Delivering siRNA to control osteogenic differentiation and real-time detection of cell differentiation in human mesenchymal stem cells using multifunctional gold nanoparticles

2020 ◽  
Vol 8 (15) ◽  
pp. 3016-3027 ◽  
Author(s):  
Qian Wu ◽  
Kaipeng Wang ◽  
Xichao Wang ◽  
Guohai Liang ◽  
Jinming Li
Keyword(s):  
Stem Cells ◽  
Gold Nanoparticles ◽  
Mesenchymal Stem Cells ◽  
Cell Differentiation ◽  
Osteogenic Differentiation ◽  
Real Time ◽  
Human Mesenchymal Stem Cells ◽  
Schematic Representation ◽  
Real Time Detection

Schematic representation of the multifunctional gold nanoparticles (AuNP-PEI-peptide-FITC) synthesis and siRNA adsorption to silence the PPARγ gene for controlling osteogenic differentiation and real-time detection of ongoing cell differentiation in hMSCs.

scholarly journals Correction: Delivering siRNA to control osteogenic differentiation and real-time detection of cell differentiation in human mesenchymal stem cells using multifunctional gold nanoparticles

2020 ◽  
Vol 8 (25) ◽  
pp. 5545-5546
Author(s):  
Qian Wu ◽  
Kaipeng Wang ◽  
Xichao Wang ◽  
Guohai Liang ◽  
Jinming Li
Keyword(s):  
Stem Cells ◽  
Gold Nanoparticles ◽  
Mesenchymal Stem Cells ◽  
Cell Differentiation ◽  
Osteogenic Differentiation ◽  
Real Time ◽  
Human Mesenchymal Stem Cells ◽  
Real Time Detection

Correction for ‘Delivering siRNA to control osteogenic differentiation and real-time detection of cell differentiation in human mesenchymal stem cells using multifunctional gold nanoparticles’ by Qian Wu et al., J. Mater. Chem. B, 2020, 8, 3016–3027, DOI: 10.1039/c9tb02899d.

Near-infrared control and real-time detection of osteogenic differentiation in mesenchymal stem cells by multifunctional upconversion nanoparticles

Nanoscale ◽  
2020 ◽  
Vol 12 (18) ◽  
pp. 10106-10116 ◽  
Author(s):  
Kaipeng Wang ◽  
Qian Wu ◽  
Xichao Wang ◽  
Guohai Liang ◽  
Anli Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Real Time ◽  
Near Infrared ◽  
Infrared Light ◽  
Upconversion Nanoparticles ◽  
Near Infrared Light ◽  
Regeneration Medicine ◽  
Real Time Detection

A multifuctional platform based on meseporous sillicon coated upconversion nanoparticles was developed for near-infrared light controlled and real-time detection of differentiation in mesenchymal stem cells for regeneration medicine.

scholarly journals Correction: Near-infrared control and real-time detection of osteogenic differentiation in mesenchymal stem cells by multifunctional upconversion nanoparticles

Nanoscale ◽  
2020 ◽  
Vol 12 (25) ◽  
pp. 13840-13840
Author(s):  
Kaipeng Wang ◽  
Qian Wu ◽  
Xichao Wang ◽  
Guohai Liang ◽  
Anli Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Real Time ◽  
Near Infrared ◽  
Upconversion Nanoparticles ◽  
Real Time Detection

Correction for ‘Near-infrared control and real-time detection of osteogenic differentiation in mesenchymal stem cells by multifunctional upconversion nanoparticles’ by Kaipeng Wang et al., Nanoscale, 2020, 12, 10106–10116, DOI: 10.1039/D0NR00872A.

Wnt7a Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells

2019 ◽  
Author(s):  
Leiluo Yang ◽  
Qing Li ◽  
Junhong Zhang ◽  
Pengcheng Li ◽  
Chaoliang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells

scholarly journals Fluid Flow Mechanical Stimulation-Assisted Cartridge Device for the Osteogenic Differentiation of Human Mesenchymal Stem Cells

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 927
Author(s):  
Ki-Taek Lim ◽  
Dinesh-K. Patel ◽  
Sayan-Deb Dutta ◽  
Keya Ganguly
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fluid Flow ◽  
Osteogenic Differentiation ◽  
Flow Condition ◽  
Cultured Cells ◽  
Human Mesenchymal Stem Cells ◽  
Proliferation And Differentiation ◽  
Static Conditions

Human mesenchymal stem cells (hMSCs) have the potential to differentiate into different types of mesodermal tissues. In vitro proliferation and differentiation of hMSCs are necessary for bone regeneration in tissue engineering. The present study aimed to design and develop a fluid flow mechanically-assisted cartridge device to enhance the osteogenic differentiation of hMSCs. We used the fluorescence-activated cell-sorting method to analyze the multipotent properties of hMSCs and found that the cultured cells retained their stemness potential. We also evaluated the cell viabilities of the cultured cells via water-soluble tetrazolium salt 1 (WST-1) assay under different rates of flow (0.035, 0.21, and 0.35 mL/min) and static conditions and found that the cell growth rate was approximately 12% higher in the 0.035 mL/min flow condition than the other conditions. Moreover, the cultured cells were healthy and adhered properly to the culture substrate. Enhanced mineralization and alkaline phosphatase activity were also observed under different perfusion conditions compared to the static conditions, indicating that the applied conditions play important roles in the proliferation and differentiation of hMSCs. Furthermore, we determined the expression levels of osteogenesis-related genes, including the runt-related protein 2 (Runx2), collagen type I (Col1), osteopontin (OPN), and osteocalcin (OCN), under various perfusion vis-à-vis static conditions and found that they were significantly affected by the applied conditions. Furthermore, the fluorescence intensities of OCN and OPN osteogenic gene markers were found to be enhanced in the 0.035 mL/min flow condition compared to the control, indicating that it was a suitable condition for osteogenic differentiation. Taken together, the findings of this study reveal that the developed cartridge device promotes the proliferation and differentiation of hMSCs and can potentially be used in the field of tissue engineering.

Controlled Osteogenic Differentiation of Human Mesenchymal Stem Cells Using Dexamethasone-Loaded Light-Responsive Microgels

2021 ◽  
Vol 13 (6) ◽  
pp. 7051-7059
Author(s):  
Yingnan Zhang ◽  
Changhao Fang ◽  
Shuce Zhang ◽  
Robert E. Campbell ◽  
Michael J. Serpe
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells
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