Gradient copolymer micelles: an introduction to structures as well as structural transitions

Soft Matter ◽  
2019 ◽  
Vol 15 (27) ◽  
pp. 5357-5370 ◽  
Author(s):  
Chao Zheng
Keyword(s):  
Chain Structure ◽  
Structural Transitions ◽  
Gradient Copolymer ◽  
Copolymer Micelles

Gradient chain structure induced characteristics in the structure as well as the transitions of gradient copolymer micelles.

Swelling and rheology of thermoresponsive gradient copolymer micelles

Soft Matter ◽  
2010 ◽  
Vol 6 (5) ◽  
pp. 984 ◽  
Author(s):  
Nathalie Merlet-Lacroix ◽  
Emanuela Di Cola ◽  
Michel Cloitre
Keyword(s):  
Gradient Copolymer ◽  
Copolymer Micelles

Rapid Isolation of High Molecular Weight Urokinase from Native Human Urine

1982 ◽  
Vol 47 (03) ◽  
pp. 197-202 ◽  
Author(s):  
Kurt Huber ◽  
Johannes Kirchheimer ◽  
Bernd R Binder
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Urine ◽  
Gel Filtration ◽  
Chain Structure ◽  
The Other ◽  
Single Chain ◽  
Apparent Homogeneity ◽  
Sequential Adsorption

SummaryUrokinase (UK) could be purified to apparent homogeneity starting from crude urine by sequential adsorption and elution of the enzyme to gelatine-Sepharose and agmatine-Sepharose followed by gel filtration on Sephadex G-150. The purified product exhibited characteristics of the high molecular weight urokinase (HMW-UK) but did contain two distinct entities, one of which exhibited a two chain structure as reported for the HMW-UK while the other one exhibited an apparent single chain structure. The purification described is rapid and simple and results in an enzyme with probably no major alterations. Yields are high enough to obtain purified enzymes for characterization of UK from individual donors.

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