Selective recognition of CdTe QDs and strand displacement signal amplification-assisted label-free and homogeneous fluorescence assay of nucleic acid and protein

2019 ◽  
Vol 7 (31) ◽  
pp. 4778-4783 ◽  
Author(s):  
Pingyue Hu ◽  
Xiu Wang ◽  
Long Wei ◽  
Rui Dai ◽  
Xin Yuan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Fluorescence Assay ◽  
Selective Recognition ◽  
Label Free ◽  
Strand Displacement ◽  
Cdte Qds ◽  
Displacement Signal

Selective recognition of CdTe QDs and strand displacement signal amplification-assisted label-free and homogeneous fluorescence assay of nucleic acid and protein.

A label-free signal amplification assay for DNA detection based on exonuclease III and nucleic acid dye SYBR Green I

Talanta ◽  
2013 ◽  
Vol 114 ◽  
pp. 49-53 ◽  
Author(s):  
Aihua Zheng ◽  
Ming Luo ◽  
Dongshan Xiang ◽  
Xia Xiang ◽  
Xinghu Ji ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Dna Detection ◽  
Sybr Green ◽  
Sybr Green I ◽  
Label Free ◽  
Acid Dye ◽  
Exonuclease Iii ◽  
Amplification Assay

A sensitive SERS detection of miRNA using a label-free multifunctional probe

2015 ◽  
Vol 51 (94) ◽  
pp. 16836-16839 ◽  
Author(s):  
Hao Zhang ◽  
Yu Liu ◽  
Jian Gao ◽  
Junhui Zhen
Keyword(s):  
Nucleic Acid ◽  
Raman Scattering ◽  
Detection Method ◽  
Surface Enhanced Raman Scattering ◽  
Label Free ◽  
Strand Displacement ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Enhanced Raman Scattering

A novel surface enhanced Raman scattering (SERS) detection method is fabricated for miRNA based on a smart multifunctional probe for dual cyclical nucleic acid strand-displacement polymerization (CNDP).

scholarly journals A kinetically controlled platform for ligand-oligonucleotide transduction

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Qiu-Long Zhang ◽  
Liang-Liang Wang ◽  
Yan Liu ◽  
Jiao Lin ◽  
Liang Xu
Keyword(s):  
Nucleic Acid ◽  
Small Molecules ◽  
Signal Amplification ◽  
Kinetic Control ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Critical Pathway ◽  
Kinetically Controlled ◽  
Binding Sequence ◽  
Strand Displacement Reaction

AbstractLigand-oligonucleotide transduction provides the critical pathway to integrate non-nucleic acid molecules into nucleic acid circuits and nanomachines for a variety of strand-displacement related applications. Herein, a general platform is constructed to convert the signals of ligands into desired oligonucleotides through a precise kinetic control. In this design, the ligand-aptamer binding sequence with an engineered duplex stem is introduced between the toehold and displacement domains of the invading strand to regulate the strand-displacement reaction. Employing this platform, we achieve efficient transduction of both small molecules and proteins orthogonally, and more importantly, establish logical and cascading operations between different ligands for versatile transduction. Besides, this platform is capable of being directly coupled with the signal amplification systems to further enhance the transduction performance. This kinetically controlled platform presents unique features with designing simplicity and flexibility, expandable complexity and system compatibility, which may pave a broad road towards nucleic acid-based developments of sophisticated transduction networks.

An ATP-fueled nucleic acid signal amplification strategy for highly sensitive microRNA detection

2018 ◽  
Vol 54 (77) ◽  
pp. 10897-10900 ◽  
Author(s):  
Zhi-Bin Wen ◽  
Wen-Bin Liang ◽  
Ying Zhuo ◽  
Cheng-Yi Xiong ◽  
Ying-Ning Zheng ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Nucleic Acid ◽  
Signal Amplification ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Strand Displacement ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21.

Detection of lead(II) ions with a DNAzyme and isothermal strand displacement signal amplification

2014 ◽  
Vol 53 ◽  
pp. 245-249 ◽  
Author(s):  
Wenying Li ◽  
Yue Yang ◽  
Jian Chen ◽  
Qingfeng Zhang ◽  
Yan Wang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Strand Displacement ◽  
Displacement Signal
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