scholarly journals Colloidal crystals of compliant microgel beads to study cell migration and mechanosensitivity in 3D

Soft Matter ◽  
2019 ◽  
Vol 15 (47) ◽  
pp. 9776-9787 ◽  
Author(s):  
Katrin Wagner ◽  
Salvatore Girardo ◽  
Ruchi Goswami ◽  
Gonzalo Rosso ◽  
Elke Ulbricht ◽  
...  
Keyword(s):  
Cell Migration ◽  
Colloidal Crystal ◽  
Colloidal Crystals ◽  
Variable Stiffness ◽  
Ecm Proteins

We present a novel 3D colloidal crystal made of polyacrylamide microgel beads with variable stiffness. The beads can be functionalized with ECM proteins and used to study mechanosensing responses of cells to varying stiffness in a 3D context.

scholarly journals Synthesis of magnetic mesoporous titania colloidal crystals through evaporation induced self-assembly in emulsion as effective and recyclable photocatalysts

2015 ◽  
Vol 17 (41) ◽  
pp. 27653-27657 ◽  
Author(s):  
Jeffrey E. Chen ◽  
Hong-Yuan Lian ◽  
Saikat Dutta ◽  
Saad M. Alshehri ◽  
Yusuke Yamauchi ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Magnetic Properties ◽  
Self Assembly ◽  
Colloidal Crystal ◽  
Colloidal Crystals ◽  
Mesoporous Titania ◽  
Evaporation Induced Self Assembly

This study illustrates the directed self-assembly of mesoporous TiO2 with magnetic properties due to its colloidal crystal structure with Fe3O4.

scholarly journals Bioinspired hydrogel microfibres colour-encoded with colloidal crystals

2019 ◽  
Vol 6 (9) ◽  
pp. 1938-1943 ◽  
Author(s):  
Zhi-Jun Meng ◽  
Jing Zhang ◽  
Xu Deng ◽  
Ji Liu ◽  
Ziyi Yu ◽  
...  
Keyword(s):  
Colloidal Crystal ◽  
Colloidal Crystals

The integration of microfluidic spinning and colloidal crystal microdots enable the colour-encoded hydrogel microfibres with facile and flexible manipulation of the encoding.

scholarly journals Convenient and Efficient Fabrication of Colloidal Crystals Based on Solidification-Induced Colloidal Assembly

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 575 ◽  
Author(s):  
Ting Shao ◽  
Laixi Sun ◽  
Chun Yang ◽  
Xin Ye ◽  
Shufan Chen ◽  
...  
Keyword(s):  
Driving Force ◽  
Crystallization Process ◽  
Colloidal Crystal ◽  
Colloidal Crystals ◽  
Magnetic Interaction ◽  
Colloidal Assembly ◽  
Water Suspension ◽  
Colloidal Crystallization ◽  
New Strategy ◽  
Crystal Grains

The simple yet efficient and versatile fabrication of colloidal crystals was investigated based on the solidification-induced colloidal crystallization process with particle/water suspension as precursor. The resulting colloidal crystals were constituted by crystal grains with sizes ranging from several tens of micrometers to a few millimeters. Each of the grains had a close-hexagonal array of colloids, which endowed the bulk colloidal crystal powders with some specific optical properties. The freezing of water was shown as the major driving force to form colloidal crystal grains, which supersaturated the solution with nanoparticles and thus induced the formation and growth of colloidal crystal seeds. This process is intrinsically different from those conventional methods based on shearing force, surface tension, columbic interaction or magnetic interaction, revealing a new strategy to fabricate colloidal crystals in a convenient and efficient way.

scholarly journals TGF-β triggers rapid fibrillogenesis via a novel TβRII-dependent fibronectin-trafficking mechanism

2017 ◽  
Vol 28 (9) ◽  
pp. 1195-1207 ◽  
Author(s):  
Archana Varadaraj ◽  
Laura M. Jenkins ◽  
Priyanka Singh ◽  
Anindya Chanda ◽  
John Snider ◽  
...  
Keyword(s):  
Cell Migration ◽  
Transcriptional Control ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Independent Manner ◽  
Ecm Remodeling ◽  
Ecm Proteins ◽  
Β Receptor ◽  
Direct Use ◽  
Α5β1 Integrin

