scholarly journals CpG oligodeoxynucleotides inhibit the proliferation and osteoclastic differentiation of RAW264.7 cells

RSC Advances ◽  
2020 ◽  
Vol 10 (25) ◽  
pp. 14885-14891
Author(s):  
Yi Zheng ◽  
Wenwen Yu ◽  
Hongyan Li ◽  
Hongbing Lin ◽  
Zhen Chen ◽  
...  
Keyword(s):  
Raw264.7 Cells ◽  
Cpg Oligodeoxynucleotides ◽  
Bone Absorption ◽  
Prevention And Treatment ◽  
Clinical Prevention ◽  
Osteoclastic Differentiation

Clinical prevention and treatment of periodontitis-induced bone absorption remains a challenge.

scholarly journals Ferryl Hemoglobin Inhibits Osteoclastic Differentiation of Macrophages in Hemorrhaged Atherosclerotic Plaques

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Erzsébet Zavaczki ◽  
Tamás Gáll ◽  
Abolfazl Zarjou ◽  
Zoltán Hendrik ◽  
László Potor ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Carotid Arteries ◽  
Raw264.7 Cells ◽  
Calcium Deposition ◽  
Atherosclerotic Plaques ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa ◽  
Receptor Activator ◽  
Osteoclastic Differentiation ◽  
Human Carotid

Intraplaque hemorrhage frequently occurs in atherosclerotic plaques resulting in cell-free hemoglobin, which is oxidized to ferryl hemoglobin (FHb) in the highly oxidative environment. Osteoclast-like cells (OLCs) derived from macrophages signify a counterbalance mechanism for calcium deposition in atherosclerosis. Our aim was to investigate whether oxidized hemoglobin alters osteoclast formation, thereby affecting calcium removal from mineralized atherosclerotic lesions. RANKL- (receptor activator of nuclear factor kappa-Β ligand-) induced osteoclastogenic differentiation and osteoclast activity of RAW264.7 cells were studied in response to oxidized hemoglobin via assessing bone resorption activity, expression of osteoclast-specific genes, and the activation of signalization pathways. OLCs in diseased human carotid arteries were assessed by immunohistochemistry. FHb, but not ferrohemoglobin, decreased bone resorption activity and inhibited osteoclast-specific gene expression (tartrate-resistant acid phosphatase, calcitonin receptor, and dendritic cell-specific transmembrane protein) induced by RANKL. In addition, FHb inhibited osteoclastogenic signaling pathways downstream of RANK (receptor activator of nuclear factor kappa-Β). It prevented the induction of TRAF6 (tumor necrosis factor (TNF) receptor-associated factor 6) and c-Fos, phosphorylation of p-38 and JNK (c-Jun N-terminal kinase), and nuclear translocation of NFκB (nuclear factor kappa-Β) and NFATc1 (nuclear factor of activated T-cells, cytoplasmic 1). These effects were independent of heme oxygenase-1 demonstrated by knocking down HO-1 gene in RAW264.7 cells and in mice. Importantly, FHb competed with RANK for RANKL binding suggesting possible mechanisms by which FHb impairs osteoclastic differentiation. In diseased human carotid arteries, OLCs were abundantly present in calcified plaques and colocalized with regions of calcium deposition, while the number of these cells were lower in hemorrhagic lesions exhibiting accumulation of FHb despite calcium deposition. We conclude that FHb inhibits RANKL-induced osteoclastic differentiation of macrophages and suggest that accumulation of FHb in a calcified area of atherosclerotic lesion with hemorrhage retards the formation of OLCs potentially impairing calcium resorption.

scholarly journals Negative Regulation of RANKL-induced Osteoclastic Differentiation in RAW264.7 Cells by Estrogen and Phytoestrogens

2005 ◽  
Vol 280 (14) ◽  
pp. 13720-13727 ◽  
Author(s):  
Verónica García Palacios ◽  
Lisa J. Robinson ◽  
Christopher W. Borysenko ◽  
Thomas Lehmann ◽  
Sara E. Kalla ◽  
...  
Keyword(s):  
Negative Regulation ◽  
Raw264.7 Cells ◽  
Osteoclastic Differentiation

scholarly journals Risk factors of endometrial cancer in patients with endometrial hyperplasia: implication for clinical treatments

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jie Zhao ◽  
Yongting Hu ◽  
Yanan Zhao ◽  
Dongmei Chen ◽  
Tingfeng Fang ◽  
...  
Keyword(s):  
Risk Factors ◽  
Endometrial Cancer ◽  
Clinical Characteristics ◽  
Endometrial Hyperplasia ◽  
Endometrial Thickness ◽  
Regression Analyses ◽  
Logistics Regression ◽  
Prevention And Treatment ◽  
Retrospective Study Design ◽  
Clinical Prevention

