A superparamagnetic Fe3O4–TiO2 composite coating on titanium by micro-arc oxidation for percutaneous implants

2019 ◽  
Vol 7 (34) ◽  
pp. 5265-5276 ◽  
Author(s):  
Kai Li ◽  
Shiyu Liu ◽  
Yang Xue ◽  
Lan Zhang ◽  
Yong Han
Keyword(s):  
Magnetic Field ◽  
Composite Coating ◽  
Micro Arc Oxidation ◽  
Percutaneous Implants

The micro-magnetic field induced by the Fe3O4 nanoparticles in TiO2 can efficiently enhance the fibroblast response, reduce bacterial reproduction in vitro, and improve skin integration in vivo.

scholarly journals Magnetic Nanodiscs—A New Promising Tool for Microsurgery of Malignant Neoplasms

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1459
Author(s):  
Tatiana N. Zamay ◽  
Vladimir S. Prokopenko ◽  
Sergey S. Zamay ◽  
Kirill A. Lukyanenko ◽  
Olga S. Kolovskaya ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Remote Control ◽  
Tumor Cells ◽  
Biological Effects ◽  
Malignant Neoplasms ◽  
Rotating Magnetic Fields ◽  
Magnetic Structures ◽  
Promising Tool

Magnetomechanical therapy is one of the most perspective directions in tumor microsurgery. According to the analysis of recent publications, it can be concluded that a nanoscalpel could become an instrument sufficient for cancer microsurgery. It should possess the following properties: (1) nano- or microsized; (2) affinity and specificity to the targets on tumor cells; (3) remote control. This nano- or microscalpel should include at least two components: (1) a physical nanostructure (particle, disc, plates) with the ability to transform the magnetic moment to mechanical torque; (2) a ligand—a molecule (antibody, aptamer, etc.) allowing the scalpel precisely target tumor cells. Literature analysis revealed that the most suitable nanoscalpel structures are anisotropic, magnetic micro- or nanodiscs with high-saturation magnetization and the absence of remanence, facilitating scalpel remote control via the magnetic field. Additionally, anisotropy enhances the transmigration of the discs to the tumor. To date, four types of magnetic microdiscs have been used for tumor destruction: synthetic antiferromagnetic P-SAF (perpendicular) and SAF (in-plane), vortex Py, and three-layer non-magnetic–ferromagnet–non-magnetic systems with flat quasi-dipole magnetic structures. In the current review, we discuss the biological effects of magnetic discs, the mechanisms of action, and the toxicity in alternating or rotating magnetic fields in vitro and in vivo. Based on the experimental data presented in the literature, we conclude that the targeted and remotely controlled magnetic field nanoscalpel is an effective and safe instrument for cancer therapy or theranostics.

Development and Test of an Extendable Endoprosthesis for Bone Reconstruction in the Leg

1994 ◽  
Vol 17 (3) ◽  
pp. 155-162 ◽  
Author(s):  
G.J. Verkerke ◽  
H. Schraffordt Koops ◽  
R.P.H. Veth ◽  
H.J. Grootenboer ◽  
L.J. De Boer ◽  
...  
Keyword(s):  
Magnetic Field ◽  
External Magnetic Field ◽  
Growth Plate ◽  
Animal Experiments ◽  
Bone Tumour ◽  
In Vitro Tests ◽  
Bone Reconstruction ◽  
Malignant Bone Tumour

A malignant bone tumour may develop in the femur of a child. In the majority of cases it will be necessary to resect the bone involved, growth plate and adjacent tissues. A modular endoprosthetic system has been developed which can be extended non-invasively to bridge the defect resulting from such a resection. Elongation is achieved by using an external magnetic field. In vitro tests with a prototype showed that the lengthening element met all requirements. Six animal experiments showed that the lengthening element also functioned in vivo.

