scholarly journals Formation of G-quadruplex structure in supercoiled DNA under molecularly crowded conditions

RSC Advances ◽  
2019 ◽  
Vol 9 (45) ◽  
pp. 26248-26251 ◽  
Author(s):  
Dawei Li ◽  
Peiwen Peng ◽  
Zhaoqi Yang ◽  
Bei Lv
Keyword(s):  
Dna Supercoiling ◽  
Molecular Crowding ◽  
Supercoiled Dna ◽  
Circular Dna ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Combined Actions ◽  
Duplex Destabilization

It is demonstrated that G-quadruplex generated from G-rich duplex in a circular DNA as a result of quadruplex stabilization and duplex destabilization created by the combined actions of negative DNA supercoiling and molecular crowding condition.

scholarly journals The Effects of Molecular Crowding on the Structure and Stability of G-Quadruplexes with an Abasic Site

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Takeshi Fujimoto ◽  
Shu-ichi Nakano ◽  
Daisuke Miyoshi ◽  
Naoki Sugimoto
Keyword(s):  
Site Effects ◽  
Enthalpy Change ◽  
Molecular Crowding ◽  
Abasic Site ◽  
Structure And Stability ◽  
Quadruplex Structure ◽  
Abasic Sites ◽  
Crowding Effects ◽  
G Quadruplex ◽  
Quadruplex Formation

Both cellular environmental factors and chemical modifications critically affect the properties of nucleic acids. However, the structure and stability of DNA containing abasic sites under cell-mimicking molecular crowding conditions remain unclear. Here, we investigated the molecular crowding effects on the structure and stability of the G-quadruplexes including a single abasic site. Structural analysis by circular dichroism showed that molecular crowding by PEG200 did not affect the topology of the G-quadruplex structure with or without an abasic site. Thermodynamic analysis further demonstrated that the degree of stabilization of the G-quadruplex by molecular crowding decreased with substitution of an abasic site for a single guanine. Notably, we found that the molecular crowding effects on the enthalpy change for G-quadruplex formation had a linear relationship with the abasic site effects depending on its position. These results are useful for predicting the structure and stability of G-quadruplexes with abasic sites in the cell-mimicking conditions.

Cationic and anionic reverse micelles as the molecular crowding container for G-quadruplex structure

RSC Advances ◽  
2014 ◽  
Vol 4 (39) ◽  
pp. 20531-20534 ◽  
Author(s):  
Meng-Chieh Ho ◽  
Chih-Wei Chang
Keyword(s):  
Reverse Micelles ◽  
Molecular Crowding ◽  
Quadruplex Structure ◽  
Crowding Effects ◽  
G Quadruplex

The d[AG3(T2AG3)3] sequence shows different molecular crowding effects in AOT and CTAB reverse micelles (RMs). Our result indicates that the CTAB RMs are ideal molecular crowding containers for G-quadruplex structure.

Design and Synthesis of an Expanded Porphyrin That Has Selectivity for the c-MYC G-Quadruplex Structure

2005 ◽  
Vol 127 (9) ◽  
pp. 2944-2959 ◽  
Author(s):  
Jeyaprakashnarayanan Seenisamy ◽  
Sridevi Bashyam ◽  
Vijay Gokhale ◽  
Hariprasad Vankayalapati ◽  
Daekyu Sun ◽  
...  
Keyword(s):  
Design And Synthesis ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Expanded Porphyrin

scholarly journals The effects of DNA supercoiling on G-quadruplex formation

2017 ◽  
Vol 45 (21) ◽  
pp. 12069-12079 ◽  
Author(s):  
Doreen A.T. Sekibo ◽  
Keith R. Fox
Keyword(s):  
Dna Supercoiling ◽  
G Quadruplex ◽  
Quadruplex Formation
Sign in / Sign up

Export Citation Format

Share Document