Biomimetic mineralizable collagen hydrogels for dynamic bone matrix formation to promote osteogenesis

2020 ◽  
Vol 8 (15) ◽  
pp. 3064-3075 ◽  
Author(s):  
Lu Chen ◽  
Chengheng Wu ◽  
Suping Chen ◽  
Yusheng Zhang ◽  
Amin Liu ◽  
...  
Keyword(s):  
Bone Matrix ◽  
Collagen Hydrogels ◽  
Mineralizing Process

Biomimetic mineralizable collagen hydrogels simulate the dynamic bone matrix mineralizing process via the synergy of cellular secretions, and form a microenvironment that promotes osteogenesis.

Novel methods for demonstrating osseointegration of hydroxylapatite-coated titanium hip prostheses

1991 ◽  
Vol 49 ◽  
pp. 34-35 ◽  
Author(s):  
P. Frayssinet ◽  
J. Hanker ◽  
D. Hardy ◽  
B. Giammara
Keyword(s):  
Hip Prosthesis ◽  
Ethanol Concentration ◽  
Bone Matrix ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Hip Prostheses ◽  
Cellular Inclusions ◽  
Microscopic Studies ◽  
Diamond Saw ◽  
Plasma Sprayed

Prostheses implanted in hard tissues cannot be processed for electron microscopic examination or microanalysis in the same way as those in other tissues. For these reasons, we have developed methods allowing light and electron microscopic studies as well as microanalysis of the interface between bone and a metal biomaterial coated by plasma-sprayed hydroxylapatite(HA) ceramic.An HA-coated titanium hip prosthesis (Corail, Landos, France), which had been implanted for two years, was removed after death (unrelated to the orthopaedic problem). After fixation it was dehydrated in solutions of increasing ethanol concentration prior to embedment in polymethylmethacrylate(PMMA). Transverse femur sections were obtained with a diamond saw and the sections then carefully ground to a thickness of 200 microns. Plastic-embedded sections were stained for calcium with a silver methenamine modification of the von Kossa method for calcium staining and coated by carbon. They have been examined by back-scatter SEM on an ISI-SS60 operated at 25 KV. EDAX has been done on cellular inclusions and extracellular bone matrix.

Collagen XV as a bone matrix organizer

2013 ◽  
Author(s):  
David Vicente ◽  
Mikko Finnila ◽  
Valerio Izzi ◽  
Jarkko Koivunen ◽  
Taina Pihlajaniemi
Keyword(s):  
Bone Matrix ◽  
Collagen Xv

Rescue diet restores bone matrix mineralization in mice with a non-functioning vitamin D receptor

2019 ◽  
Author(s):  
Barbara Misof ◽  
Stephane Blouin ◽  
Markus Hartmann ◽  
Jochen Hofstaetter ◽  
Klaus Klaushofer ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Bone Matrix ◽  
Matrix Mineralization ◽  
Bone Matrix Mineralization

Irradiation-sterilization of rat bone matrix gelatin

1988 ◽  
Vol 59 (2) ◽  
pp. 165-167 ◽  
Author(s):  
Nikolaus Schwarz ◽  
Heinz Redl ◽  
Anna Schiesser ◽  
Gunther Schlag ◽  
Martin Thurnher ◽  
...  
Keyword(s):  
Bone Matrix ◽  
Rat Bone

THE EFFECT OF GAMMA STERILIZATION AND ITS EFFECT ON MICROSCOPIC STRUCTURE OF DEEP FROZEN DOG SKULL

2018 ◽  
pp. 37-40
Author(s):  
Phuong Thao Tien Nguyen ◽  
Anh Hung Tran ◽  
Van Tam Le ◽  
Huu Thao Phung ◽  
Phan Quynh Anh Nguyen
Keyword(s):  
Structural Change ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Key Words ◽  
Bone Matrix ◽  
Infected Tissue ◽  
Microscopic Structure ◽  
Bacteriological Examination ◽  
Microscopic Scale

Objective: To evaluate the effect of gamma sterilization and its effect on microscopic structure of deeply frozen dog skull. Subjects and methods: 50 pairs of dog skull fragments have the same size,which were taken symmetrically through the midline of the skull, were divided into two lots. Evident lot consisting of 50 pieces are just deeply frozen, not irradicated. Experimental lot which was composed of 50 pieces were deeply frozen and exposed to 25kGy of gamma radiation. Afterwards, to evaluate the effect of gamma sterilization and compare the alter of bone matrix between lot groups. Results: After gamma irradiation, all dog skull fragments were negative with bacteriological examination. In microscopic scale, no difference was found between the two lots and there was no structural change in the irradiated fragments of all dog skull. Conclusion: Gamma irradiation with 25 kGy: (i) It has the ability to kill bacteria completely in the type of bacteria commonly infected tissue. (ii) It does not alter the structure of dog skull in microstructures. Key words: dog skull, gamma, sterilize

Collagen-the Ultrastructural Element of the Bone Matrix

2018 ◽  
Vol 69 (7) ◽  
pp. 1706-1709
Author(s):  
Nicoleta Dumitru ◽  
Andra Cocolos ◽  
Andra Caragheorgheopol ◽  
Constantin Dumitrache ◽  
Ovidiu Gabriel Bratu ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Bone Microarchitecture ◽  
Complex Structure ◽  
Bone Matrix ◽  
Organic Matrix ◽  
Bone Diseases ◽  
Bone Collagen ◽  
Type I ◽  
New Therapeutic Approaches ◽  
And Function

There is an increased interest and more studies highlight the fact that bone strength depends not only on bone tissue quantity, but also on its quality, which is characterized by the geometry and shape of bones, trabecular bone microarchitecture, mineral content, organic matrix and bone turnover. Fibrillar type I collagen is the major organic component of bone matrix, providing form and a stable template for mineralization. The biomedical importance of collagen as a biomaterial for medical and cosmetic purposes and the improvement of the molecular, cellular biology and analytical technologies, led to increasing interest in establishing the structure of this protein and in setting of the relationships between sequence, structure, and function. Bone collagen crosslinking chemistry and its molecular packing structure are considered to be distinct features. This unique post-translational modifications provide to the fibrillar collagen matrices properties such as tensile strength and viscoelasticity. Understanding the complex structure of bone type I collagen as well as the dynamic nature of bone tissues will help to manage new therapeutic approaches to bone diseases.

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