A paper-based conductive immunosensor for the determination of Salmonella Typhimurium

The Analyst ◽  
2020 ◽  
Vol 145 (13) ◽  
pp. 4637-4645 ◽  
Author(s):  
Wanida Wonsawat ◽  
Sunisa Limvongjaroen ◽  
Sathaporn Supromma ◽  
Wattana Panphut ◽  
Nipapan Ruecha ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Quantitative Method ◽  
Highly Sensitive ◽  
First Time

We report for the first time a highly sensitive and rapid quantitative method for the detection of Salmonella Typhimurium (S. Typhimurium) using a conductive immunosensor on a paper-based device (PAD).

scholarly journals Determination of Flavonoids Compounds of Three Species and Different Harvesting Periods in Crataegi folium Based on LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1602
Author(s):  
Ya-Ping Guo ◽  
Hong Yang ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Quantitative Method ◽  
Total Content ◽  
Qualitative And Quantitative Analysis ◽  
Pharmacological Activities ◽  
Time Saving ◽  
Qualitative And Quantitative ◽  
First Time

Crataegi folium have been used as medicinal and food materials worldwide due to its pharmacological activities. Although the leaves of Crataegus songorica (CS), Crataegus altaica (CA) and Crataegus kansuensis (CK) have rich resources in Xinjiang, China, they can not provide insights into edible and medicinal aspects. Few reports are available on the qualitative and quantitative analysis of flavonoids compounds of their leaves. Therefore, it is necessary to develop efficient methods to determine qualitative and quantitative flavonoids compounds in leaves of CS, CA and CK. In the study, 28 unique compounds were identified in CS versus CK by qualitative analysis. The validated quantitative method was employed to determine the content of eight flavonoids of the leaves of CS, CA and CK within 6 min. The total content of eight flavonoids was 7.8–15.1 mg/g, 0.1–9.1 mg/g and 4.8–10.7 mg/g in the leaves of CS, CA and CK respectively. Besides, the best harvesting periods of the three species were from 17th to 26th September for CS, from 30th September to 15th October for CA and CK. The validated and time-saving method was successfully implemented for the analysis of the content of eight flavonoids compounds in CS, CA and CK for the first time.

scholarly journals Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Nadja Wilhelm ◽  
Nadja Perle ◽  
Robert Simmoteit ◽  
Christian Schlensak ◽  
Hans P. Wendel ◽  
...  
Keyword(s):  
Surgical Instruments ◽  
Surgical Instrument ◽  
Quantitative Real Time Pcr ◽  
Significant Extent ◽  
Cleaning Efficiency ◽  
Established Method ◽  
Highly Sensitive ◽  
First Time ◽  
Test Objects

Surgical instruments are often strongly contaminated with patients’ blood and tissues, possibly containing pathogens. The reuse of contaminated instruments without adequate cleaning and sterilization can cause postoperative inflammation and the transmission of infectious diseases from one patient to another. Thus, based on the stringent sterility requirements, the development of highly efficient, validated cleaning processes is necessary. Here, we use for the first time synthetic single-stranded DNA (ssDNA_ODN), which does not appear in nature, as a test soiling to evaluate the cleaning efficiency of routine washing processes. Stainless steel test objects were coated with a certain amount of ssDNA_ODN. After cleaning, the amount of residual ssDNA_ODN on the test objects was determined using quantitative real-time PCR. The established method is highly specific and sensitive, with a detection limit of 20 fg, and enables the determination of the cleaning efficiency of medical cleaning processes under different conditions to obtain optimal settings for the effective cleaning and sterilization of instruments. The use of this highly sensitive method for the validation of cleaning processes can prevent, to a significant extent, the insufficient cleaning of surgical instruments and thus the transmission of pathogens to patients.

Highly sensitive electrochemical sensor for simultaneous determination of dihydroxybenzene isomers based on Co doped SnO2 nanoparticles

RSC Advances ◽  
2016 ◽  
Vol 6 (72) ◽  
pp. 68211-68219 ◽  
Author(s):  
N. Lavanya ◽  
C. Sekar
Keyword(s):  
Phenolic Compounds ◽  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Sno2 Nanoparticles ◽  
Dihydroxybenzene Isomers ◽  
Highly Sensitive ◽  
First Time ◽  
Co Doped

We have fabricated an electrochemical sensor for the simultaneous determination of two dihydroxybenzene isomers of phenolic compounds, hydroquinone (HQ) and catechol (CC) using Co doped SnO2 nanoparticles (Co-SnO2 NPs) for the first time.