Fibronectin (FN) is a critical regulator of extracellular matrix (ECM) remodeling through its availability and stepwise polymerization for fibrillogenesis. Availability of FN is regulated by its synthesis and turnover, and fibrillogenesis is a multistep, integrin-dependent process essential for cell migration, proliferation, and tissue function. Transforming growth factor β (TGF-β) is an established regulator of ECM remodeling via transcriptional control of ECM proteins. Here we show that TGF-β, through increased FN trafficking in a transcription- and SMAD-independent manner, is a direct and rapid inducer of the fibrillogenesis required for TGF-β–induced cell migration. Whereas TGF-β signaling is dispensable for rapid fibrillogenesis, stable interactions between the cytoplasmic domain of the type II TGF-β receptor (TβRII) and the FN receptor (α5β1 integrin) are required. We find that, in response to TGF-β, cell surface–internalized FN is not degraded by the lysosome but instead undergoes recycling and incorporation into fibrils, a process dependent on TβRII. These findings are the first to show direct use of trafficked and recycled FN for fibrillogenesis, with a striking role for TGF-β in this process. Given the significant physiological consequences associated with FN availability and polymerization, our findings provide new insights into the regulation of fibrillogenesis for cellular homeostasis.

scholarly journals Cell shape, and not 2D migration, predicts ECM-driven 3D cell invasion in breast cancer

2020 ◽  
Author(s):  
Janani P. Baskaran ◽  
Anna Weldy ◽  
Justinne Guarin ◽  
Gabrielle Munoz ◽  
Michael Kotlik ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Tumor Cells ◽  
Cell Invasion ◽  
Cell Shape ◽  
Partial Least Squares Regression ◽  
Tumor Tissue ◽  
Least Squares Regression ◽  
Invasion And Metastasis ◽  
Ecm Proteins

AbstractMetastasis, the leading cause of death in cancer patients, requires the invasion of tumor cells through the stroma in response to migratory cues, such as those provided by the extracellular matrix (ECM). Recent advances in proteomics have led to the identification of hundreds of ECM proteins which are more abundant in tumors relative to healthy tissue. Our goal was to develop a pipeline to easily predict which of these ECM proteins is more likely to have an effect on cancer invasion and metastasis. We evaluated the effect of 4 ECM proteins upregulated in breast tumor tissue in multiple human breast cancer cell lines in 3 assays. We found there was no linear relationship between the 11 cell shape parameters we quantified when cells adhere to ECM proteins and 2D cell migration speed, persistence or 3D invasion. We then used classifiers and partial-least squares regression analysis to identify which metrics best predicted ECM-driven 2D migration and 3D invasion responses. ECM-driven 2D cell migration speed or persistence did not correlate with or predict 3D invasion in response to that same cue. However, cell adhesion, and in particular cell elongation and irregularity accurately predicted the magnitude of ECM-driven 2D migration and 3D invasion in all cell lines. Testing predictions revealed that our models are good at predicting the effect of novel ECM proteins within a given cell line, but that ECM responses are cell-line specific. Overall, our studies identify the cell morphological features that determine 3D invasion responses to individual ECM proteins. This platform will help provide insight into the functional role of ECM proteins abundant tumor tissue and help prioritize strategies for targeting tumor-ECM interactions to treat metastasis.FundingThis work was supported by the National Institutes of Health [R00-CA207866-04 to M.J.O.]; Tufts University [Start-up funds from the School of Engineering to M.J.O.] and funds from NSF REU to A.W.Conflict-of-interest: None.Insight BoxMetastasis, the dissemination of tumor cells, is driven by the interaction of invading tumor cells with their local environment, in particular with the ECM, which provides structure and support to our tissues. This study presents an integrated approach to predict the effect of individual ECM proteins on 3D invasion and metastasis based on simple adhesion assays which quantify cell shape. Machine learning classification and partial-least squares regression models reveal that ECM-driven 2D cell migration metrics are not predictive of 3D invasion, and that cell shape of cells adhered to ECM can predict that protein’s effect on 3D invasion. These data provide a pipeline for predicting the effect of ECM proteins on breast cancer cell invasion and metastasis.

scholarly journals Ordering of binary colloidal crystals by random potentials

Soft Matter ◽  
2020 ◽  
Vol 16 (17) ◽  
pp. 4267-4273 ◽  
Author(s):  
André S. Nunes ◽  
Sabareesh K. P. Velu ◽  
Iryna Kasianiuk ◽  
Denis Kasyanyuk ◽  
Agnese Callegari ◽  
...  
Keyword(s):  
Random Potential ◽  
Colloidal Crystal ◽  
Colloidal Crystals ◽  
Random Potentials

A random potential can control the number of defects in a binary colloidal crystal.

