A nanowire-integrated thermoresponsive microfluidic platform for on-demand enrichment and colorimetric detection of pathogenic bacteria

2019 ◽  
Vol 7 (46) ◽  
pp. 7301-7305
Author(s):  
Xuancheng Du ◽  
Chun Wu ◽  
Weijing Wang ◽  
Lin Qiu ◽  
Pengju Jiang ◽  
...  

A nanowire-integrated thermoresponsive microfluidic platform has been presented for on-demand enrichment and colorimetric detection of pathogenic bacteria.

RSC Advances ◽  
2018 ◽  
Vol 8 (48) ◽  
pp. 27091-27100 ◽  
Author(s):  
Abbas Motalebizadeh ◽  
Hasan Bagheri ◽  
Sasan Asiaei ◽  
Nasim Fekrat ◽  
Abbas Afkhami

A smartphone-based microfluidic platform was developed for point-of-care (POC) detection using surface plasmon resonance (SPR) of gold nanoparticles (GNPs).


Lab on a Chip ◽  
2018 ◽  
Vol 18 (4) ◽  
pp. 610-619 ◽  
Author(s):  
Lu Zhang ◽  
Fei Tian ◽  
Chao Liu ◽  
Qiang Feng ◽  
Tingxuan Ma ◽  
...  

A hand-powered centrifugal microfluidic platform enables simultaneous detection of pathogenic bacteria within a small disc in an electricity-free manner.


The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7320-7329
Author(s):  
Muhammad Asif ◽  
Fazli Rabbi Awan ◽  
Qaiser Mahmood Khan ◽  
Bongkot Ngamsom ◽  
Nicole Pamme

We investigate paper microfluidic devices for detection of pathogenic bacteria and their sensitivity towards β-lactamase and Extended Spectrum Beta Lactamases (ESBLs) in milk samples to enable appropriate prescription of antibiotics for mastitis.


Lab on a Chip ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 493-502 ◽  
Author(s):  
Hesam Babahosseini ◽  
Tom Misteli ◽  
Don L. DeVoe

A multifunctional microfluidic platform combining on-demand aqueous-phase droplet generation, multi-droplet storage, and controlled merging of droplets selected from a storage library in a single integrated microfluidic device is described.


2015 ◽  
Vol 883 ◽  
pp. 61-66 ◽  
Author(s):  
Donghoon Kwon ◽  
Sanghee Lee ◽  
Myung Mo Ahn ◽  
In Seok Kang ◽  
Ki-Hwan Park ◽  
...  

Author(s):  
Nhat-Khuong Nguyen ◽  
Pradip Singha ◽  
Hongjie An ◽  
Hoang-Phuong Phan ◽  
Nam-Trung Nguyen ◽  
...  

Liquid marble is a promising microfluidic platform for microreactor applications. However, the lack of contactless and on-demand mixing strategies significantly hinders its potential. This paper reports the use of electrostatic...


2016 ◽  
Author(s):  
Kristina Doxsee ◽  
Ryan Berthelot ◽  
Suresh Neethirajan

Electrotaxis or galvanotaxis refers to the migration pattern of cells induced in response to electrical potential. Although it has been extensively studied in mammalian cells, electrotaxis has not been explored in detail in bacterial cells; information regarding the impact of current on pathogenic bacteria is severely lacking. Therefore, we designed a series of single and multi-cue experiments to assess the impact of varying currents on bacterial motility dynamics in pathogenic multi-drug resistant (MDR) strains of Pseudomonas aeruginosa and Escherichia coli using a microfluidic platform. Motility plays key roles in bacterial migration and the colonization of surfaces during the formation of biofilms, which are inherently recalcitrant to removal and resistant to traditional disinfection strategies (e.g. antibiotics). Use of the microfluidic platform allows for exposure to current, which can be supplied at a range that is biocidal to bacteria, yet physiologically safe in humans (single cue). This system also allows for multi-cue experiments where acetic acid, a relatively safe compound with anti-fouling/antimicrobial properties, can be combined with current to enhance disinfection. These strategies may offer substantial therapeutic benefits, specifically for the treatment of biofilm infections, such as those found in the wound environment. Furthermore, microfluidic systems have been successfully used to model the unique microfluidic dynamics present in the wound environment, suggesting that these investigations could be extended to more complex biological systems. Our results showed that the application of current in combination with acetic acid has profound inhibitory effects on MDR strains of P. aeruginosa and E. coli, even with brief applications. Specifically, E. coli motility dynamics and cell survival were significantly impaired starting at a concentration of 125 μA DC and 0.31% acetic acid, while P. aeruginosa was impaired at 70 μA and 0.31% acetic acid. As these strains are relevant wound pathogens, it is likely that this strategy would be effective against similar strains in vivo and could represent a new approach to hasten wound healing.


Author(s):  
Sarah L. Castro ◽  
William T. Wallace ◽  
Kathie Thomas-Keprta ◽  
Lindsay Keller ◽  
Mark Ott

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