Gold nanoparticle-streptavidin conjugates for rapid and efficient screening of aptamer function in lateral flow sensors using novel CD4-binding aptamers identified through Crossover-SELEX

The Analyst ◽  
2020 ◽  
Vol 145 (15) ◽  
pp. 5180-5193 ◽  
Author(s):  
Tamika Fellows ◽  
Lance Ho ◽  
Shane Flanagan ◽  
Ronen Fogel ◽  
Dupe Ojo ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Reporting System ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Flow Sensors ◽  
Target Proteins ◽  
Lateral Flow Tests ◽  
Efficient Screening

Adaptation of conventional lateral flow tests: demonstration of a lateral flow assay in which gold nanoparticle conjugates, when bound to aptamers, serve as a reporting system to screen novel aptamers for the ability to bind to target proteins.

A lateral flow assay for the determination of human tetanus antibody in whole blood by using gold nanoparticle labeled tetanus antigen

2018 ◽  
Vol 185 (2) ◽  
Author(s):  
Juanzu Liu ◽  
Junyan Wang ◽  
Zhaohui Li ◽  
Hongmin Meng ◽  
Lin Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Whole Blood ◽  
Lateral Flow ◽  
Lateral Flow Assay

scholarly journals A rapid and portable sensor based on protein-modified gold nanoparticle probes and lateral flow assay for naked eye detection of mercury ion

2012 ◽  
Vol 97 ◽  
pp. 294-296 ◽  
Author(s):  
Cheng-Han Chao ◽  
Chung-Shu Wu ◽  
Chung-Chih Huang ◽  
Jie-Chian Liang ◽  
Hsiao-Ting Wang ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Mercury Ion ◽  
Lateral Flow Assay ◽  
Eye Detection ◽  
Nanoparticle Probes ◽  
Naked Eye ◽  
Portable Sensor ◽  
Naked Eye Detection

scholarly journals Design of Multiplex Lateral Flow Tests: A Case Study for Simultaneous Detection of Three Antibiotics

2019 ◽  
Author(s):  
Anastasiya V. Bartosh ◽  
Dmitriy V. Sotnikov ◽  
Olga D. Hendrickson ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Optimal Location ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Immunochromatographic Test ◽  
Equilibrium Conditions ◽  
Lateral Flow Tests ◽  
The Impact

The presented study is focused on the impact of binding zones locations at immunochromatographic test strips into analytical parameters of multiplex lateral flow assay. Due to non-equilibrium conditions for such assays the duration of immune reactions influences significantly on analytical parameters, and the integration of several analytes into one multiplex strip may cause essential decrease of sensitivity. To choose the best location of binding zones, we have tested reactants for immunochromatographic assays of lincomycin, chloramphenicol, and tetracycline. The influence of the distance to the binding zones on the intensity of coloration and limit of detection (LOD) was rather different. Basing on the obtained data, the best order of binding zones was chosen. In comparison with non-optimal location the LODs were 5-10 fold improved. The final assay provides LODs 0.4, 0.4 and 1.0 ng/mL for lincomycin, chloramphenicol, and tetracycline, respectively. The proposed approach can be applied for multiassays of other analytes.

Development and evaluation of a gold nanoparticle based Lateral Flow assay (LFA) strip test for detection of Brucella spp.

2021 ◽  
pp. 106185
Author(s):  
Chandan Prakash ◽  
Bablu Kumar ◽  
Rabindra Prasad Singh ◽  
Praveen Singh ◽  
Garima Shrinet ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Strip Test

scholarly journals THE USE OF HYDROSOL HEXACIANFERRATE (II) FERRUM (III) FOR DEVELOPING DIAGNOSTIC LATERAL FLOW TESTS

2012 ◽  
Vol 67 (3) ◽  
pp. 80-85 ◽  
Author(s):  
S. N. Skopinskaya ◽  
S. P. Yarkov ◽  
V. N. Zlobin ◽  
Kh. Kh. Valiev
Keyword(s):  
Cholera Toxin ◽  
Disperse Phase ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Human Immunoglobulin ◽  
Specificity And Sensitivity ◽  
New Type ◽  
Lateral Flow Tests

On the basis of synthesized negatively charged hydrosol hexacianferrate (II) ferrum (III) (HCFF) by diameter 10–20 нм are received stable conjugates with antibodies and antigens glycoprotein and lipopolysaccharide (LPS) nature. Synthesized hydrosol (HCFF) is new type of a disperse phase in the lateral flow assay. Conjugates mentioned above were applied for construction lateral flow tests-systems for revealing cholera toxin, the rabbit antibodies to recombinant glycoproteine complex from Micobaсterium tuberculosis H37 Rv, human immunoglobulin, LPS antigens S. typhimurium, S. enteritidis. Developed lateral flow tests-systems had high analysis speed (5–7 min), good specificity and sensitivity: on cholera toxin of 2,0 ug / ml, on LPS antigens S. typhimurium, S. enteritidis 0,5 ug / ml. 

scholarly journals Signal enhancement on gold nanoparticle-based lateral flow tests using cellulose nanofibers

2019 ◽  
Vol 141 ◽  
pp. 111407 ◽  
Author(s):  
Daniel Quesada-González ◽  
Christina Stefani ◽  
Israel González ◽  
Alfredo de la Escosura-Muñiz ◽  
Neus Domingo ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Cellulose Nanofibers ◽  
Signal Enhancement ◽  
Lateral Flow ◽  
Lateral Flow Tests

Triple lines gold nanoparticle-based lateral flow assay for enhanced and simultaneous detection of Leishmania DNA and endogenous control

Nano Research ◽  
2015 ◽  
Vol 8 (11) ◽  
pp. 3704-3714 ◽  
Author(s):  
Lourdes Rivas ◽  
Alfredo de la Escosura-Muñiz ◽  
Lorena Serrano ◽  
Laura Altet ◽  
Olga Francino ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Endogenous Control

Identification and quantification of eight Listeria monocytogene serotypes from Listeria spp. using a gold nanoparticle-based lateral flow assay

2016 ◽  
Vol 184 (3) ◽  
pp. 715-724 ◽  
Author(s):  
Wenbin Wang ◽  
Liqiang Liu ◽  
Shanshan Song ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Identification And Quantification
Sign in / Sign up

Export Citation Format

Share Document