Magic-TT (Magnetism-induced cell target transplantation) Enhanced the CD45+ Cells Target Migration, in Situ Proliferation and Promotion of Hematopoietic Recovery after Transplantation

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 5404-5404
Author(s):  
Qianli Jiang ◽  
Hao Huang ◽  
Yongjun Zhou ◽  
Qiuxia Zhang ◽  
Sun Xiaowei ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Bone Marrow ◽  
Stem Cell ◽  
Cell Transplantation ◽  
In Vitro Study ◽  
Confocal Microscope ◽  
Hematopoietic Recovery ◽  
Donor Cells

Abstract Background: In our previous work (56th ASH poster, No.2416), we developed a novel cell transplantation system named MagIC-TT. The purpose of this study is to explore whether the MagIC-TT can promote hematopoietic recovery in the mice experiment and illustrate it¡¯s mechanism both in vivo and in vitro. Methods: 1) In vivo study: With regard to auto-transplantation, the C57BL/6 CD45-GFP cells were sorted and magnetized from the bone marrow of C57BL/6-Tg(CAG-EGFP) mice. Forty C57BL/6 female mice (2 groups, twenty mice each group) were transplanted into the femur cavity with or without magnetic field (M or W group), after 7.5Gy irradiation. Following transplantation, the survival of mice, hematopoiesis as well as GFP+ cells in different tissues, such as peripheral blood, bone marrow, liver, spleen, thymus and lung etc. were observed. Femurs of recipients were decalcified with our own derived semi-solid decalcification (SSD) technique to illustrate the distribution, proliferation of donor cells and the relationship between recipients and donor cells. Allo-transplantation: The C57BL/6 CD45-GFP cells were injected into the femur cavity of FVB mRFP transgenic mice (sponsored by Prof. XH Wu, Fudan University, Shanghai, China) after 7.5Gy irradiation. GVHD was observed in addition to what was done in auto-transplantation. 2) In vitro study: Magnetized CD45-GFP cells and non-magnetized BMSC-RFPs were cultured respectively or co-cultured with or without magnetic field (M or W group). The magnetic field was added to the top or the bottom of cell culture dish. Cell morphology, cell proliferation, cell viability, as well as cell migration, transwell migration and matrigel migration assays induced by magnetism were studied. The interaction of CD45-GFP cells and BMSC-RFPs was observed by confocal microscope, electronic microscope, immunohistochemical staining, western blot, real-time PCR and deep sequencing. Results: 1) In vivo study: During the first few hours after transplantation, lots of magnetized CD45-GFP cells resided within the femur and knee joints in M group while few in W group. Many GFP cells migrated into the lung soon after transplantation in the W group (P =0.046), followed by other organs such as kidney and skin (Fig.1). FACS showed that more GFP+ cells resided within the target femurs than the controls (Table.1). With SSD, frozen sections, confocal microscope and Lightsheet Z.1 Microimage (Carl Zeiss); transplanted GFP+ cells and their micro-environment were all well demonstrated (Fig.1). On removal of magnetic field, CD45-GFP cells were observed to migrate into the spleen, kidney, gut and other organs, showing the slow release of target transplanted cells from femur. GVHD on skin and lung etc. were observed in C57BL/6 to FVB allogenic transplanted mice (Fig. 1). The hematopoietic recovery in M group occurs much earlier than the controls, especially for the platelets, 10.67d ¡À 1.53d vs 14.75d ¡À 2.06d (M vs W group, P =0.035). 2) In vitro study: With the help of MagIC-TT, CD45-GFP cells can migrate through the matrigel and transwell membranes much more efficiently. The magnetized CD45-GFP cells advance toward the inner roof of petri dish in the culture medium, and attach to BMSC-RFP growing on the inner roof of dish and proliferate in the niche composed by BMSC-RFP under the effect of magnetic field (Fig.2). Conclusion: MagIC-TT could enhance CD45+ cells target migration, improve stem cell homing and proliferation efficiency, as well as promotion hematopoietic recovery in vivo. This study would shed light on current Hematological Stem Cell Transplantation (HSCT) and other cell therapies. Table 1. The FACS results of femurs of CD45-GFP cells injected into C57 mice, at 0.5h, 24h and 72h respectively. group 0.5h£¨%£© p 24h£¨%£© p 72h£¨%£© p *LC **RT *LC **RT *LC **RT BMM 0.017¡À0.006 0.497¡À0.151 0.040 0.080¡À0.026 1.573¡À0.508 0.030 0.190¡À0.139 1.960¡À0.809 0.049 BMW 0.017¡À0.012 0.050¡À0.017 0.184 0.013¡À0.006 0.027¡À0.015 0.184 0.023¡À0.015 0.320¡À0.434 0.368 P 1.000 0.007 0.013 0.006 0.108 0.036 *LC: Control femur without magnetic field (W group); **RT: Treated femur with magnetic field (M group). Disclosures No relevant conflicts of interest to declare.

