitions of the principal cell types are lacking. Using a combined approach of single-cell RNA-sequencing and mass cytometry, we precisely mapped the different cell types in adult skeletal muscle tissue and highlighted previously overlooked populations. We identified known functional populations, characterized their gene signatures, and determined key markers. Among the ten main cell populations present in skeletal muscle, we found an unexpected complexity in the interstitial compartment and identified two new cell populations. One express the transcription factor Scleraxis and generate tenocyte-like cells. The second express smooth muscle and mesenchymal cell markers (SMMCs). While distinct from MuSCs, SMMCs are endowed with myogenic potential and promote MuSC engraftment following transplantation. Our high-dimensional single-cell atlas uncovers principles of an adult tissue composition and can be exploited to reveal unknown cellular sub-fractions that contribute to tissue regeneration.

scholarly journals Exclusive vital labeling of myonuclei for studying myonuclear arrangement in mouse skeletal muscle tissue

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Robert Louis Hastings ◽  
Ryan T. Massopust ◽  
Seth G. Haddix ◽  
Young il Lee ◽  
Wesley J. Thompson
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue ◽  
Mouse Skeletal Muscle

scholarly journals Differential Gene Expression in Cell Types of the Human Skeletal Muscle: A Bioinformatics-Based Meta-Review

2021 ◽  
Vol 30 (4) ◽  
pp. 444-452
Author(s):  
Kyung-Wan Baek ◽  
So-Jeong Kim ◽  
Ji-Seok Kim ◽  
Sun-Ok Kwon
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Cultured Cells ◽  
Cell Types ◽  
Gene Expression Omnibus ◽  
Skeletal Muscle Tissue ◽  
Exercise Science ◽  
Ncbi Gene Expression Omnibus ◽  
Genes Encoding

PURPOSE: This study evaluates the differences in the expression of genes frequently analyzed in the field of exercise science between the skeletal muscle tissue and various cell types that comprise the skeletal muscle tissue.METHODS: We summarized the genes and proteins expressed in the skeletal muscle that were published in “Exercise Science” journal from 2015 to present. Thereafter, we selected 15 genes and proteins that were the most analyzed genes and proteins in the skeletal muscle. These genes and proteins were horizontally compared for expression differences in skeletal muscle components and cultured cells based on NCBI Gene Expression Omnibus DataSets.RESULTS: The most analyzed genes (encoding analyzed proteins) in skeletal muscle tissues in “Exercise Science” were PPARGC1A, PPARD, MTOR, MAP1LC3A, MAP1LC3B, PRKAA1, AKT1, SLC2A4, MAPK1, COX4I1, MAPK14, MEF2A, MAPK8, RPS6KB1, and SOD1. Among them, PPARGC1A, AKT1, SLC2A4, MAPK1, and COX4I1 were specifically expressed in the skeletal muscle. However, expression of other genes was found to be significantly affected in other cell types of the skeletal muscle tissue.CONCLUSIONS: Genes such as PPARGC1A, which are specifically expressed in the skeletal muscle, may be analyzed without pretreating (such as perfusion) the skeletal muscle tissue. However, expression of other genes may depend on the skeletal muscle cell type. Thus, in such instances, pretreatment, such as perfusion and isolation, should be considered.

scholarly journals Biochemical Isolation of Myonuclei from Mouse Skeletal Muscle Tissue

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (24) ◽  
Author(s):  
Alicia Cutler ◽  
Anita Corbett ◽  
Grace Pavlath
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue ◽  
Mouse Skeletal Muscle

scholarly journals Proliferation of Multiple Cell Types in the Skeletal Muscle Tissue Elicited by Acute p21 Suppression

2015 ◽  
Vol 23 (5) ◽  
pp. 885-895 ◽  
Author(s):  
Maria Grazia Biferi ◽  
Carmine Nicoletti ◽  
Germana Falcone ◽  
Eleonora M R Puggioni ◽  
Nunzia Passaro ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Cell Types ◽  
Skeletal Muscle Tissue ◽  
Multiple Cell

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

Sign in / Sign up

Export Citation Format

Share Document