scholarly journals Particle analogs of electrons in colloidal crystals

Science ◽  
2019 ◽  
Vol 364 (6446) ◽  
pp. 1174-1178 ◽  
Author(s):  
Martin Girard ◽  
Shunzhi Wang ◽  
Jingshan S. Du ◽  
Anindita Das ◽  
Ziyin Huang ◽  
...  
Keyword(s):  
Colloidal Crystal ◽  
Colloidal Crystals ◽  
Insulator Transition ◽  
Metal Insulator Transition ◽  
Design Rules ◽  
Metal Insulator ◽  
Grafting Density ◽  
And Diffusion ◽  
Classical Picture

A versatile method for the design of colloidal crystals involves the use of DNA as a particle-directing ligand. With such systems, DNA-nanoparticle conjugates are considered programmable atom equivalents (PAEs), and design rules have been devised to engineer crystallization outcomes. This work shows that when reduced in size and DNA grafting density, PAEs behave as electron equivalents (EEs), roaming through and stabilizing the lattices defined by larger PAEs, as electrons do in metals in the classical picture. This discovery defines a new property of colloidal crystals—metallicity—that is characterized by the extent of EE delocalization and diffusion. As the number of strands increases or the temperature decreases, the EEs localize, which is structurally reminiscent of a metal-insulator transition. Colloidal crystal metallicity, therefore, provides new routes to metallic, intermetallic, and compound phases.

scholarly journals Gravitational Annealing of Colloidal Crystals

2012 ◽  
Vol 323-325 ◽  
pp. 555-558 ◽  
Author(s):  
Yoshihisa Suzuki ◽  
Jin Endoh ◽  
Atsushi Mori ◽  
Tomoki Yabutani ◽  
Katsuhiro Tamura
Keyword(s):  
Colloidal Crystal ◽  
Colloidal Crystals ◽  
Polarized Light ◽  
The Other ◽  
High Gravity ◽  
Silica Colloidal Crystal

A silica colloidal crystal obtained by centrifugation at 9 G for 2 days in water was annealed by additional stronger centrifugation at 50 G for 5 days. The number of the striations observed in the colloidal crystal under crossed polarized light decreased at some parts in a growth container after the additional centrifugation, while the number also increased at the other parts. The decrease probably shows the shrinkage of the stacking disorders under high gravity, while the increase probably shows the production of new stacking disorders.

A New Approach for Colloidal Crystal Fabrication with the Template of Reversible Stress Wrinkle

2011 ◽  
Vol 418-420 ◽  
pp. 651-655
Author(s):  
Qing Xu ◽  
Zhao Ting Yang ◽  
Chao Rong Li ◽  
Ben Yong Chen ◽  
Wen Jun Dong
Keyword(s):  
Large Scale ◽  
Colloidal Crystal ◽  
Colloidal Crystals ◽  
Optical Microscope ◽  
Pdms Substrate ◽  
Mass Ratio ◽  
New Approach ◽  
Defect Rate ◽  
Regular Spacing ◽  
Scanning Electron

Wrinkle is a favorable template for patterning cells or particles. A new approach is developed to control and fabricate PS colloidal crystals with the reversible wrinkle on PDMS substrate/Pt film stressed system. The obtained structure is characterized by optical microscope and scanning electron microscope. It demonstrates that the settlement of colloids can be regulated by the buckling wavelength which can be tuned by varying the film thickness and substrate mixture mass ratio. Compared with monolayer PS film patterned on herringbones pattern wrinkle, the film on stripes pattern wrinkle always has low defect rate. It is meaningful for fabricating large-scale regular spacing and high-ordered PS colloid films.

Photosensitive polystyrene/silver bromide hybrid colloidal crystals as recoverable colorimetric naked eye probes for bromine gas sensing

2016 ◽  
Vol 4 (7) ◽  
pp. 1386-1391 ◽  
Author(s):  
Bing Yu ◽  
Feng Zhai ◽  
Hailin Cong ◽  
Di Yang
Keyword(s):  
Linear Relationship ◽  
Gas Sensing ◽  
Colloidal Crystal ◽  
Colloidal Crystals ◽  
Silver Bromide ◽  
Gas Concentration ◽  
Naked Eye ◽  
Light Yellow ◽  
Reliable Performance ◽  
Deep Green

The color of a hybrid colloidal crystal sensor is changed from deep green to light yellow with increased bromine gas concentrations with a linear relationship between the band gap shift and bromine gas concentration. The sensor has a very reliable performance after repeated sensing and regeneration.

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