LC-MS analysis of p-aminosalicylic acid under electrospray ionization conditions manifests a profound solvent effect

The Analyst ◽  
2020 ◽  
Vol 145 (15) ◽  
pp. 5333-5344 ◽  
Author(s):  
Jisha Chandran ◽  
Zhaoyu Zheng ◽  
Vibin Ipe Thomas ◽  
C. Rajalakshmi ◽  
Athula B. Attygalle
Keyword(s):  
Solvent Effect ◽  
Electrospray Ionization ◽  
Mobile Phase ◽  
Mass Spectrometric ◽  
Chromatographic Peak ◽  
Aminosalicylic Acid ◽  
Ms Analysis

Under identical mass spectrometric conditions, chromatographic peak intensities of p-aminosalicylic acid recorded by LC-MS, using methanol as the mobile phase are drastically different from those acquired using is it acetonitrile as the eluent.

Comparison of the electrospray ionization (ESI) responses of penicillins with ESI responses of their methanolysis products

2018 ◽  
Vol 25 (4) ◽  
pp. 357-361
Author(s):  
Lidia Podniesińska ◽  
Rafał Frański ◽  
Magdalena Frańska
Keyword(s):  
Electrospray Ionization ◽  
Penicillin G ◽  
Chromatographic Peak ◽  
Esi Ms ◽  
Ms Analysis

The electrospray ionization (ESI) responses, defined as the area of chromatographic peak of ion [M+H]+ obtained upon HPLC/ESI-MS analysis, of three β-lactam antibiotics, namely penicillin G, ampicillin and carbenicillin have been compared with the ESI responses of their methanolysis products. It has been found that methanolyzed penicillin G has much higher ESI response than the penicillin G. Methanolyzed ampicillin also has higher ESI response than ampicillin; however, the effect is less pronounced than for penicillin. Methanolyzed carbenicillin does not have pronouncedly higher ESI response than carbenicillin.

A Modified QuEChERS Extraction and LC-MS/MS Method for the Determination of Pesticide Residues in Curry Leaves (Murraya koenigii)

2019 ◽  
Vol 6 (1) ◽  
pp. 30-41
Author(s):  
Ranjith Arimboor ◽  
Karunkara Ramakrishna Menon ◽  
Natarajan Ramesh Babu ◽  
Haneesh Chandran
Keyword(s):  
Sample Preparation ◽  
Matrix Effect ◽  
Retention Time ◽  
Pesticide Residues ◽  
Mobile Phase ◽  
Preparation Technique ◽  
Modified Quechers ◽  
Curry Leaves ◽  
Ms Analysis ◽  
The Matrix

Background:Increased consumer demand for curry leaves free from pesticides demands fast and reliable analytical methods for the analysis of pesticide residues.Objective:The optimization of a QuEChERS based sample preparation technique with improved analytical accuracy by removing interfering matrix components for LC-MS/MS analysis of pesticide residues from curry leaves.Methods:A modified QuEChERS solid phase extraction method was developed and validated for the analysis of 26 pesticides in fresh and dried curry leaves. The effects of the sample preparation steps and column retention time on the matrix suppression of analyte ions were also evaluated.Results:Validation parameters were found within an acceptable range. The matrix effect evaluation studies showed that the QuEChERS sample preparation was able to minimize the ion suppression of analytes due to co-eluting matrix of components and that a d-SPE clean up step had major role in reducing matrix effect. The gradient mobile phase with longer retention time for analytes resulted in comparatively lesser matrix effects than the isocratic mobile phase of non-polar nature. Even after the clean up, a considerable number of compounds had more than 20% reduction in their MS response in the gradient mobile phase.Conclusion:This study emphasized the need of proper sample clean up before a LC-MS/MS analysis and the usage of matrix matched standards and mobile phase that ultimately results in an appropriate analyte separation in reasonable retention times.

Gas Chromatographic/Mass Spectrometric Confirmation of Leucomalachite Green in Catfish (Ictalurus punctatus) Tissue

1995 ◽  
Vol 78 (4) ◽  
pp. 971-977 ◽  
Author(s):  
Sherri B Turnipseed ◽  
José E Roybal ◽  
Jeffrey A Hurlbut ◽  
Austin R Long
Keyword(s):  
Solid Phase Extraction ◽  
Ictalurus Punctatus ◽  
Solid Phase ◽  
Mass Spectrometric ◽  
Phase Extraction ◽  
Neutral Alumina ◽  
Ms Analysis ◽  
Solid Phase Extraction Cartridge ◽  
Leucomalachite Green ◽  
Chromatographic Mass

Abstract A gas chromatographic/mass spectrometric (GC/MS) method was developed to confirm the presence of leucomalachite green (LMG), a metabolite of the triphenylmethane dye malachite green (MG), in catfish tissue. Residues were isolated according to a previously described liquid chromatographic (LC)A/IS spectrometric analysis of MG and LMG in fish. In our isolation procedure, analytes are extracted from tissue with acetonitrile–buffer, partitioned into CH2CI2, and applied to neutral alumina and propylsulfonic acid solid-phase extraction cartridges. Before GC/MS analysis, extracts prepared for the LC determinative method are eluted from a cyano solid-phase extraction cartridge, extracted into organic solvent, and concentrated for GC/MS analysis. Selected ion monitoring was performed by using 5 diagnostic ions (m/z 330,329,253,210, and 165) of LMG. The method was validated by confirming LMG in tissue fortified with mixtures of MG and LMG (5 and 10 ng/g each) and in tissue from fish that had been exposed to low levels of MG.

Trace Determination of Glycols by HPLC with UV and Electrospray Ionization Mass Spectrometric Detections

1999 ◽  
Vol 71 (13) ◽  
pp. 2288-2293 ◽  
Author(s):  
M. Holčapek ◽  
H. Virelizier ◽  
J. Chamot-Rooke ◽  
P. Jandera ◽  
C. Moulin
Keyword(s):  
Electrospray Ionization ◽  
Mass Spectrometric ◽  
Ionization Mass ◽  
Trace Determination

Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates

2014 ◽  
Vol 28 (6) ◽  
pp. 645-652
Author(s):  
Chunying Ma ◽  
Chao Li ◽  
Xingrong Luan ◽  
Jin Zhang ◽  
Renzhong Qiao ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Mass Spectrometric ◽  
Ionization Mass ◽  
Characteristic Fragmentation

Electrospray ionization mass spectrometric analysis of phospholipids of Escherichia coli

1996 ◽  
Vol 20 (1) ◽  
pp. 233-238 ◽  
Author(s):  
G. Sweetman ◽  
M. Trinei ◽  
J. Modha ◽  
J. Kusel ◽  
P. Freestone ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Electrospray Ionization ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Ionization Mass
Sign in / Sign up

Export Citation Format

Share Document