Quantitative investigation of negative membrane curvature sensing and generation by I-BARs in filopodia of living cells

Soft Matter ◽  
2019 ◽  
Vol 15 (48) ◽  
pp. 9829-9839 ◽  
Author(s):  
Artù Breuer ◽  
Line Lauritsen ◽  
Elena Bertseva ◽  
Ivana Vonkova ◽  
Dimitrios Stamou
Keyword(s):  
Negative Curvature ◽  
Living Cells ◽  
Membrane Curvature ◽  
Quantitative Investigation ◽  
Curvature Sensing ◽  
Bar Domains

We analyze diffraction-limited filopodia of living cells to quantify negative curvature sensing and generation for two prototypic I-BAR domains.

scholarly journals FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria

PLoS Biology ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. e3001314
Author(s):  
Ane Landajuela ◽  
Martha Braun ◽  
Christopher D. A. Rodrigues ◽  
Alejandro Martínez-Calvo ◽  
Thierry Doan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Mother Cell ◽  
Negative Curvature ◽  
Lipid Binding ◽  
Membrane Curvature ◽  
Membrane Topology ◽  
Cell Cytoplasm ◽  
Curvature Sensing ◽  
Membrane Fission ◽  
Late Stages

Little is known about mechanisms of membrane fission in bacteria despite their requirement for cytokinesis. The only known dedicated membrane fission machinery in bacteria, fission protein B (FisB), is expressed during sporulation in Bacillus subtilis and is required to release the developing spore into the mother cell cytoplasm. Here, we characterized the requirements for FisB-mediated membrane fission. FisB forms mobile clusters of approximately 12 molecules that give way to an immobile cluster at the engulfment pole containing approximately 40 proteins at the time of membrane fission. Analysis of FisB mutants revealed that binding to acidic lipids and homo-oligomerization are both critical for targeting FisB to the engulfment pole and membrane fission. Experiments using artificial membranes and filamentous cells suggest that FisB does not have an intrinsic ability to sense or induce membrane curvature but can bridge membranes. Finally, modeling suggests that homo-oligomerization and trans-interactions with membranes are sufficient to explain FisB accumulation at the membrane neck that connects the engulfment membrane to the rest of the mother cell membrane during late stages of engulfment. Together, our results show that FisB is a robust and unusual membrane fission protein that relies on homo-oligomerization, lipid binding, and the unique membrane topology generated during engulfment for localization and membrane scission, but surprisingly, not on lipid microdomains, negative-curvature lipids, or curvature sensing.

scholarly journals Amphipathic motifs in BAR domains are essential for membrane curvature sensing

2009 ◽  
Vol 28 (21) ◽  
pp. 3303-3314 ◽  
Author(s):  
Vikram K Bhatia ◽  
Kenneth L Madsen ◽  
Pierre-Yves Bolinger ◽  
Andreas Kunding ◽  
Per Hedegård ◽  
...  
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing ◽  
Bar Domains

scholarly journals Membrane curvature sensing by the C-terminal domain of complexin

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
David Snead ◽  
Rachel T. Wragg ◽  
Jeremy S. Dittman ◽  
David Eliezer
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing ◽  
Terminal Domain

Osmotically-induced tension and the binding of N-BAR protein to lipid vesicles

Soft Matter ◽  
2016 ◽  
Vol 12 (8) ◽  
pp. 2465-2472 ◽  
Author(s):  
Jaime B. Hutchison ◽  
Aruni P. K. K. Karunanayake Mudiyanselage ◽  
Robert M. Weis ◽  
Anthony D. Dinsmore
Keyword(s):  
Osmotic Stress ◽  
Binding Affinity ◽  
Lipid Vesicles ◽  
Membrane Curvature ◽  
Protein Domain ◽  
Curvature Sensing

The binding affinity of a curvature-sensing protein domain (N-BAR) is measured as a function of applied osmotic stress while the membrane curvature is nearly constant.

scholarly journals PIP2 Lipids as Regulators of Membrane Curvature Sensing by Enth Domains

2019 ◽  
Vol 116 (3) ◽  
pp. 92a
Author(s):  
Alexis Belessiotis-Richards ◽  
Molly M. Stevens ◽  
Alfredo Alexander-Katz
Keyword(s):  
Membrane Curvature ◽  
Curvature Sensing

scholarly journals Amphipathic helical peptide-based fluorogenic probes for a marker-free analysis of exosomes based on membrane-curvature sensing

RSC Advances ◽  
2020 ◽  
Vol 10 (63) ◽  
pp. 38323-38327
Author(s):  
Yusuke Sato ◽  
Kazuki Kuwahara ◽  
Kenta Mogami ◽  
Kenta Takahashi ◽  
Seiichi Nishizawa
Keyword(s):  
Membrane Curvature ◽  
Helical Peptides ◽  
Curvature Sensing ◽  
Helical Peptide ◽  
Fluorogenic Probes ◽  
Marker Free

Fluorogenic probes based on membrane curvature sensing-amphipathic helical peptides have been developed for a marker-free exosome analysis.

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