Near-infrared light and magnetic field dual-responsive porous silicon-based nanocarriers to overcome multidrug resistance in breast cancer cells with enhanced efficiency

2020 ◽  
Vol 8 (3) ◽  
pp. 546-557 ◽  
Author(s):  
Jiachen Li ◽  
Weiwei Zhang ◽  
Yan Gao ◽  
Haibei Tong ◽  
Zhenyu Chen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Magnetic Field ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Near Infrared ◽  
Drug Carriers ◽  
Infrared Light ◽  
Drug Resistant ◽  
Dual Responsive ◽  
Silicon Based

Magnetic and photothermal PSiNPs@(Fe3O4/Au) nanocomposites as anticancer drug carriers improved combined chemo-photothermal therapeutic efficacy of drug-resistant breast cancer cells.

scholarly journals Combination of Near-Infrared Photoimmunotherapy Using Trastuzumab and Small Protein Mimetic for HER2-Positive Breast Cancer

2021 ◽  
Vol 22 (22) ◽  
pp. 12213
Author(s):  
Haruka Yamaguchi ◽  
Jotaro On ◽  
Takao Morita ◽  
Takamasa Suzuki ◽  
Yasuo Okada ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Monoclonal Antibody ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Near Infrared ◽  
Infrared Light ◽  
Her2 Positive ◽  
Small Protein ◽  
Her2 Positive Breast Cancer ◽  
Positive Breast Cancer

Near-infrared photoimmunotherapy (NIR-PIT) is a promising cancer therapy based on a monoclonal antibody conjugated to a photosensitizer (IR700Dye) that is activated by near-infrared light irradiation. We previously reported on the use of NIR-PIT with a small protein mimetic, the Affibody molecule (6–7 kDa), instead of a monoclonal antibody. In this study, we investigated a combination of NIR-PIT for HER2-positive breast cancer cells (SK-BR3, MDA-MB361, and JIMT1) with HER2 Affibody-IR700Dye conjugate and trastuzumab-IR700Dye conjugate. HER2 Affibody and trastuzumab target different epitopes of the HER2 protein and do not compete. In vitro, the combination of NIR-PIT using both HER2 Affibody-IR700Dye conjugate and trastuzumab-IR700Dye conjugate induced necrotic cell death of HER2-positive breast cancer cells without damage to HER2-negative breast cancer cells (MCF7). It was more efficient than NIR-PIT using either the HER2 Affibody-IR700Dye conjugate alone or the trastuzumab-IR700Dye conjugate alone. Additionally, this combination of NIR-PIT was significantly effective against HER2 low-expressing cancer cells, trastuzumab-resistant cells (JIMT1), and brain metastatic cells of breast cancer (MDA-MB361). Furthermore, in vivo imaging exhibited the strong fluorescence intensity of both HER2 Affibody-IR700Dye conjugates and trastuzumab-Alexa488 conjugates in HER2-positive tumor, indicating that both HER2 Affibody and trastuzumab specifically bind to HER2-positive tumors without competing with each other. In conclusion, the combination of NIR-PIT using both HER2 Affibody and trastuzumab expands the targeting scope of NIR-PIT for HER2-positive breast cancer.

Sulfobetaine methacrylate-albumin-coated graphene oxide incorporating IR780 for enhanced breast cancer phototherapy

Nanomedicine ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. 453-464
Author(s):  
Bruna L Melo ◽  
Rita Lima-Sousa ◽  
Cátia G Alves ◽  
Paula Ferreira ◽  
André F Moreira ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Graphene Oxide ◽  
Bovine Serum Albumin ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Colloidal Stability ◽  
Near Infrared ◽  
Infrared Light ◽  
Near Infrared Light ◽  
Biologically Relevant

