Beyond Fmoc: a review of aromatic peptide capping groups

2020 ◽  
Vol 8 (5) ◽  
pp. 863-877 ◽  
Author(s):  
Adam D. Martin ◽  
Pall Thordarson
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Structure And Function ◽  
Short Peptides ◽  
Peptide Structure ◽  
Energy Materials ◽  
Self Assembling ◽  
And Function

Self-assembling short peptides have widespread applications in energy materials, tissue engineering, sensing and drug delivery. In this review we discuss the effect of functional N-terminal capping groups on peptide structure and function.

scholarly journals Monoclonal Antibodies Generated against Glycoconjugates Recognize Chemical Linkers

Antibodies ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 48
Author(s):  
Jessica Ramadhin ◽  
Vanessa Silva-Moraes ◽  
Thomas Norberg ◽  
Donald Harn
Keyword(s):  
Drug Delivery ◽  
Flow Cytometry ◽  
Monoclonal Antibodies ◽  
Structure And Function ◽  
Enzyme Linked Immunosorbent Assay ◽  
Incomplete Freund’S Adjuvant ◽  
Delivery Platform ◽  
Igg1 Mabs ◽  
Human Milk Oligosaccharide ◽  
And Function

Monoclonal antibodies (mAbs) that recognize glycans are useful tools to assess carbohydrates’ structure and function. We sought to produce IgG mAbs to the human milk oligosaccharide (HMO), lacto-N-fucopentaose III (LNFPIII). LNFPIII contains the Lewisx antigen, which is found on the surface of schistosome parasites. mAbs binding the Lewisx antigen are well-reported in the literature, but mAbs recognizing HMO structures are rare. To generate mAbs, mice were immunized with LNFPIII-DEX (P3DEX) plus CpGs in VacSIM®, a novel vaccine/drug delivery platform. Mice were boosted with LNFPIII-HSA (P3HSA) plus CpGs in Incomplete Freund’s Adjuvant (IFA). Splenocytes from immunized mice were used to generate hybridomas and were screened against LNFPIII conjugates via enzyme-linked immunosorbent assay (ELISA). Three positive hybridomas were expanded, and one hybridoma, producing IgG and IgM antibodies, was cloned via flow cytometry. Clone F1P2H4D8D5 was selected because it produced IgG1 mAbs, but rescreening unexpectedly showed binding to both LNFPIII and lacto-N-neotetraose (LNnT) conjugates. To further assess the specificity of the mAb, we screened it on two glycan microarrays and found no significant binding. This finding suggests that the mAb binds to the acetylphenylenediamine (APD) linker-spacer structure of the conjugate. We present the results herein, suggesting that our new mAb could be a useful probe for conjugates using similar linker spacer structures.

Self-Assembling Peptides: Implications for Patenting in Drug Delivery and Tissue Engineering

2011 ◽  
Vol 5 (1) ◽  
pp. 24-51 ◽  
Author(s):  
Pradeep Kumar ◽  
Viness Pillay ◽  
Girish Modi ◽  
Yahya E. Choonara ◽  
Lisa C. du Toit ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Self Assembling

scholarly journals A Rapid Library Screen for Tailoring β-Peptide Structure and Function

2005 ◽  
Vol 127 (42) ◽  
pp. 14584-14585 ◽  
Author(s):  
Joshua A. Kritzer ◽  
Nathan W. Luedtke ◽  
Elizabeth A. Harker ◽  
Alanna Schepartz
Keyword(s):  
Structure And Function ◽  
Peptide Structure ◽  
Library Screen ◽  
And Function

scholarly journals Recent advances on gradient hydrogels in biomimetic cartilage tissue engineering

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 2158 ◽  
Author(s):  
Ivana Gadjanski
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Blood Vessels ◽  
Cartilage Tissue Engineering ◽  
Structure And Function ◽  
Cartilage Tissue ◽  
Cartilaginous Tissue ◽  
Zonal Structure ◽  
Promising Target ◽  
And Function

Articular cartilage (AC) is a seemingly simple tissue that has only one type of constituting cell and no blood vessels and nerves. In the early days of tissue engineering, cartilage appeared to be an easy and promising target for reconstruction and this was especially motivating because of widespread AC pathologies such as osteoarthritis and frequent sports-induced injuries. However, AC has proven to be anything but simple. Recreating the varying properties of its zonal structure is a challenge that has not yet been fully answered. This caused the shift in tissue engineering strategies toward bioinspired or biomimetic approaches that attempt to mimic and simulate as much as possible the structure and function of the native tissues. Hydrogels, particularly gradient hydrogels, have shown great potential as components of the biomimetic engineering of the cartilaginous tissue.

scholarly journals High-density lipoproteins for therapeutic delivery systems

2016 ◽  
Vol 4 (2) ◽  
pp. 188-197 ◽  
Author(s):  
R. Kannan Mutharasan ◽  
Linda Foit ◽  
C. Shad Thaxton
Keyword(s):  
Drug Delivery ◽  
Delivery Systems ◽  
Structure And Function ◽  
High Density ◽  
High Density Lipoproteins ◽  
Delivery Vehicle ◽  
Drug Delivery Vehicle ◽  
Therapeutic Delivery ◽  
And Function

High-density lipoproteins are a class of natural nanostructures with multiple desirable properties to model in a drug delivery vehicle. Here we review the structure and function of high-density lipoproteins, and their use as therapeutic delivery systems.

scholarly journals Controlling Structure and Function of Polymeric Drug Delivery Nanoparticles Using Microfluidics

2017 ◽  
Vol 14 (8) ◽  
pp. 2595-2606 ◽  
Author(s):  
Aman Bains ◽  
Yimeng Cao ◽  
Sundiata Kly ◽  
Jeremy E. Wulff ◽  
Matthew G. Moffitt
Keyword(s):  
Drug Delivery ◽  
Structure And Function ◽  
Polymeric Drug Delivery ◽  
Polymeric Drug ◽  
And Function

scholarly journals Micro- and nano-patterned conductive graphene–PEG hybrid scaffolds for cardiac tissue engineering

2017 ◽  
Vol 53 (53) ◽  
pp. 7412-7415 ◽  
Author(s):  
Alec S. T. Smith ◽  
Hyok Yoo ◽  
Hyunjung Yi ◽  
Eun Hyun Ahn ◽  
Justin H. Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Structure And Function ◽  
Cardiac Structure ◽  
Nano Scale ◽  
Cardiac Structure And Function ◽  
And Function ◽  
Hybrid Scaffolds

Topographic and graphene-functionalized culture substrates were fabricated to regulate cardiac structure and function through manipulation of micro- and nano-scale mechanical and electroconductive cues.

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