scholarly journals Single-step multivalent capture assay for nucleic acid detection with dual-affinity regulation using mutation inhibition and allosteric activation

2019 ◽  
Vol 10 (19) ◽  
pp. 5025-5030 ◽  
Author(s):  
Xing Lu ◽  
Guobao Zhou ◽  
Yanbo Zeng ◽  
Zhengzhi Yin ◽  
Zulei Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dynamic Range ◽  
Single Step ◽  
Nucleic Acid Detection ◽  
Allosteric Activation ◽  
Single Nucleotide ◽  
Capture Assay ◽  
Nucleotide Resolution ◽  
Single Nucleotide Resolution

A single-step electrocatalytic biosensor with dual-affinity regulation enables a tunable dynamic range and tunable single nucleotide resolution for nucleic acid detection.

scholarly journals Genomic DNA preparation enabling multiple replicate reads for accurate nanopore sequencing

2015 ◽  
Author(s):  
Dimitra Tsavachidou
Keyword(s):  
Nucleic Acid ◽  
Error Rates ◽  
Nanopore Sequencing ◽  
Acid Molecule ◽  
Single Nucleotide ◽  
Nucleic Acid Molecule ◽  
Dna Strands ◽  
Nucleotide Resolution ◽  
Single Nucleotide Resolution ◽  
Sequencing Procedure

Sequencing at single-nucleotide resolution using nanopore devices is performed with reported error rates 10.5-20.7% (Ip et al., 2015). Since errors occur randomly during sequencing, repeating the sequencing procedure for the same DNA strands several times can generate sequencing results based on consensus derived from replicate readings, thus reducing overall error rates. The method presented in this manuscript constructs copies of a nucleic acid molecule that are consecutively connected to the nucleic acid molecule. Such copies are useful because they can be sequenced by a nanopore device, enabling replicate reads, thus improving overall sequencing accuracy.

scholarly journals Chemical probing of the homopurine·homopyrimidine tract in supercoiled DNA at single-nucleotide resolution

FEBS Letters ◽  
1988 ◽  
Vol 234 (2) ◽  
pp. 295-299 ◽  
Author(s):  
M. Vojtíšková ◽  
S. Mirkin ◽  
V. Lyamichev ◽  
O. Voloshin ◽  
M. Frank-Kamenetskii ◽  
...  
Keyword(s):  
Single Nucleotide ◽  
Supercoiled Dna ◽  
Chemical Probing ◽  
Nucleotide Resolution ◽  
Single Nucleotide Resolution
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