scholarly journals Interfacial rheology and direct imaging reveal domain-templated network formation in phospholipid monolayers penetrated by fibrinogen

Soft Matter ◽  
2019 ◽  
Vol 15 (44) ◽  
pp. 9076-9084 ◽  
Author(s):  
Ian Williams ◽  
Joseph A. Zasadzinski ◽  
Todd M. Squires

The shear response of a phospholipid monolayer penetrated by fibrinogen is determined by the formation of a domain-templated protein network.

2017 ◽  
Vol 94 (3) ◽  
pp. 485-490 ◽  
Author(s):  
Lomme J. Deleu ◽  
Edith Wilderjans ◽  
Ingrid Van Haesendonck ◽  
Kristof Brijs ◽  
Jan A. Delcour

2020 ◽  
Vol 66 ◽  
pp. 102484 ◽  
Author(s):  
Ine Rombouts ◽  
Arno G.B. Wouters ◽  
Marlies A. Lambrecht ◽  
Lien Uten ◽  
Wouter Van Den Bosch ◽  
...  

2018 ◽  
Vol 15 (141) ◽  
pp. 20170895 ◽  
Author(s):  
Ian Williams ◽  
Todd M. Squires

All mammals depend on lung surfactant (LS) to reduce surface tension at the alveolar interface and facilitate respiration. The inactivation of LS in acute respiratory distress syndrome (ARDS) is generally accompanied by elevated levels of fibrinogen and other blood plasma proteins in the alveolar space. Motivated by the mechanical role fibrinogen may play in LS inactivation, we measure the interfacial rheology of mixed monolayers of fibrinogen and dipalmitoylphosphatidylcholine (DPPC), the main constituent of LS, and compare these to the single species monolayers. We find DPPC to be ineffective at displacing preadsorbed fibrinogen, which gives the resulting mixed monolayer a strongly elastic shear response. By contrast, how effectively a pre-existing DPPC monolayer prevents fibrinogen adsorption depends upon its surface pressure. At low DPPC surface pressures, fibrinogen penetrates DPPC monolayers, imparting a mixed viscoelastic shear response. At higher initial DPPC surface pressures, this response becomes increasingly viscous-dominated, and the monolayer retains a more fluid, DPPC-like character. Fluorescence microscopy reveals that the mixed monolayers exhibit qualitatively different morphologies. Fibrinogen has a strong, albeit preparation-dependent, mechanical effect on phospholipid monolayers, which may contribute to LS inactivation and disorders such as ARDS.


2016 ◽  
Vol 61 ◽  
pp. 409-414 ◽  
Author(s):  
Lomme J. Deleu ◽  
Edith Wilderjans ◽  
Ingrid Van Haesendonck ◽  
Kristof Brijs ◽  
Jan A. Delcour

2013 ◽  
Vol 59 (5) ◽  
pp. 519-522
Author(s):  
N.A. Kontarov ◽  
I.V. Pogarskaya ◽  
N.V. Balaev ◽  
N.V. Yuminova

The surface-active properties of 1-boraadamantane have been studied using model phospholipid monolayers. Results suggest that the increase in 1-boraadamantane concentrations from 10-7 to 10-6 M is accompanied by the increase of the area per phospholipid molecule. This decreases to frequency of lateral diffusion of phospholipids molecules, the potential difference and the angle of the phospholipid monolayer arrangement. These phenomena may lead to impossibility of interaction between the virus and cell membranes.


2017 ◽  
Vol 63 ◽  
pp. 226-232 ◽  
Author(s):  
Lomme J. Deleu ◽  
Sara Melis ◽  
Edith Wilderjans ◽  
Ingrid Van Haesendonck ◽  
Kristof Brijs ◽  
...  

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