In situ biosynthesized gold nanoclusters inhibiting cancer development via the PI3K–AKT signaling pathway

2019 ◽  
Vol 7 (35) ◽  
pp. 5336-5344 ◽  
Author(s):  
Maonan Wang ◽  
Zeqian Yu ◽  
Huan Feng ◽  
Jianling Wang ◽  
Lishan Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Signaling Pathway ◽  
Gold Nanoclusters ◽  
Akt Signaling ◽  
Cancer Development ◽  
Akt Signaling Pathway

Nanomaterials have made great breakthroughs in drug delivery.

scholarly journals CEMIP promotes ovarian cancer development and progression via the PI3K/AKT signaling pathway

2019 ◽  
Vol 114 ◽  
pp. 108787 ◽  
Author(s):  
Fan Shen ◽  
Zhi-hong Zong ◽  
Yao Liu ◽  
Shuo Chen ◽  
Xiu-jie Sheng ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Cancer Development ◽  
Akt Signaling Pathway

scholarly journals CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Tao Jiang ◽  
Hongyu Wang ◽  
Lianyu Liu ◽  
Hu Song ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
In Situ Hybridization ◽  
Signaling Pathway ◽  
Clinical Significance ◽  
Akt Signaling ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Akt Signaling Pathway ◽  
Aberrant Expression

Abstract Background Accumulating studies have revealed that aberrant expression of circular RNAs (circRNAs) is widely involved in the tumorigenesis and progression of malignant cancers, including colorectal cancer (CRC). Nevertheless, the clinical significance, levels, features, biological function, and molecular mechanisms of novel circRNAs in CRC remain largely unexplored. Methods CRC-related circRNAs were identified through bioinformatics analysis and verified in clinical specimens by qRT–PCR and in situ hybridization (ISH). Then, in vitro and in vivo experiments were performed to determine the clinical significance of, functional roles of, and clinical characteristics associated with circIL4R in CRC specimens and cells. Mechanistically, RNA pull-down, fluorescence in situ hybridization (FISH), luciferase reporter, and ubiquitination assays were performed to confirm the underlying mechanism of circIL4R. Results CircIL4R was upregulated in CRC cell lines and in sera and tissues from CRC patients and was positively correlated with advanced clinicopathological features and poor prognosis. Functional experiments demonstrated that circIL4R promotes CRC cell proliferation, migration, and invasion via the PI3K/AKT signaling pathway. Mechanistically, circIL4R was regulated by TFAP2C and competitively interacted with miR-761 to enhance the expression of TRIM29, thereby targeting PHLPP1 for ubiquitin-mediated degradation to activate the PI3K/AKT signaling pathway and consequently facilitate CRC progression. Conclusions Our findings demonstrate that upregulation of circIL4R plays an oncogenic role in CRC progression and may serve as a promising diagnostic and prognostic biomarker for CRC detection and as a potential therapeutic target for CRC treatment.

scholarly journals Circular RNA CircCOL5A1 Sponges the MiR-7-5p/Epac1 Axis to Promote the Progression of Keloids Through Regulating PI3K/Akt Signaling Pathway

2021 ◽  
Vol 9 ◽  
Author(s):  
Wenchang Lv ◽  
Shengxuan Liu ◽  
Qi Zhang ◽  
Weijie Hu ◽  
Yiping Wu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Signaling Pathway ◽  
Therapeutic Potential ◽  
Akt Signaling ◽  
Luciferase Reporter ◽  
Akt Signaling Pathway ◽  
Luciferase Reporter Gene ◽  
Migration Flow ◽  
Gene Assay

Keloids, as a result of abnormal wound healing in susceptible individuals, are characterized by the hyper-proliferation of fibroblasts and exaggerated deposition of extracellular matrix. Current surgical and therapeutic modalities provide limited satisfactory results. Growing evidence has highlighted the roles of circRNAs in acting as miRNA sponges. However, up to date, the regulatory mechanism of circRNAs in the pathological process of keloids has rarely been reported. In this study, cell proliferation, cell migration, flow cytometry, western blotting, fluorescence in situ hybridization, dual-luciferase activity, and immunohistochemistry assays were applied to explore the roles and mechanisms of the circCOL5A1/miR-7-5p/Epac1 axis in the keloid. The therapeutic potential of circCOL5A1 was investigated by establishing keloid implantation models. The RT-qPCR result revealed that circCOL5A1 expression was obviously higher in keloid tissues and keloid fibroblasts. Subsequent cellular experiments demonstrated that circCOL5A1 knockdown repressed the proliferation, migration, extracellular matrix (ECM) deposition, whereas promoted cell apoptosis, through the PI3K/Akt signaling pathway. Furthermore, RNA-fluorescence in situ hybridization (RNA-FISH) illustrated that both circCOL5A1 and miR-7-5p were located in the cytoplasm. The luciferase reporter gene assay confirmed that exact binding sites were present between circCOL5A1 and miR-7-5p, as well as between miR-7-5p and Epac1. Collectively, the present study revealed that circCOL5A1 functioned as competing endogenous RNA (ceRNA) by adsorbing miR-7-5p to release Epac1, which contributed to pathological hyperplasia of keloids through activating the PI3K/Akt signaling pathway. Our data indicated that circCOL5A1 might serve as a novel promising therapeutic target and represent a new avenue to understand underlying pathogenesis for keloids.

scholarly journals HMGB1 Promotes Prostate Cancer Development and Metastasis by Interacting with Brahma-Related Gene 1 and Activating the Akt Signaling Pathway

Theranostics ◽  
2019 ◽  
Vol 9 (18) ◽  
pp. 5166-5182 ◽  
Author(s):  
Dao‑Jun Lv ◽  
Xian‑Lu Song ◽  
Bin Huang ◽  
Yu-Zhong Yu ◽  
Fang‑Peng Shu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Cancer Development ◽  
Related Gene ◽  
Akt Signaling Pathway ◽  
Prostate Cancer Development

scholarly journals EIF3D promotes gallbladder cancer development by stabilizing GRK2 kinase and activating PI3K-AKT signaling pathway

2017 ◽  
Vol 8 (6) ◽  
pp. e2868-e2868 ◽  
Author(s):  
Fei Zhang ◽  
Shanshan Xiang ◽  
Yang Cao ◽  
Maolan Li ◽  
Qiang Ma ◽  
...  
Keyword(s):  
Gallbladder Cancer ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Cancer Development ◽  
Akt Signaling Pathway
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