Advances in functional nucleic acid based paper sensors

2020 ◽  
Vol 8 (16) ◽  
pp. 3213-3230
Author(s):  
Rudi Liu ◽  
Erin M. McConnell ◽  
Jiuxing Li ◽  
Yingfu Li
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Dna Aptamers ◽  
Extensive Review ◽  
Recognition Elements ◽  
Functional Nucleic Acids

This article provides an extensive review of paper-based sensors that utilize functional nucleic acids, particularly DNA aptamers and DNAzymes, as recognition elements.

scholarly journals Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications

2015 ◽  
Vol 2015 ◽  
pp. 1-31 ◽  
Author(s):  
Ka Lok Hong ◽  
Letha J. Sooter
Keyword(s):  
Nucleic Acid ◽  
Molecular Recognition ◽  
Nucleic Acids ◽  
Antibody Fragments ◽  
Small Peptides ◽  
Basic Principles ◽  
Single Stranded Dna ◽  
High Affinity ◽  
Ssdna Aptamer ◽  
Recognition Elements

Molecular recognition elements (MREs) can be short sequences of single-stranded DNA, RNA, small peptides, or antibody fragments. They can bind to user-defined targets with high affinity and specificity. There has been an increasing interest in the identification and application of nucleic acid molecular recognition elements, commonly known as aptamers, since they were first described in 1990 by the Gold and Szostak laboratories. A large number of target specific nucleic acids MREs and their applications are currently in the literature. This review first describes the general methodologies used in identifying single-stranded DNA (ssDNA) aptamers. It then summarizes advancements in the identification and biosensing application of ssDNA aptamers specific for bacteria, viruses, their associated molecules, and selected chemical toxins. Lastly, an overview of the basic principles of ssDNA aptamer-based biosensors is discussed.

Clinical Trials of Functional Nucleic Acids

2018 ◽  
Vol 7 (2) ◽  
pp. 46-60 ◽  
Author(s):  
Martina Traykovska ◽  
Sjoerd Miedema ◽  
Robert Penchovsky
Keyword(s):  
Clinical Trials ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Pharmaceutical Companies ◽  
Nucleic Acid Therapeutics ◽  
Drug Candidates ◽  
Safety Risks ◽  
Functional Nucleic Acids ◽  
Administration Methods

This chapter describes how functional nucleic acids, such as aptamers, antisense oligonucleotides (ASOs), small interfering (si) RNAs, and ribozymes are considered by some researchers as valuable tools to develop therapeutic agents. They have not been particularly fast in reaching the market as medicines, due to endogenous barriers to extracellular trafficking and cellular uptake of nucleic acids and their inherent instability when applied in vivo. However, research carried out by the nucleic acid engineering community and pharmaceutical companies to circumvent these obstacles has led to the approval of a few aptamers and ASOs as drugs. Nucleic acid therapeutics are usually administered locally to diseased tissue. The drug candidates currently in clinical trials commonly use the same administration methods as previously licensed nucleic acid therapeutics. These administration techniques carry their own safety risks and advantages. In this article, the present state is discussed and prospective options for the use ASOs and aptamers as drugs are listed.

scholarly journals Facile Construction of a Solely-DNA-Based System for Targeted Delivery of Nucleic Acids

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1967
Author(s):  
Ziwen Dai ◽  
Juan Li ◽  
Yongfang Lin ◽  
Zhigang Wang ◽  
Yang Huang
Keyword(s):  
Drug Delivery ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Drug Delivery System ◽  
Delivery System ◽  
Targeted Delivery ◽  
Dna Aptamers ◽  
Dna Nanostructures ◽  
Dna Strands ◽  
Targeting Ability

We designed a functional drug delivery system based solely on DNA. The whole system was built with only four DNA strands. Cyclization of DNA strands excluded the formation of byproducts. DNA aptamers were equipped to endow triangular DNA nanostructures with targeting ability. The homogeneity of materials enabled not only facile construction but also convenient loading of nucleic acid-based drugs with much ease.

Sign in / Sign up

Export Citation Format

Share Document