scholarly journals A novel liquid biopsy-based approach for highly specific cancer diagnostics: mitigating false responses in assaying patient plasma-derived circulating microRNAs through combined SERS and plasmon-enhanced fluorescence analyses

The Analyst ◽  
2020 ◽  
Vol 145 (12) ◽  
pp. 4173-4180 ◽  
Author(s):  
Adrianna N. Masterson ◽  
Thakshila Liyanage ◽  
Claire Berman ◽  
Hristos Kaimakliotis ◽  
Merrell Johnson ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Liquid Biopsy ◽  
Cancer Diagnostics ◽  
Enhanced Fluorescence ◽  
Circulating Micrornas ◽  
Plasmon Enhanced Fluorescence ◽  
Surface Enhanced ◽  
Specific Cancer ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Novel multimodal assay has been developed to quantify circulating microRNAs through a combined surface-enhanced Raman scattering and plasmon-enhanced fluorescence approach.

scholarly journals A 3D Plasmonic Crossed-Wire Nanostructure for Surface-Enhanced Raman Scattering and Plasmon-Enhanced Fluorescence Detection

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 281
Author(s):  
Chun-Ta Huang ◽  
Fuh-Jyh Jan ◽  
Cheng-Chung Chang
Keyword(s):  
Raman Scattering ◽  
Hot Spot ◽  
Surface Enhanced Raman Scattering ◽  
Silver Nanowire ◽  
Enhanced Fluorescence ◽  
Plasmon Enhanced Fluorescence ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Control Compound

In this manuscript, silver nanowire 3D random crossed-wire woodpile (3D-RCW) nanostructures were designed and prepared. The 3D-RCW provides rich “antenna” and “hot spot” effects that are responsive for surface-enhanced Raman scattering (SERS) effects and plasmon-enhanced fluorescence (PEF). The optimal construction mode for the 3D-RCW, based on the ratio of silver nanowire and control compound R6G, was explored and established for use in PEF and SERS analyses. We found that the RCW nanochip capable of emission and Raman-enhanced detections uses micro levels of analysis volumes. Consequently, and SERS and PEF of pesticides (thiram, carbaryl, paraquat, fipronil) were successfully measured and characterized, and their detection limits were within 5 μM~0.05 µM in 20 µL. We found that the designed 3D plasmon-enhanced platform cannot only collect the SERS of pesticides, but also enhance the fluorescence of a weak emitter (pesticides) by more than 1000-fold via excitation of the surface plasmon resonance, which can be used to extend the range of a fluorescence biosensor. More importantly, solid-state measurement using a 3D-RCW nanoplatform shows promising potential based on its dual applications in creating large SERS and PEF enhancements.

scholarly journals Surface enhanced Raman scattering of extracellular vesicles for cancer diagnostics despite isolation dependent lipoprotein contamination

Nanoscale ◽  
2021 ◽  
Author(s):  
Hanna Koster ◽  
Tatu Rojalin ◽  
Alyssa Powell ◽  
Dina Pham ◽  
Rachel Mizenko ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Extracellular Vesicles ◽  
Surface Enhanced Raman Scattering ◽  
Cancer Diagnostics ◽  
Diagnostic Utility ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Particle Counts

Given the emerging diagnostic utility of extracellular vesicles (EVs), it is important to account for non-EV contaminants. Lipoprotein present in EV-enriched isolates may inflate particle counts and decrease sensitivity to...

scholarly journals Multiplexed Liquid Biopsy and Tumor Imaging Using Surface-Enhanced Raman Scattering

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 449
Author(s):  
Francesco Dell’Olio
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Liquid Biopsy ◽  
Tumor Imaging ◽  
Surface Enhanced Raman Scattering ◽  
Detection Sensitivity ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

The recent improvements in diagnosis enabled by advances in liquid biopsy and oncological imaging significantly better cancer care. Both these complementary approaches, which are used for early tumor detection, characterization, and monitoring, can benefit from applying techniques based on surface-enhanced Raman scattering (SERS). With a detection sensitivity at the single-molecule level, SERS spectroscopy is widely used in cell and molecular biology, and its capability for the in vitro detection of several types of cancer biomarkers is well established. In the last few years, several intriguing SERS applications have emerged, including in vivo imaging for tumor targeting and the monitoring of drug release. In this paper, selected recent developments and trends in SERS applications in the field of liquid biopsy and tumor imaging are critically reviewed, with a special emphasis on results that demonstrate the clinical utility of SERS.

Nanoparticle Tracers in Reservoir-On-A-chip by Surface-Enhanced Raman Scattering - Fluorescence SERS-SEF Imaging Technology

2021 ◽  
Author(s):  
Sehoon Chang ◽  
Shannon L. Eichmann ◽  
Wei Wang
Keyword(s):  
Porous Media ◽  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Composite Nanoparticles ◽  
Dye Molecules ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Abstract Nanoparticles or nanocomposite fluids are injected into oil reservoirs for reservoir tracing or to improve injectivity or recovery of oil. Effective application of nanoparticles in fluid flooding still needs to be investigated. Dual-mode surface-enhanced Raman scattering (SERS) - surface-enhanced fluorescence (SEF) composite nanoparticles have been developed as nanoparticle reservoir tracers. This presentation discusses their transport and detectability in porous media, providing valuable information for understanding the role of nanoparticles in EOR process. The dual-mode surface-enhanced Raman scattering (SERS) - surface-enhanced fluorescence (SEF) composite nanoparticles are synthesized composed of Ag or Au metal cores, specific dye molecules, and a SiO2 shell materials. To optimize maximum signal enhancement of both phenomena such as SERS and SEF, the distance between core metal nanoparticles and dye molecules are precisely controlled. The synthesized composite nanoparticles barcoded with dye molecules are detectable by both fluorescence and Raman spectroscopies due to the SERS-SEF phenomena. Both fluorescence and Raman microscopic images of dye embedded surfaceenhanced Raman scattering (SERS) surface-enhanced fluorescence (SEF) composite nanoparticles in water phase successfully were collected within microfluidic reservoir-on-a-chip. The reservoir-on-a-chip utilized in this study fabricated based on reservoir rock geometry and coated with calcium carbonate. The synthesized SERS-SEF composite nanoparticles in water solution have been flooded into the microfluidic reservoir-on-a-chip and imaged for probing interfacial behavior of fluids such as liquid-liquid interfaces and studying the behavior of nanoparticles at liquid-rock interfaces. The precise synthesis method to produce the composite nanoparticles has been developed for the embedded dye molecules to generate noticeably enhanced detectability due to the strong SERS phenomenon. In conclusion, SERS-SEF nanoparticles barcoded with the fingerprinted Raman and fluorescence signals can provide a possible pathway toward SERS-SEF nanoprobe as various barcoded tracers to understand fluid behavior in porous media. Composite nanoparticle synthesis and its detection in flow technologies have been developed for visualization of the fluid flow behavior in porous media representing reservoir rock geometry. The results of the high-resolution nanoparticle fluid imaging data in reservoir-on-a-chip can be applied to understand mechanism of nanoparticle fluid assisted chemical enhanced oil recovery.

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