In situ amplified photothermal immunoassay for neuron-specific enolase with enhanced sensitivity using Prussian blue nanoparticle-loaded liposomes

The Analyst ◽  
2020 ◽  
Vol 145 (12) ◽  
pp. 4164-4172 ◽  
Author(s):  
Li-Juan Zhi ◽  
Ai-Li Sun ◽  
Dianping Tang
Keyword(s):  
Prussian Blue ◽  
Neuron Specific Enolase ◽  
Enhanced Sensitivity ◽  
Prussian Blue Nanoparticles ◽  
Low Sensitivity

Methods based on prussian blue nanoparticles (PBNPs) have been reported for photothermal immunoassays in analytical nanoscience fields but most suffer from low sensitivity and are not beneficial for routine use.

scholarly journals Nanoporous Iron Oxide/Carbon Composites through In-Situ Deposition of Prussian Blue Nanoparticles on Graphene Oxide Nanosheets and Subsequent Thermal Treatment for Supercapacitor Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 776 ◽  
Author(s):  
Alowasheeir Azhar ◽  
Yusuke Yamauchi ◽  
Abeer Enaiet Allah ◽  
Zeid A. Alothman ◽  
Ahmad Yacine Badjah ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Iron Oxide ◽  
Thermal Treatment ◽  
Prussian Blue ◽  
High Current Density ◽  
Carbon Composite ◽  
Hybrid Structure ◽  
Carbon Composites ◽  
Prussian Blue Nanoparticles

This work reports the successful preparation of nanoporous iron oxide/carbon composites through the in-situ growth of Prussian blue (PB) nanoparticles on the surface of graphene oxide (GO) nanosheets. The applied thermal treatment allows the conversion of PB nanoparticles into iron oxide (Fe2O3) nanoparticles. The resulting iron oxide/carbon composite exhibits higher specific capacitance at all scan rates than pure GO and Fe2O3 electrodes due to the synergistic contribution of electric double-layer capacitance from GO and pseudocapacitance from Fe2O3. Notably, even at a high current density of 20 A g−1, the iron oxide/carbon composite still shows a high capacitance retention of 51%, indicating that the hybrid structure provides a highly accessible path for diffusion of electrolyte ions.

A simple thermometer-based photothermometric assay for alkaline phosphatase activity based on target-induced nanoprobe generation

2020 ◽  
Vol 44 (41) ◽  
pp. 17753-17760
Author(s):  
Honghong Rao ◽  
Huiyi Huang ◽  
Xinyuan Zhang ◽  
Xin Xue ◽  
Mingyue Luo ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Prussian Blue ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Prussian Blue Nanoparticles

Alkaline phosphatase (ALP)-induced in situ generation of Prussian blue nanoparticles for photothermometric ALP detection.

scholarly journals Enhanced sensitivity for light and electron microscopic in situ hybridization with multiple simultaneous non-radioactive oligodeoxynucleotide probes.

1995 ◽  
Vol 43 (8) ◽  
pp. 829-841 ◽  
Author(s):  
A Trembleau ◽  
F E Bloom
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent Dyes ◽  
General Procedure ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Enhanced Sensitivity ◽  
Low Sensitivity

Although oligonucleotide probes are useful for in situ hybridization, their low sensitivity compared to riboprobes and cDNA remains a problem. We have systematically examined the protocols to provide a general procedure that increases the sensitivity of oligoprobes for light and electron in situ hybridizations by using mixtures of multiple non-overlapping oligonucleotides (multi-oligoprobes). The protocol achieves these improvements with both radioactive and non-radioactive oligoprobes. With 33P-labeled probes in a semiquantitative assay, we found that mixtures of up to six vasopressin-directed multi-oligoprobes, each employed at saturating concentration, led to an additive signal with no significant increase of the background. Using this approach with non-radioactive oligoprobes, we were able to detect in the hypothalamus several low or moderately abundant mRNAs, such as vasopressin heterogeneous nuclear RNA and the galanin, dynorphin, and tyrosine hydroxylase mRNAs. Moreover, we showed that multi-oligoprobes used in a pre-embedding procedure were suitable for studying the ultrastructural compartmentalization of moderately abundant mRNAs. Finally, with the same basic approach we demonstrated that two sets of multi-oligoprobes can be combined for simultaneous detection of two different mRNAs using fluorescent dyes, making this approach suitable for high-resolution confocal analyses. Overall, our data demonstrate that multi-oligoprobes provide a sensitive tool of choice for various applications in which both well-preserved morphology and high sensitivity are needed. In particular, these probes appear ideal for study of the comparative subcellular localization of mRNAs at both the light and the electron microscopic level.

In situ synthesis of Prussian blue nanoparticles within a biocompatible reverse micellar system for in vivo Cs+uptake

2017 ◽  
Vol 41 (8) ◽  
pp. 2887-2890 ◽  
Author(s):  
Cyril Lavaud ◽  
Marilyn Kajdan ◽  
Elsa Compte ◽  
Jean-Claude Maurel ◽  
Joséphine Lai Kee Him ◽  
...  
Keyword(s):  
Prussian Blue ◽  
High Efficiency ◽  
In Situ Synthesis ◽  
Dose Effect ◽  
Micellar System ◽  
Prussian Blue Nanoparticles ◽  
Reverse Micellar System ◽  
Reverse Micellar

A Prussian blue reverse micellar system forin vivoCs+uptake exhibiting high efficiency and a significant dose effect.

Glucoamylase-labeled nanogold flowers for in situ enhanced sensitivity of a glucometer-based enzyme immunoassay

2015 ◽  
Vol 7 (2) ◽  
pp. 507-512 ◽  
Author(s):  
Xiaohong Fu ◽  
Kun Xu ◽  
Jun Ye ◽  
Jie Chen ◽  
Xueyu Feng
Keyword(s):  
Enzyme Immunoassay ◽  
Enhanced Sensitivity ◽  
Low Sensitivity ◽  
Enzyme Labels

Methods based on enzyme labels have been developed for glucometer-based immunoassays, but most involve low sensitivity and are unsuitable for routine use.

Prussian blue nanoparticles encapsulated inside a metal–organic framework via in situ growth as promising peroxidase mimetics for enzyme inhibitor screening

2016 ◽  
Vol 4 (1) ◽  
pp. 128-134 ◽  
Author(s):  
Linjing Su ◽  
Yuhao Xiong ◽  
Haiguan Yang ◽  
Peng Zhang ◽  
Fanggui Ye
Keyword(s):  
Prussian Blue ◽  
Enzyme Inhibitor ◽  
Metal Organic Framework ◽  
In Situ Growth ◽  
Inhibitor Screening ◽  
Prussian Blue Nanoparticles ◽  
Metal Organic ◽  
Organic Framework

Metal–organic framework-based peroxidase mimetics for enzyme-inhibitor screening.

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