Abstract Background Endometrial hyperplasia (EH) is commonly-seen in the patients with endometrial cancer (EC), we aimed to evaluated the risk factors of EC in patients with EH, to provide evidence to the clinical prevention and treatment of EC. Methods This study was a retrospective study design. EH patients confirmed by pathological examinations and treated with hysterectomy in our hospital from June 1, 2018 to February 28, 2021 were included. The clinical characteristics of EC and no-EC patients were compared and analyzed. Logistics regression analyses were conducted to evaluate the risk factors of EC in patients with EH. Results A total of 228 EH patients were included, the incidence of EC in the EH patients was 31.58%. There were significant differences in the age, BMI, diabetes, hypertension and pathology of EH between EC and no EC groups (all P < 0.05), no significant differences in the hyperlipidemia, preoperative CA125, number of deliveries, menopause and endometrial thickness between EC and no EC groups were found (all P > 0.05). Logistic regression analyses indicated that age > 50 y (OR 3.064, 95% CI 1.945–5.931), BMI ≥ 25 kg/m2 (OR 2.705, 95% CI 1.121–3.889), diabetes (OR 3.049, 95% CI 1.781–5.114), hypertension (OR 2.725, 95% CI 1.108–3.431) and severe hyperplasia (OR 3.181, 95% CI 1.496–4.228) were the risk factors of EC in patients with EH (all P < 0.05). Conclusions The risk of EC in EH patients is high, especially for those patients with age > 50 y, BMI ≥ 25 kg/m2, diabetes, hypertension and severe hyperplasia, special attentions should be paid for occurrence of EC and early diagnosis and early treatment are needed for those patients.

scholarly journals Chinese expert consensus on clinical prevention and treatment of scar+

2018 ◽  
Vol 6 ◽  
Author(s):  
Kaiyang Lv ◽  
Zhaofan Xia ◽  
Keyword(s):  
Plastic Surgery ◽  
Chinese Patients ◽  
Consensus Panel ◽  
Extensive Experience ◽  
Great Demand ◽  
Asian Patients ◽  
Prevention And Treatment ◽  
Clinical Prevention ◽  
Military Physicians ◽  
Scar Prevention

Abstract Following injury, Asian skin has a tendency toward hyperpigmentation and scar formation than Caucasians. A standardized algorithm tailored to Asian patients, especially Chinese patients, is in great demand. Twelve independent, self-selected academic and military physicians from the department of burn/trauma, plastic surgery and dermatology with extensive experience in treating scars were assembled on January 17, 2015, establishing the consensus panel. This consensus was then appraised, drafted, reviewed, and finalized during the following 3 years, aiming to standardize and improve scar prevention and treatment in China. Hopefully, it may also provide some advices and references for the management of scarring in Asian patients.

Cadmium Stimulates the Osteoclastic Differentiation of RAW264.7 Cells in Presence of Osteoblasts

2011 ◽  
Vol 146 (3) ◽  
pp. 349-353 ◽  
Author(s):  
Xiao Chen ◽  
Guoying Zhu ◽  
Taiyi Jin ◽  
Zhijun Zhou ◽  
Shuzhu Gu ◽  
...  
Keyword(s):  
Raw264.7 Cells ◽  
Osteoclastic Differentiation

Effect of Arthrigia on Adjuvant Arthritis Rats’ IL-12

2011 ◽  
Vol 140 ◽  
pp. 43-47
Author(s):  
Xi Chen ◽  
Cheng Yi Zhang ◽  
Hao Gang Xue
Keyword(s):  
Rheumatoid Arthritis ◽  
Adjuvant Arthritis ◽  
Protective Effects ◽  
Immunological Mechanism ◽  
Rat Models ◽  
Therapeutic Mechanism ◽  
Prevention And Treatment ◽  
Clinical Prevention ◽  
Chronic Autoimmune Disease ◽  
Further Development

Rheumatoid Arthritis （RA） is a systemic chronic autoimmune disease. To study the therapeutic mechanism of effects of Skullcap Brightviolet （SB），glucosamine/chondroitin （GC），and the combination of them (named Arthrigia) on RA. With the adjuvant arthritis (AA) rat models, we observed the protective effects of oral SB, GC and Arthrigia in different concentrations on AA in rats. We further examined the immunological mechanism of the combination of them by testing the rats’ secretion of IL-12. The results showed that Arthrigia inhibited the production and secretion of IL-12 factors in some extent, and its effects are more potent the single SB or GC. The present study provides the experimental evidences for Arthrigia for its further development, clinical prevention and treatment of RA.

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