scholarly journals Synthesis of controlled-size silver nanoparticles for the administration of methotrexate drug and its activity in colon and lung cancer cells

RSC Advances ◽  
2020 ◽  
Vol 10 (18) ◽  
pp. 10646-10660 ◽  
Author(s):  
M. Rozalen ◽  
M. Sánchez-Polo ◽  
M. Fernández-Perales ◽  
T. J. Widmann ◽  
J. Rivera-Utrilla
Keyword(s):  
Lung Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Size Distribution ◽  
Lung Cancer Cells ◽  
Controlled Synthesis ◽  
Narrow Size Distribution ◽  
Capping Agents

A controlled synthesis of methotrexate (MTX) silver nanoparticles (AgNPs-MTX) using borohydride and citrate as reduction and reduction/capping agents, respectively, was performed in order to obtain AgNPs-MTX conjugates with a narrow size distribution.

scholarly journals Anti-cancer activity of biosynthesized silver nanoparticles using Avicennia marina against A549 lung cancer cells through ROS/mitochondrial damages

2020 ◽  
Vol 27 (11) ◽  
pp. 3018-3024 ◽  
Author(s):  
Shan Tian ◽  
Kandasamy Saravanan ◽  
Ramzi A. Mothana ◽  
Govindan Ramachandran ◽  
Govindan Rajivgandhi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Avicennia Marina ◽  
Lung Cancer Cells ◽  
Anti Cancer ◽  
A549 Lung ◽  
Cancer Activity

scholarly journals Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hassan Noorbazargan ◽  
Sobhan Amintehrani ◽  
Aghigh Dolatabadi ◽  
Ainaz Mashayekhi ◽  
Nazanin Khayam ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Lung Cancer ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
X Ray ◽  
Juniperus Chinensis ◽  
Anti Cancer

AbstractThe current study evaluated the anti-cancer properties of bio-functionalized silver nanoparticles fabricated by Juniperus chinensis leaf extracts. The nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, dynamic light scattering, Zeta potential and X-ray spectroscopy. Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. The size of the spherical nanoparticle was 12.96 nm with negative zeta potential. Up-regulation of caspase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, and ROS production indicated the remarkable apoptotic effect of AgNPs compared to cisplatin. Moreover, down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs. Cell cycle analysis and downregulation of cyclin D1 indicated cancer cell cessation in the G0/G1 phase. Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line.

scholarly journals Anticancer Potential of Synthesized Silver Nanoparticles Using Bioactive Metabolites of Actinobacteria Against A549 Lung Cancer Cells

2021 ◽  
Author(s):  
Naushin Bano ◽  
Saba Siddiqui ◽  
Adria Hasan ◽  
Mohammad Amir ◽  
Snober S Mir ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Silver Nanoparticles ◽  
Secondary Metabolites ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Vis Spectroscopy ◽  
A549 Lung

Abstract Multi-drug resistance microorganisms and the rising numbers of cancer cases possess a critical threat to humankind, thereby motivating research on new weapons to combat the problem. To address these issues, researchers are now focusing on secondary metabolites produced by bacteria. Because of having outstanding antibiotic capabilities, actinobacteria are being explored as a potential solution to this problem. Silver nanoparticles of actinobacteria are green, eco-friendly, and cost-effective, as well as having antibacterial and anti-cancer properties and potential use in pharmaceuticals. Antibacterial potential of secondary metabolites produced by actinobacteria namely Microbacterium proteolyticum LA2(R) and Streptomyces rochei LA2(O) has been demonstrated by the well diffusion method. GC-MS was used to detect compounds in bioactive metabolites. The most abundant compound found in metabolites was n-hexadecanoic acid. UV-Vis spectroscopy was used to determine the extracellular development of silver nanoparticles of actinobacteria secondary metabolites, while transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) were used to inspect their morphological appearances, stability, crystalline structure, and coating. The activity of anticancer was assessed using the MTT test to evaluate the cytotoxicity of the secondar metabolites (pure and nanoparticles) against A549 lung cancer cells. We also evaluated the effect of secondar metabolites (pure and nanoparticles) on Reactive oxygen species (ROS) levels, Mitochondrial membrane potential (MMP) and Chromatin condensation through DCFDA, Mito Tracker and DAPI staining. Our result suggested that their secondary metabolites can be used a potential lead compound against cancer. However, more investigation is needed to totally grasp their mechanism of action.

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