BODIPY-based hydrazine as a fluorescent probe for sensitive and selective detection of nitric oxide: a new strategy

2019 ◽  
Vol 7 (24) ◽  
pp. 3792-3795 ◽  
Author(s):  
Ying-Long Fu ◽  
Hao Li ◽  
Xiu-Zhi Wei ◽  
Qin-Hua Song
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
Selective Detection ◽  
Reactive Site ◽  
New Strategy

A novel fluorescent probe 8-HB was developed with a BODIPY as a fluorophore and 8-substituted hydrazine as a reactive site for sensitive and selective detection of nitric oxide (NO), generating major fluorescent dehydrazinated BODIPY and minor non-fluorescent azide BODIPY.

Highly Water-Soluble BODIPY-Based Fluorescent Probe for Sensitive and Selective Detection of Nitric Oxide in Living Cells

2013 ◽  
Vol 5 (10) ◽  
pp. 4107-4112 ◽  
Author(s):  
Giri K. Vegesna ◽  
Srinivas R. Sripathi ◽  
Jingtuo Zhang ◽  
Shilei Zhu ◽  
Weilue He ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Water Soluble ◽  
Selective Detection

A rhodamine-deoxylactam based fluorescent probe for fast and selective detection of nitric oxide in living cells

Talanta ◽  
2019 ◽  
Vol 197 ◽  
pp. 436-443 ◽  
Author(s):  
Wen-Li Jiang ◽  
Yongfei Li ◽  
Hong-Wen Liu ◽  
Dong-Ye Zhou ◽  
Juan Ou-Yang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Selective Detection

AIE-active Schiff base compounds as fluorescent probe for the highly sensitive and selective detection of Al3+ ions

2021 ◽  
Vol 233 ◽  
pp. 117911
Author(s):  
Minmin Wang ◽  
Linxia Lu ◽  
Wenwu Song ◽  
Xunyue Wang ◽  
Tongming Sun ◽  
...  
Keyword(s):  
Schiff Base ◽  
Fluorescent Probe ◽  
Selective Detection ◽  
Highly Sensitive

scholarly journals The in vitro manipulation of carbohydrate metabolism: a new strategy for deciphering the cellular defence mechanisms against nitric oxide attack

1999 ◽  
Vol 344 (3) ◽  
pp. 643 ◽  
Author(s):  
Claire LE GOFFE ◽  
Geneviève VALLETTE ◽  
Anne JARRY ◽  
Chantal BOU-HANNA ◽  
Christian L. LABOISSE
Keyword(s):  
Nitric Oxide ◽  
Carbohydrate Metabolism ◽  
Defence Mechanisms ◽  
New Strategy ◽  
Cellular Defence

scholarly journals Hydrogen Sulfide Increases Nitric Oxide Production and Subsequent S-Nitrosylation in Endothelial Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ping-Ho Chen ◽  
Yaw-Syan Fu ◽  
Yun-Ming Wang ◽  
Kun-Han Yang ◽  
Danny Ling Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Hydrogen Sulfide ◽  
Protein Kinase ◽  
Fluorescent Probe ◽  
Acid Methyl Ester ◽  
High Specificity ◽  
Protein S ◽  
No Production ◽  
No Donor

Hydrogen sulfide (H2S) and nitric oxide (NO), two endogenous gaseous molecules in endothelial cells, got increased attention with respect to their protective roles in the cardiovascular system. However, the details of the signaling pathways between H2S and NO in endothelia cells remain unclear. In this study, a treatment with NaHS profoundly increased the expression and the activity of endothelial nitric oxide synthase. Elevated gaseous NO levels were observed by a novel and specific fluorescent probe, 5-amino-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid methyl ester (FA-OMe), and quantified by flow cytometry. Further study indicated an increase of upstream regulator for eNOS activation, AMP-activated protein kinase (AMPK), and protein kinase B (Akt). By using a biotin switch, the level of NO-mediated protein S-nitrosylation was also enhanced. However, with the addition of the NO donor, NOC-18, the expressions of cystathionine-γ-lyase, cystathionine-β-synthase, and 3-mercaptopyruvate sulfurtransferase were not changed. The level of H2S was also monitored by a new designed fluorescent probe, 4-nitro-7-thiocyanatobenz-2-oxa-1,3-diazole (NBD-SCN) with high specificity. Therefore, NO did not reciprocally increase the expression of H2S-generating enzymes and the H2S level. The present study provides an integrated insight of cellular responses to H2S and NO from protein expression to gaseous molecule generation, which indicates the upstream role of H2S in modulating NO production and protein S-nitrosylation.

Sign in / Sign up

Export Citation Format

Share Document