A surface-engineered NIR light-responsive actuator for controllable modulation of collective cell migration

2019 ◽  
Vol 7 (36) ◽  
pp. 5528-5534 ◽  
Author(s):  
Jiayu Liu ◽  
Jinhui Shang ◽  
Yancao Chen ◽  
Yueyue Tian ◽  
Qian Yang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Cell Migration ◽  
Live Cells ◽  
Collective Cell Migration ◽  
Nir Light ◽  
Mechanical Signal

A surface-engineered NIR light-responsive actuator has been presented for manipulating collective cell migration by activating mechanical signal transduction in live cells.

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

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