Growth Response of Salmonella Typhimurium in the Presence of Natural and Synthetic Antimicrobials: Estimation of MICs from Three Different Models

2007 ◽  
Vol 70 (10) ◽  
pp. 2243-2250 ◽  
Author(s):  
L. GUILLIER ◽  
A. I. NAZER ◽  
F. DUBOIS-BRISSONNET
Keyword(s):  
Organic Acids ◽  
Salmonella Typhimurium ◽  
Aromatic Compounds ◽  
Quantitative Method ◽  
Quantitative Methods ◽  
Growth Response ◽  
Brain Heart Infusion ◽  
Brain Heart Infusion Medium ◽  
Experiment Duration

The aim of this study was to determine the MICs of 14 antimicrobials for Salmonella Typhimurium with three methods and to check the influence of experiment duration on the estimation of MICs. The growth of Salmonella Typhimurium in a brain heart infusion medium containing various concentrations of natural aromatic compounds, organic acids, or salts was monitored by absorbance measurements for 24 or 72 h. Three different ways of analyzing optical density (OD) curves were tested for the determination of MICs. Both quantitative methods gave similar MICs for most of the compounds. The semi-quantitative method does not allow estimating the MIC for all compounds. Noticeable differences were found between MICs obtained for 24- or 72-h experiments, whatever the method used. The proposed methods and models can be used for the estimation of MICs from OD data. MICs could be used for a quantitative approach to Salmonella Typhimurium growth.

Determination of antibacterial propertiesof triazines in regards to Salmonella typhimurium

2017 ◽  
Vol 19 (1) ◽  
pp. 16-23
Author(s):  
M. M. Babkina ◽  
◽  
O. V. Vasylchenko ◽  
O. M. Deriabin ◽  
A. A. Tarasov ◽  
...  
Keyword(s):  
Salmonella Typhimurium

PROFITABILITY ANALYSIS OF TERUBUK FARMING (SACCHARUM EDULE HASSKARL) IN SUBDISTRICT TOSA DISTRICT OF EAST TIDORE OF TIDORE ISLAND

2018 ◽  
Author(s):  
Suwandi S. Sangadji
Keyword(s):  
Data Collection ◽  
Research Method ◽  
Quantitative Method ◽  
Secondary Data ◽  
Primary Data ◽  
Profitability Analysis ◽  
Sample Method ◽  
Document Review ◽  
Revenue Production

The purpose of this researchment is to ascertain how wide the farming of species Saccharun Edule Hasskarl (terubuk) in sub district Tosa, district of East Tidore of Tidore Island through the indicator of the value revenue, production and selling prices so that the farmers will achieve The Break Event Point (BEP). The research method was used a quantitative method with the number of samples of 30 people. The determination of the sample method is using the census method or involving all members of the population into a sample of researchment. The secondary data collection was done by using library literature in the form of document review and relevant references to research object while primary data collection was done by using questionnaire. The data is using equation R /C Ratio, BEP Revenue, BEP Price, and BEP Production. Therefore from the results of the researchment it can be explained that the two of the thirty farmers come through the break event point, while the other twenty-eight farmers declared having a business that worth to be develop or experiencing profit, because the R/C ratio is above 1.0 with average profit reach Rp. 989.000, - per production / farmer.

Disability as a social phenomenon

2020 ◽  
pp. 22-38
Author(s):  
Natalia Guseva ◽  
Vitaliy Berdutin
Keyword(s):  
Social Protection ◽  
Russian Society ◽  
Stage Model ◽  
High Quality ◽  
Rehabilitation Programs ◽  
Current State ◽  
The Social ◽  
Consistent Solution ◽  
First Time

At present, the problem of establishing disability is a point at issue in Russia. Despite the fact that medical criteria for disability are being developed very actively, high-quality methods for assessing social hallmarks are still lacking. Since disability is a phenomenon inherent in any society, each state forms a social and economic policy for people with disabilities in accordance with its level of development, priorities and opportunities. We have proposed a three-stage model, which includes a system for the consistent solution of the main tasks aimed at studying the causes and consequences of the problems encountered today in the social protection of citizens with health problems. The article shows why the existing approaches to the determination of disability and rehabilitation programs do not correspond to the current state of Russian society and why a decrease in the rate of persons recognized as disabled for the first time does not indicate an improvement in the health of the population. The authors proposed a number of measures with a view to correcting the situation according to the results of the study.

Controlled Growth and Characterization Ag/ZnO Nanotetrapods for Humidity Sensing

2018 ◽  
Vol 21 (7) ◽  
pp. 462-467
Author(s):  
Babak Sadeghi
Keyword(s):  
Room Temperature ◽  
Zinc Complex ◽  
Water Solution ◽  
Particle Morphology ◽  
Quick Response ◽  
Separation System ◽  
Humidity Sensing ◽  
Sensor Material ◽  
Highly Sensitive

Aim and Objective: Ultrafine Ag/ZnO nanotetrapods (AZNTP) have been prepared successfully using silver (I)–bis (oxalato) zinc complex and 1, 3-diaminopropane (DAP) with a phase separation system, and have been injected into a diethyl/water solution. Materials and Methods: This crystal structure and lattice constant of the AZNTP obtained were investigated by means of a SEM, XRD, TEM and UV-vis spectrum. Results: The results of the present study demonstrated the growth and characterization AZNTP for humidity sensing and DAP plays a key role in the determination of particle morphology. AZNTP films with 23 nm in arm diameter have shown highly sensitive, quick response sensor material that works at room temperature.

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