In-vitro and in-vivo study of periodontal ligament cryopreserved with a magnetic field

2011 ◽  
Vol 140 (6) ◽  
pp. 799-805 ◽  
Author(s):  
Hiroko Kamada ◽  
Masato Kaku ◽  
Toshitsugu Kawata ◽  
Hiroyuki Koseki ◽  
Sara Abedini ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Periodontal Ligament ◽  
In Vivo Study

Abstract No. 122: Alternating magnetic field thermotherapy of liver tumors: preliminary in vitro and in vivo study

2012 ◽  
Vol 23 (3) ◽  
pp. S51-S52
Author(s):  
E. Liapi ◽  
S. Mirpour ◽  
M. Wabler ◽  
H. Zhou ◽  
Y. Zhang ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Liver Tumors ◽  
Alternating Magnetic Field ◽  
In Vivo Study

scholarly journals Hydroxyapatite-Coated Titanium by Micro-Arc Oxidation and Steam–Hydrothermal Treatment Promotes Osseointegration

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiaojun Wang ◽  
Lina Mei ◽  
Xuesheng Jiang ◽  
Mingchao Jin ◽  
Yan Xu ◽  
...  
Keyword(s):  
Hydrothermal Treatment ◽  
Cell Attachment ◽  
Vascular Endothelial ◽  
Matrix Mineralization ◽  
X Ray ◽  
Micro Arc Oxidation ◽  
Surface Physicochemical Properties ◽  
Endothelial Growth Factor

Titanium (Ti)-based alloys are widely used in tissue regeneration with advantages of improved biocompatibility, high mechanical strength, corrosion resistance, and cell attachment. To obtain bioactive bone–implant interfaces with enhanced osteogenic capacity, various methods have been developed to modify the surface physicochemical properties of bio-inert Ti and Ti alloys. Nano-structured hydroxyapatite (HA) formed by micro-arc oxidation (MAO) is a synthetic material, which could facilitate osteoconductivity, osteoinductivity, and angiogenesis on the Ti surface. In this paper, we applied MAO and steam–hydrothermal treatment (SHT) to produce HA-coated Ti, hereafter called Ti–M–H. The surface morphology of Ti–M–H1 was observed by scanning electron microscopy (SEM), and the element composition and the roughness of Ti–M–H1 were analyzed by energy-dispersive X-ray analysis, an X-ray diffractometer (XRD), and Bruker stylus profiler, demonstrating the deposition of nano-HA particles on Ti surfaces that were composed of Ca, P, Ti, and O. Then, the role of Ti–M–H in osteogenesis and angiogenesis in vitro was evaluated. The data illustrated that Ti–M–H1 showed a good compatibility with osteoblasts (OBs), which promoted adhesion, spreading, and proliferation. Additionally, the secretion of ALP, Col-1, and extracellular matrix mineralization was increased by OBs treated with Ti–M–H1. Ti–M–H1 could stimulate endothelial cells to secrete vascular endothelial growth factor and promote the formation of capillary-like networks. Next, it was revealed that Ti–M–H1 also suppressed inflammation by activating macrophages, while releasing multiple active factors to mediate osteogenesis and angiogenesis. Finally, in vivo results uncovered that Ti–M–H1 facilitated a higher bone-to-implant interface and was more attractive for the dendrites, which promoted osseointegration. In summary, MAO and SHT-treated Ti–M–H1 not only promotes in vitro osteogenesis and angiogenesis but also induces M2 macrophages to regulate the immune environment, which enhances the crosstalk between osteogenesis and angiogenesis and ultimately accelerates the process of osseointegration in vivo.

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