Aim: Enhance the colloidal stability and photothermal capacity of graphene oxide (GO) by functionalizing it with sulfobetaine methacrylate (SBMA)-grafted bovine serum albumin (BSA; i.e., SBMA- g-BSA) and by loading IR780, respectively. Materials & methods: SBMA- g-BSA coating and IR780 loading into GO was achieved through a simple sonication process. Results: SBMA- g-BSA-functionalized GO (SBMA-BSA/GO) presented an adequate size distribution and cytocompatibility. When in contact with biologically relevant media, the size of the SBMA-BSA/GO only increased by 8%. By loading IR780 into SBMA-BSA/GO, its photothermal capacity increased by twofold. The combination of near infrared light with SBMA-BSA/GO did not induce photocytotoxicity on breast cancer cells. In contrast, the interaction of IR780-loaded SBMA-BSA/GO with near infrared light caused the ablation of cancer cells. Conclusion: IR780-loaded SBMA-BSA/GO displayed an improved colloidal stability and phototherapeutic capacity.

scholarly journals Facile fabricating of rGO and Au/rGO nanocomposites using Brassica oleracea var. gongylodes biomass for non-invasive approach in cancer therapy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fatemeh Yousefimehr ◽  
Saeed Jafarirad ◽  
Roya Salehi ◽  
Mohammad Sadegh Zakerhamidi
Keyword(s):  
Breast Cancer ◽  
Green Synthesis ◽  
Cancer Cells ◽  
Brassica Oleracea ◽  
Photothermal Therapy ◽  
Breast Cancer Cells ◽  
Near Infrared ◽  
Therapeutic Effects ◽  
Invasive Approach ◽  
Au Nps

AbstractIn this study, we report a facile green-synthesis route for the fabrication of reduced graphene oxide (rGO) using biomass of Brassica oleracea var. gongylodes (B. oleracea). In addition, we have attempted to provide a green synthesis approach to prepare Gold nanoparticles (Au NPs) on the surface of rGO by using stem extract of B. oleracea. The synthesized Au/rGO nanocomposite was evaluated using UV–visible and FTIR spectroscopy, XRD, Raman, FE-SEM, EDX, AFM and DLS techniques. The obtained results demonstrated that the synthesized Au NPs on the surface of rGO was spherical with sizes ranging about 12–18 nm. The Au/rGO NC was, also, developed as photo-synthesizer system for the photothermal therapy (PTT) of MCF7 breast cancer cells. The near-infrared (NIR) photothermal properties of Au/rGO NCs was evaluated using a continuous laser at 808 nm with power densities of 1 W.cm−2. Their photothermal efficacy on MCF7 breast cancer cells after optimizing the proper concentration of the NCs were evaluated by MTT assay, Cell cycle and DAPI staining. In addition, the potential of the synthesized Au/rGO NCs on reactive oxygen species generating and antioxidant activity were assessed by DPPH. Au/rGO NCs possess high capacity to light-to-heat conversion for absorption in range NIR light, and it is able to therapeutic effects on MCF7 cells at a low concentration. The maximum amount of cell death is 40.12% which was observed in treatment groups that received a combination of Au/rGO NCs and laser irradiation. The results demonstrate that the nanomaterials synthesized by green approach lead to efficient destruction of cancer cell and might thus serve as an excellent theranostic agent in Photothermal therapy applications.

ABCB1/MDR1 contributes to the anticancer drug-resistant phenotype of IPH-926 human lobular breast cancer cells

2012 ◽  
Vol 315 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Till Krech ◽  
Elisa Scheuerer ◽  
Robert Geffers ◽  
Hans Kreipe ◽  
Ulrich Lehmann ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Anticancer Drug ◽  
Breast Cancer Cells ◽  
Drug Resistant ◽  
Lobular Breast Cancer ◽  
Resistant Phenotype ◽  
Drug Resistant Phenotype

scholarly journals MicroRNA‑16 sensitizes drug‑resistant breast cancer cells to Adriamycin by targeting Wip1 and Bcl‑2

2019 ◽  
Author(s):  
Xitao Gao ◽  
Mei Wang ◽  
Yanyan Zhang ◽  
Zhi Xu ◽  
Jiaji Ding ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Drug Resistant
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