scholarly journals Microfluidic perfusion modulates growth and motor neuron differentiation of stem cell aggregates

The Analyst ◽  
2020 ◽  
Vol 145 (14) ◽  
pp. 4815-4826 ◽  
Author(s):  
Emily L. Jackson-Holmes ◽  
Amanda W. Schaefer ◽  
Todd C. McDevitt ◽  
Hang Lu
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Motor Neuron ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Cell Aggregates ◽  
Neuron Differentiation ◽  
Exchange Frequency ◽  
Microfluidic Perfusion ◽  
Device Geometry

This work explores how media exchange frequency and device geometry modulate the biochemical environment and impact three-dimensional stem cell differentiation.

Evaluation of a Thermoresponsive Polycaprolactone Scaffold for In Vitro Three-Dimensional Stem Cell Differentiation

2015 ◽  
Vol 21 (1-2) ◽  
pp. 310-319 ◽  
Author(s):  
Veronika Hruschka ◽  
Aram Saeed ◽  
Paul Slezak ◽  
Racha Cheikh Al Ghanami ◽  
Georg Alexander Feichtinger ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Three Dimensional ◽  
Stem Cell Differentiation

scholarly journals Induced Pluripotent Stem Cell Differentiation and Three-Dimensional Tissue Formation Attenuate Clonal Epigenetic Differences in Trichohyalin

2016 ◽  
Vol 25 (18) ◽  
pp. 1366-1375 ◽  
Author(s):  
Anastasia Petrova ◽  
Antonio Capalbo ◽  
Laureen Jacquet ◽  
Simon Hazelwood-Smith ◽  
Dimitra Dafou ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Pluripotent Stem Cell ◽  
Three Dimensional ◽  
Induced Pluripotent Stem Cell ◽  
Stem Cell Differentiation ◽  
Tissue Formation ◽  
Induced Pluripotent

scholarly journals Glycosaminoglycans as regulators of stem cell differentiation

2011 ◽  
Vol 39 (1) ◽  
pp. 383-387 ◽  
Author(s):  
Raymond A.A. Smith ◽  
Kate Meade ◽  
Claire E. Pickford ◽  
Rebecca J. Holley ◽  
Catherine L.R. Merry
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Es Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Cell Types ◽  
Specific Pattern ◽  
Embryonic Stem Cell Differentiation ◽  
Specific Cell

ES (embryonic stem) cell differentiation is dependent on the presence of HS (heparan sulfate). We have demonstrated that, during differentiation, the evolution of specific cell lineages is associated with particular patterns of GAG (glycosaminoglycan) expression. For example, different HS epitopes are synthesized during neural or mesodermal lineage formation. Cell lines mutant for various components of the HS biosynthetic pathway are selectively impaired in their differentiation, with lineage-specific effects observed for some lines. We have also observed that the addition of soluble GAG saccharides to cells, with or without cell-surface HS, can influence the pace and outcome of differentiation, again highlighting specific pattern requirements for particular lineages. We are combining this work with ongoing studies into the design of artificial cell environments where we have optimized three-dimensional scaffolds, generated by electrospinning or by the formation of hydrogels, for the culture of ES cells. By permeating these scaffolds with defined GAG oligosaccharides, we intend to control the mechanical environment of the cells (via the scaffold architecture) as well as their biological signalling environment (using the oligosaccharides). We predict that this will allow us to control ES cell pluripotency and differentiation in a three-dimensional setting, allowing the generation of differentiated cell types for use in drug discovery/testing or in therapeutics.

scholarly journals Three dimensional cellular microarray platform for human neural stem cell differentiation and toxicology

2014 ◽  
Vol 13 (1) ◽  
pp. 36-47 ◽  
Author(s):  
Luciana Meli ◽  
Hélder S.C. Barbosa ◽  
Anne Marie Hickey ◽  
Leyla Gasimli ◽  
Gregory Nierode ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Neural Stem Cell ◽  
Three Dimensional ◽  
Microarray Platform ◽  
Stem Cell Differentiation ◽  
Human Neural Stem Cell

Three-dimensional piezoelectric fibrous scaffolds selectively promote mesenchymal stem cell differentiation

Biomaterials ◽  
2017 ◽  
Vol 149 ◽  
pp. 51-62 ◽  
Author(s):  
Sita M. Damaraju ◽  
Yueyang Shen ◽  
Ezinwa Elele ◽  
Boris Khusid ◽  
Ahmad Eshghinejad ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Fibrous Scaffolds ◽  
Mesenchymal Stem Cell Differentiation
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