Improving the throughput of immunoaffinity purification and enzymatic digestion of therapeutic proteins using membrane-immobilized reagent technology

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 3148-3156
Author(s):  
Michelle R. Robinson ◽  
Lisa A. Vasicek ◽  
Christian Hoppmann ◽  
Mandy Li ◽  
Gia Jokhadze ◽  
...  
Keyword(s):  
Therapeutic Proteins ◽  
Enzymatic Digestion ◽  
Membrane Technology ◽  
Immunoaffinity Purification ◽  
Immobilized Reagent

Rapid spin membrane technology decreases the time for IP and digestion of therapeutic proteins.

Confirmation of Long Term Excreted Metabolites of Stanozolol by Gas Chromatography Coupled with High Resolution Mass Spectrometry (GC/HRMS)

2008 ◽  
Vol 59 (7) ◽  
Author(s):  
Ileana Vajiala ◽  
Ruxandra Subasu ◽  
Mirela Zorio ◽  
Rodica Picu
Keyword(s):  
High Resolution Mass Spectrometry ◽  
Parent Compound ◽  
Urine Specimen ◽  
Decision Making Process ◽  
Purification Procedure ◽  
Specific Identification ◽  
Immunoaffinity Purification ◽  
In Doping ◽  
Resolution Mass

Upon screening identification of Stanozolol, GC/HRMS confirmation of the suspicious sample is done by reanalysis of the urine specimen, where a specific immunoaffinity purification procedure is used to selectively isolate the long term excreted metabolites of Stanozolol. By meeting the specific identification criteria for more than one metabolite of the same parent compound, additional evidence could be obtained in the decision making process in doping control.

Application of membrane technology in the pretreatment of cheese dairies wastes and co-treatment in a municipal conventional biological unit

1997 ◽  
Vol 36 (2-3) ◽  
pp. 361-367 ◽  
Author(s):  
Eleftheria Papachristou ◽  
Costas T. Lafazanis
Keyword(s):  
Cheese Whey ◽  
Treatment Plant ◽  
Membrane Technology ◽  
Biological Unit ◽  
Urban Sewage ◽  
Dairy Wastes ◽  
Starting Point ◽  
Quality Of Milk ◽  
Cheese Production

A great number of cheese dairies and dairy industries in Greece are disposing their wastes, mainly cheese whey, either on land or in surface receivers, in large quantities creating a major environmental problem. A typical agricultural and pastoral provincial town of 70,000 inhabitants, Trikala, became the starting point of this research. A co-treatment of the urban sewage and the dairy wastes in the municipal treatment plant was recommended. The successful application of the above statement is based primarily on the pretreatment of the cheese dairies wastes. So far for cheese whey the recovery of the lactose serum in the contemporary central unit applying membrane technology has been suggested. As far as the wastewaters of the washing and refrigeration are concerned a pretreatment is required for the defatting in a grease trap, the grating, the adjustment of pH and the equalisation in an appropriate tank. Finally, this research has also focussed on the importance of membrane technology in improving the quality of milk and cheese production.

Recent Updates on Molecular Genetic Engineering Approaches and Applications of Human Therapeutic Proteins

2017 ◽  
Vol 18 (3) ◽  
pp. 217-232 ◽  
Author(s):  
Asim Azhar ◽  
Ejaj Ahmad ◽  
Qamar Zia ◽  
Mohammad Owais ◽  
Ghulam Md Ashraf
Keyword(s):  
Genetic Engineering ◽  
Molecular Genetic ◽  
Therapeutic Proteins
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