scholarly journals Qualitative and quantitative assessment of genotoxins using SRRz lysis reporter under the control of a newly designed SOS responsive promoter in Escherichia coli

RSC Advances ◽  
2019 ◽  
Vol 9 (61) ◽  
pp. 35662-35670
Author(s):  
Pengfei Yuan ◽  
Junqing Dong ◽  
Weibin Zhao ◽  
Min Zhuo ◽  
Shuang Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quantitative Assessment ◽  
Cell Lysis ◽  
Lambda Phage ◽  
Qualitative And Quantitative ◽  
Lysis Gene ◽  
Responsive Element

A new bacterial genotoxicity detection strain was constructed, in which the cell lysis gene of SRRz from a lambda phage was controlled by a new designed SOS responsive element, designated as Escherichia coli BL21/pUC-PST.

scholarly journals Further tests of a recombination model in which chi removes the RecD subunit from the RecBCD enzyme of Escherichia coli.

Genetics ◽  
1990 ◽  
Vol 126 (3) ◽  
pp. 519-533 ◽  
Author(s):  
F W Stahl ◽  
L C Thomason ◽  
I Siddiqi ◽  
M M Stahl
Keyword(s):  
Escherichia Coli ◽  
Dna Sequence ◽  
Dna Packaging ◽  
Lambda Phage ◽  
Recombination Model ◽  
Phage Types ◽  
Point Of Entry ◽  
Reciprocal Recombination ◽  
Recbcd Enzyme ◽  
Lambda Dna

Abstract When one of two infecting lambda phage types in a replication-blocked cross is chi + and DNA packaging is divorced from the RecBCD-chi interaction, complementary chi-stimulated recombinants are recovered equally in mass lysates only if the chi + parent is in excess in the infecting parental mixture. Otherwise, the chi 0 recombinant is recovered in excess. This observation implies that, along with the chi 0 chromosome, two chi + parent chromosomes are involved in the formation of each chi + recombinant. The trimolecular nature of chi +-stimulated recombination is manifest in recombination between lambda and a plasmid. When lambda recombines with a plasmid via the RecBCD pathway, the resulting chromosome has an enhanced probability of undergoing lambda x lambda recombination in the interval into which the plasmid was incorporated. These two observations support a model in which DNA is degraded by Exo V from cos, the sequence that determines the end of packaged lambda DNA and acts as point of entry for RecBCD enzyme, to chi, the DNA sequence that stimulates the RecBCD enzyme to effect recombination. The model supposes that chi acts by ejecting the RecD subunit from the RecBCD enzyme with two consequences. (1) ExoV activity is blocked leaving a highly recombinagenic, frayed duplex end near chi, and (2) as the enzyme stripped of the RecD subunit travels beyond chi it is competent to catalyze reciprocal recombination.

scholarly journals Qualitative and Quantitative Assessment of the SET HASDM Database

2021 ◽  
Author(s):  
Richard Joseph Licata ◽  
Piyush M Mehta ◽  
W. Kent Tobiska ◽  
Bruce R Bowman ◽  
Marcin Dominik Pilinski
Keyword(s):  
Quantitative Assessment ◽  
Qualitative And Quantitative

scholarly journals The lytB Gene of Escherichia coli Is Essential and Specifies a Product Needed for Isoprenoid Biosynthesis

2001 ◽  
Vol 183 (24) ◽  
pp. 7403-7407 ◽  
Author(s):  
Sean McAteer ◽  
Andrew Coulson ◽  
Neil McLennan ◽  
Millicent Masters
Keyword(s):  
Escherichia Coli ◽  
Protein Structure ◽  
Cell Lysis ◽  
Isoprenoid Biosynthesis ◽  
Globular Protein ◽  
Limited Growth ◽  
E Coli ◽  
Nonmevalonate Pathway ◽  
Tim Barrel

ABSTRACT LytB and GcpE, because they are codistributed with other pathway enzymes, have been predicted to catalyze unknown steps in the nonmevalonate pathway for isoprenoid biosynthesis. We constructed a conditional Escherichia coli lytB mutant and found that LytB is essential for survival and that depletion of LytB results in cell lysis, which is consistent with a role for this protein in isoprenoid biosynthesis. Alcohols which can be converted to pathway intermediates beyond the hypothesized LytB step(s) support limited growth of E. coli lytB mutants. An informatic analysis of protein structure suggested that GcpE is a globular protein of the TIM barrel class and that LytB is also a globular protein. Possible biochemical roles for LytB and GcpE are suggested.

Qualitative and quantitative assessment of urinary cytokeratin 8 and 18 fragments compared with voided urine cytology in diagnosis of bladder carcinoma

Urology ◽  
2004 ◽  
Vol 64 (6) ◽  
pp. 1121-1126 ◽  
Author(s):  
Oliver W. Hakenberg ◽  
Susanne Fuessel ◽  
Katja Richter ◽  
Michael Froehner ◽  
Sven Oehlschlaeger ◽  
...  
Keyword(s):  
Bladder Carcinoma ◽  
Quantitative Assessment ◽  
Urine Cytology ◽  
Qualitative And Quantitative

An Application of Fuzzy Clustering to Customer Portfolio Analysis in Automotive Industry

2016 ◽  
Vol 5 (2) ◽  
pp. 13-25
Author(s):  
Abdulkadir Hiziroglu ◽  
Umit Dursun Senbas
Keyword(s):  
Fuzzy Clustering ◽  
Automotive Industry ◽  
Quantitative Assessment ◽  
Portfolio Analysis ◽  
Matrix Form ◽  
Automotive Supplier ◽  
Qualitative And Quantitative ◽  
Marketing Managers ◽  
The Matrix

Having achieved an optimized customer portfolio has been of significant importance for companies. The literature provides several portfolio models and vast majority of them are in matrix form where several descriptors are used as dimensions of the matrix. These dimensions are characterized in ambiguity and require specific methods to tackle with it. The aim of this paper is to utilize fuzzy clustering in customer portfolio analysis to reduce this uncertainty and to make a comparison with a traditional customer portfolio model. A dataset of 130 customers of an automotive supplier in Turkey is used to perform the analyses and the results are compared with a conventional customer portfolio matrix. By making use of substantiality and balance of portfolio parameters, a qualitative and quantitative assessment of categorization generated by both approaches are evaluated. The use of fuzzy clustering gives more substantial clusters and a more balanced customer portfolio compared to the traditional matrix form of portfolio. Marketing managers can understand their overall customer portfolio better and reduce the effect of descriptive indicators via benefiting the fuzzy clustering results.

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